LOCUS Exported 6000 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.. ACCESSION . VERSION . KEYWORDS pDONR223_PIK3CA_p.E600K SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6000) AUTHORS Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS TITLE High-throughput Phenotyping of Lung Cancer Somatic Mutations. JOURNAL Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28. PUBMED 27478040 REFERENCE 2 (bases 1 to 6000) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6000 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 43..65 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 57..74 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 58..74 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 90..189 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(3400..3499) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(3516..3535) /label=T7 /note="T7 promoter, forward primer" promoter complement(3517..3535) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3540..3556) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3540..3556) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 3987..4778 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 4871..5459 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5360..5379 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 5613..5630 /label=L4440 /note="L4440 vector, forward primer" terminator 5789..5816 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 5908..5994 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta 61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga 121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa 181 aagttggcac catgcctcca cgaccatcat caggtgaact gtggggcatc cacttgatgc 241 ccccaagaat cctagtagaa tgtttactac caaatggaat gatagtgact ttagaatgcc 301 tccgtgaggc tacattaata accataaagc atgaactatt taaagaagca agaaaatacc 361 ccctccatca acttcttcaa gatgaatctt cttacatttt cgtaagtgtt actcaagaag 421 cagaaaggga agaatttttt gatgaaacaa gacgactttg tgaccttcgg ctttttcaac 481 cctttttaaa agtaattgaa ccagtaggca accgtgaaga aaagatcctc aatcgagaaa 541 ttggttttgc tatcggcatg ccagtgtgtg aatttgatat ggttaaagat ccagaagtac 601 aggacttccg aagaaatatt ctgaacgttt gtaaagaagc tgtggatctt agggacctca 661 attcacctca tagtagagca atgtatgtct atcctccaaa tgtagaatct tcaccagaat 721 tgccaaagca catatataat aaattagata aagggcaaat aatagtggtg atctgggtaa 781 tagtttctcc aaataatgac aagcagaagt atactctgaa aatcaaccat gactgtgtac 841 cagaacaagt aattgctgaa gcaatcagga aaaaaactcg aagtatgttg ctatcctctg 901 aacaactaaa actctgtgtt ttagaatatc agggcaagta tattttaaaa gtgtgtggat 961 gtgatgaata cttcctagaa aaatatcctc tgagtcagta taagtatata agaagctgta 1021 taatgcttgg gaggatgccc aatttgatgt tgatggctaa agaaagcctt tattctcaac 1081 tgccaatgga ctgttttaca atgccatctt attccagacg catttccaca gctacaccat 1141 atatgaatgg agaaacatct acaaaatccc tttgggttat aaatagtgca ctcagaataa 1201 aaattctttg tgcaacctac gtgaatgtaa atattcgaga cattgataag atctatgttc 1261 gaacaggtat ctaccatgga ggagaaccct tatgtgacaa tgtgaacact caaagagtac 1321 cttgttccaa tcccaggtgg aatgaatggc tgaattatga tatatacatt cctgatcttc 1381 ctcgtgctgc tcgactttgc ctttccattt gctctgttaa aggccgaaag ggtgctaaag 1441 aggaacactg tccattggca tggggaaata taaacttgtt tgattacaca gacactctag 1501 tatctggaaa aatggctttg aatctttggc cagtacctca tggattagaa gatttgctga 1561 accctattgg tgttactgga tcaaatccaa ataaagaaac tccatgctta gagttggagt 1621 ttgactggtt cagcagtgtg gtaaagttcc cagatatgtc agtgattgaa gagcatgcca 1681 attggtctgt atcccgagaa gcaggattta gctattccca cgcaggactg agtaacagac 1741 tagctagaga caatgaatta agggaaaatg acaaagaaca gctcaaagca atttctacac 1801 gagatcctct ctctgaaatc actgagcagg agaaagattt tctatggagt cacagacact 1861 attgtgtaac tatccccgaa attctaccca aattgcttct gtctgttaaa tggaattcta 1921 gagatgaagt agcccagatg tattgcttgg taaaagattg gcctccaatc aaacctgaac 1981 aggctatgaa acttctggac tgtaattacc cagatcctat ggttcgaggt tttgctgttc 2041 ggtgcttgga aaaatattta acagatgaca aactttctca gtatttaatt cagctagtac 2101 aggtcctaaa atatgaacaa tatttggata acttgcttgt gagattttta ctgaagaaag 2161 cattgactaa tcaaaggatt gggcactttt tcttttggca tttaaaatct gagatgcaca 2221 ataaaacagt tagccagagg tttggcctgc ttttggagtc ctattgtcgt gcatgtggga 2281 tgtatttgaa gcacctgaat aggcaagtcg aggcaatgga aaagctcatt aacttaactg 2341 acattctcaa acaggagaag aaggatgaaa cacaaaaggt acagatgaag tttttagttg 2401 agcaaatgag gcgaccagat ttcatggatg ctctacaggg ctttctgtct cctctaaacc 2461 ctgctcatca actaggaaac ctcaggcttg aagagtgtcg aattatgtcc tctgcaaaaa 2521 ggccactgtg gttgaattgg gagaacccag acatcatgtc agagttactg tttcagaaca 2581 atgagatcat ctttaaaaat ggggatgatt tacggcaaga tatgctaaca cttcaaatta 2641 ttcgtattat ggaaaatatc tggcaaaatc aaggtcttga tcttcgaatg ttaccttatg 2701 gttgtctgtc aatcggtgac tgtgtgggac ttattgaggt ggtgcgaaat tctcacacta 2761 ttatgcaaat tcagtgcaaa ggcggcttga aaggtgcact gcagttcaac agccacacac 2821 tacatcagtg gctcaaagac aagaacaaag gagaaatata tgatgcagcc attgacctgt 2881 ttacacgttc atgtgctgga tactgtgtag ctaccttcat tttgggaatt ggagatcgtc 2941 acaatagtaa catcatggtg aaagacgatg gacaactgtt tcatatagat tttggacact 3001 ttttggatca caagaagaaa aaatttggtt ataaacgaga acgtgtgcca tttgttttga 3061 cacaggattt cttaatagtg attagtaaag gagcccaaga atgcacaaag acaagagaat 3121 ttgagaggtt tcaggagatg tgttacaagg cttatctagc tattcgacag catgccaatc 3181 tcttcataaa tcttttctca atgatgcttg gctctggaat gccagaacta caatcttttg 3241 atgacattgc atacattcga aagaccctag ccttagataa aactgagcaa gaggctttgg 3301 agtatttcat gaaacaaatg aatgatgcac atcatggtgg ctggacaaca aaaatggatt 3361 ggatcttcca cacaattaaa cagcatgcat tgaactgatt gccaactttc ttgtacaaag 3421 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc 3481 aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg 3541 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac 3601 aagataaaaa tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt 3661 cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc 3721 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca 3781 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg 3841 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc 3901 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca 3961 gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc 4021 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac 4081 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg 4141 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg 4201 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg 4261 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat 4321 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg 4381 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg 4441 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc 4501 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc 4561 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg 4621 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc 4681 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact 4741 acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa 4801 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca 4861 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 4921 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 4981 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag 5041 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 5101 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 5161 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 5221 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 5281 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 5341 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 5401 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 5461 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 5521 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg 5581 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag 5641 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 5701 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg 5761 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt 5821 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt 5881 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa 5941 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct //