LOCUS       Exported                9151 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  dCas9 expressed from aTc-inducible promoter in acsA, C RBS: TGGACA.
ACCESSION   .
VERSION     .
KEYWORDS    pCas2C
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9151)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 9151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9151
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      8..55
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     51..69
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(105..4211)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     protein_bind    4279..4296
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             4384..4395
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             4402..5019
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESG"
     primer_bind     complement(4475..4494)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             5023..5055
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     terminator      5103..5174
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5190..5217
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        complement(5777..5795)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5778..5795)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5813..5829)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5813..5829)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5826..5848)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5837..5853
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5861..5891)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5906..5927
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6044..6061)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6215..6803)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6295..6314)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6974..7834)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPEMNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7597..7616
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7835..7939)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8017..8472)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8154..8175)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8366..8385
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8598..8620
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8612..8629
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8613..8629
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8636..8655
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8636..8654
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctctagacaa aaaacccgtc aagacccgtt tagaggcccc aaggggttat gctagttatt
       61 gctcagcggt ggcagcagcc aactcagctt cctttcgggc tttgttagtc acctcctagc
      121 tgactcaaat caatgcgtgt ttcataaaga ccagtgatgg attgatggat aagagtggca
      181 tctaaaactt cttttgtaga cgtatatcgt ttacgatcaa ttgttgtatc aaaatattta
      241 aaagcagcgg gagctccaag attcgtcaac gtaaataaat gaataatatt ttctgcttgt
      301 tcacgtattg gtttgtctct atgtttgtta tatgcactaa gaactttatc taaattggca
      361 tctgctaaaa taacacgctt agaaaattca ctgatttgct caataatctc atctaaataa
      421 tgcttatgct gctccacaaa caattgtttt tgttcgttat cttctggact acccttcaac
      481 ttttcataat gactagctaa atataaaaaa ttcacatatt tgcttggcag agccagctca
      541 tttccttttt gtaattctcc ggcactagcc agcatccgtt tacgaccgtt ttctaactca
      601 aaaagactat atttaggtag tttaatgatt aagtcttttt taacttcctt atatccttta
      661 gcttctaaaa agtcaatcgg atttttttca aaggaacttc tttccataat tgtgatccct
      721 agtaactctt taacggattt taacttcttc gatttccctt tttccacctt agcaaccact
      781 aggactgaat aagctaccgt tggactatca aaaccaccat atttttttgg atcccagtct
      841 tttttacgag caataagctt gtccgaattt ctttttggta aaattgactc cttggagaat
      901 ccgcctgtct gtacttctgt tttcttgaca atattgactt ggggcatgga caatactttg
      961 cgcactgtgg caaaatctcg ccctttatcc cagacaattt ctccagtttc cccattagtt
     1021 tcgattagag ggcgtttgcg aatctctcca tttgcaagtg taatttctgt tttgaagaag
     1081 ttcatgatat tagagtaaaa gaaatatttt gcggttgctt tgcctatttc ttgctcagac
     1141 ttagcaatca ttttacgaac atcataaact ttataatcac catagacaaa ctccgattca
     1201 agttttggat atttcttaat caaagcagtt ccaacgacgg catttagata cgcatcatgg
     1261 gcatgatggt aattgttaat ctcacgtact ttatagaatt ggaaatcttt tcggaagtca
     1321 gaaactaatt tagattttaa ggtaatcact ttaacctctc gaataagttt atcattttca
     1381 tcgtatttag tattcatgcg actatccaaa atttgtgcca catgcttagt gatttggcga
     1441 gtttcaacca attggcgttt gataaaacca gctttatcaa gttcactcaa acctccacgt
     1501 tcagctttcg ttaaattatc aaacttacgt tgagtgatta acttggcgtt tagaagttgt
     1561 ctccaatagt ttttcatctt tttgactact tcttcacttg gaacgttatc cgatttacca
     1621 cgatttttat cagaacgcgt taagacctta ttgtctattg aatcgtcttt aaggaaactt
     1681 tgtggaacaa tggcatcgac atcataatca cttaaacgat taatatctaa ttcttggtcc
     1741 acatacatgt ctcttccatt ttggagataa tagagataga gcttttcatt ttgcaattga
     1801 gtattttcaa caggatgctc tttaagaatc tgacttccta attctttgat accttcttcg
     1861 attcgtttca tacgctctcg cgaatttttc tggccctttt gagttgtctg attttcacgt
     1921 gccatttcaa taacgatatt ttctggctta tgccgcccca ttactttgac caattcatca
     1981 acaactttta cagtctgtaa aatacctttt ttaatagcag ggctaccagc taaatttgca
     2041 atatgttcat gtaaactatc gccttgtcca gacacttgtg ctttttgaat gtcttcttta
     2101 aatgtcaaac tatcatcatg gatcagctgc ataaaattgc gattggcaaa accatctgat
     2161 ttcaaaaaat ctaatattgt tttgccagat tgcttatccc taataccatt aatcaatttt
     2221 cgagacaaac gtccccaacc agtataacgg cgacgtttaa gctgtttcat caccttatca
     2281 tcaaagaggt gagcatatgt tttaagtctt tcctcaatca tctccctatc ttcaaataag
     2341 gtcaatgtta aaacaatatc ctctaagata tcttcatttt cttcattatc caaaaaatct
     2401 ttatctttaa taatttttag caaatcatgg taggtaccta atgaagcatt aaatctatct
     2461 tcaactcctg aaatttcaac actatcaaaa cattctattt ttttgaaata atcttctttt
     2521 aattgcttaa cggttacttt tcgatttgtt ttgaagagta aatcaacaat ggctttcttc
     2581 tgttcacctg aaagaaatgc tggttttcgc attccttcag taacatattt gacctttgtc
     2641 aattcgttat aaaccgtaaa atactcataa agcaaactat gttttggtag tactttttca
     2701 tttggaagat ttttatcaaa gtttgtcatg cgttcaataa atgattgagc tgaagcacct
     2761 ttatcgacaa cttcttcaaa attccatggg gtaattgttt cttcagactt ccgagtcatc
     2821 catgcaaaac gactattgcc acgcgccaat ggaccaacat aataaggaat tcgaaaagtc
     2881 aagatttttt caatcttctc acgattgtct tttaaaaatg gataaaagtc ttcttgtctt
     2941 ctcaaaatag catgcagctc acccaagtga atttgatggg gaatagagcc gttgtcaaag
     3001 gtccgttgct tgcgcagcaa atcttcacga tttagtttca ccaataattc ctcagtacca
     3061 tccatttttt ctaaaattgg tttgataaat ttataaaatt cttcttggct agctccccca
     3121 tcaatataac ctgcatatcc gttttttgat tgatcaaaaa agatttcttt atacttttct
     3181 ggaagttgtt gtcgaactaa agcttttaaa agagtcaagt cttgatgatg ttcatcgtag
     3241 cgtttaatca ttgaagctga taggggagcc ttagttattt cagtatttac tcttaggata
     3301 tctgaaagta aaatagcatc tgataaattc ttagctgcca aaaacaaatc agcatattga
     3361 tctccaattt gcgccaataa attatctaaa tcatcatcgt aagtatcttt tgaaagctgt
     3421 aatttagcat cttctgccaa atcaaaattt gatttaaaat taggggtcaa acccaatgac
     3481 aaagcaatga gattcccaaa taatccattt ttcttctcac cggggagctg agcaatgaga
     3541 ttttctaatc gtcttgattt actcaatcgt gcagaaagaa tcgctttagc atctactcca
     3601 cttgcgttaa tagggttttc ttcaaataat tgattgtagg tttgtaccaa ctggataaat
     3661 agtttgtcca catcactatt atcaggattt aaatctccct caatcaaaaa atgaccacga
     3721 aacttaatca tatgcgctaa ggccaaatag attaagcgca aatccgcttt atcagtagaa
     3781 tctaccaatt tttttcgcag atgatagata gttggatatt tctcatgata agcaacttca
     3841 tctactatat ttccaaaaat aggatgacgt tcatgcttct tgtcttcttc caccaaaaaa
     3901 gactcttcaa gtcgatgaaa gaaactatca tctactttcg ccatctcatt tgaaaaaatc
     3961 tcctgtagat aacaaatacg attcttccga cgtgtatacc ttctacgagc tgtccgtttg
     4021 agacgagtcg cttccgctgt ctctccactg tcaaataaaa gagcccctat aagatttttt
     4081 ttgatactgt ggcggtctgt atttcccaga accttgaact ttttagacgg aaccttatat
     4141 tcatcagtga tcaccgccca tccgacgcta tttgtgccga tagctaagcc tattgagtat
     4201 ttcttatcca tggaattaat gtccaacttg actttatgag ttgggagctc cttttgcaat
     4261 cccacagtta actttatact ctatcactga tagggatgtc tgtctatcac tgatagggat
     4321 attcctgctt ttttgttaac ctgatggcta gctcagtcct agggattatg ctagctacta
     4381 gagaaagagg agaaatacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca
     4441 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag
     4501 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac
     4561 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa
     4621 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat
     4681 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc
     4741 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca
     4801 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc
     4861 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct
     4921 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc
     4981 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac
     5041 tacgctttag tagcttaata acactgatag tgctagtgta gatcactact agagccaggc
     5101 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     5161 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     5221 atagttcgtt tcaatgaagg cgaaacgccc ctgttgaccc gattcatcca tttcaatttg
     5281 ggccacaaaa aattccttct ggacaatttc cccttcctct gtgaaggaaa tttcacccaa
     5341 aggcgtgacg taggtacctg caaaaatttc gtcccgcagt tgtcgtcgca agtccggtag
     5401 agcaagagtt tctaagggcg ttttttcatc gaggctgctg agggcttcaa caaaaacttg
     5461 gatcgccgta aaagcctggg cactaaattg gggcggctct ttttggtttt gttgaaaata
     5521 ggcgtcccga aacgcgcggt taatctcatt atctaactcg gcactgtagg cttgggccac
     5581 caacacccca tcacattttg cttggcagac ggggaaaata ttagaagtat ttaagccgtt
     5641 ccccccaaca ataatgccgt tgtaacctaa ttctcgcagt tgcttcacca ggttgcccgc
     5701 atcggctgct aaccccgaaa taatcactag atctggttga acattgagtg cggcagtcac
     5761 ctcatagctt gagtattcta tagtgtcacc taaatagctt ggcgtaatca tggtcatagc
     5821 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     5881 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     5941 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6001 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6061 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6121 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6181 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6241 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6301 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6361 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6421 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6481 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6541 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6601 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     6661 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6721 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     6781 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     6841 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     6901 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     6961 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7021 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7081 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7141 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7201 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7261 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7321 gtatggcttc attcatctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7381 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7441 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7501 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7561 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7621 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7681 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7741 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     7801 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     7861 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     7921 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgatgcg gtgtgaaata
     7981 ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt
     8041 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     8101 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     8161 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     8221 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     8281 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     8341 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     8401 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     8461 tacagggcgc gtccattcgc cattcaggct gcacaactgt tgggaagggc gatcggtgcg
     8521 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     8581 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata
     8641 cgactcacta tagggcgcat gctgaacaac tttggctgcc gtcccaccat cgccaaccca
     8701 agcaacggcg atcgccacgg tgcaagacaa ttgggacatg gccctagccc aacagcccaa
     8761 cgaccccgaa attttgatgg ccctgggttt tgtccattac ctcaatgcag attacgaagc
     8821 cgccaatgat cgttggtatg aagccttagc ggcggcaacg acccaggatt tccccccagc
     8881 cctccaggcg gagatttatg cgggcctggc gattagtatg tacgatttgg cccaaaggca
     8941 acccaccgac gttgaccagg aaattcttgt agggaaagcc gaaaaactcc gggcgatcgc
     9001 cctagatctc gatcgttccc agctcaaccc ccgccgcctc gaacaaagtt ggttatggtt
     9061 ccctgaggcg atcgccactt ggcaaaccct gctcgcttta gaagaatctc cctaggtcat
     9121 atccggggca tacattcgat caacttgagc g
//
