LOCUS       Exported                4182 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Gateway Donor vector containing RARA, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    pDONR223_RARA_WT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2061..2160)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2177..2196)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2178..2196)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2201..2217)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2201..2217)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2648..3439
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3532..4120
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4021..4040
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggccagca acagcagctc ctgcccgaca cctgggggcg ggcacctcaa
      721 tgggtacccg gtgcctccct acgccttctt cttcccccct atgctgggtg gactctcccc
      781 gccaggcgct ctgaccactc tccagcacca gcttccagtt agtggatata gcacaccatc
      841 cccagccacc attgagaccc agagcagcag ttctgaagag atagtgccca gccctccctc
      901 gccaccccct ctaccccgca tctacaagcc ttgctttgtc tgtcaggaca agtcctcagg
      961 ctaccactat ggggtcagcg cctgtgaggg ctgcaagggc ttcttccgcc gcagcatcca
     1021 gaagaacatg gtgtacacgt gtcaccggga caagaactgc atcatcaaca aggtgacccg
     1081 gaaccgctgc cagtactgcc gactgcagaa gtgctttgaa gtgggcatgt ccaaggagtc
     1141 tgtgagaaac gaccgaaaca agaagaagaa ggaggtgccc aagcccgagt gctctgagag
     1201 ctacacgctg acgccggagg tgggggagct cattgagaag gtgcgcaaag cgcaccagga
     1261 aaccttccct gccctctgcc agctgggcaa atacactacg aacaacagct cagaacaacg
     1321 tgtctctctg gacattgacc tctgggacaa gttcagtgaa ctctccacca agtgcatcat
     1381 taagactgtg gagttcgcca agcagctgcc cggcttcacc accctcacca tcgccgacca
     1441 gatcaccctc ctcaaggctg cctgcctgga catcctgatc ctgcggatct gcacgcggta
     1501 cacgcccgag caggacacca tgaccttctc ggacgggctg accctgaacc ggacccagat
     1561 gcacaacgct ggcttcggcc ccctcaccga cctggtcttt gccttcgcca accagctgct
     1621 gcccctggag atggatgatg cggagacggg gctgctcagc gccatctgcc tcatctgcgg
     1681 agaccgccag gacctggagc agccggaccg ggtggacatg ctgcaggagc cgctgctgga
     1741 ggcgctaaag gtctacgtgc ggaagcggag gcccagccgc ccccacatgt tccccaagat
     1801 gctaatgaag attactgacc tgcgaagcat cagcgccaag ggggctgagc gggtgatcac
     1861 gctgaagatg gagatcccgg gctccatgcc gcctctcatc caggaaatgt tggagaactc
     1921 agagggcctg gacactctga gcggacagcc ggggggtggg gggcgggacg ggggtggcct
     1981 ggcccccccg ccaggcagct gtagccccag cctcagcccc agctccaaca gaagcagccc
     2041 ggccacccac tccccgtgat tgccaacttt cttgtacaaa gttggcatta taagaaagca
     2101 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     2161 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     2221 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     2281 atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc
     2341 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc
     2401 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
     2461 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     2521 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     2581 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     2641 aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     2701 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     2761 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
     2821 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     2881 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     2941 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     3001 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     3061 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     3121 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     3181 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     3241 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     3301 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     3361 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
     3421 caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta
     3481 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3541 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3601 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3661 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3721 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3781 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3841 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3901 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3961 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4021 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4081 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4141 tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
