LOCUS       Exported                4095 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Gateway Donor vector containing STK11, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    pDONR223_STK11_p.D176H
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1495..1594)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1611..1630)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1612..1630)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1635..1651)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1635..1651)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2082..2873
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2966..3554
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3455..3474
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3708..3725
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      3884..3911
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      4003..4089
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggaggtg gtggacccgc agcagctggg catgttcacg gagggcgagc
      241 tgatgtcggt gggtatggac acgttcatcc accgcatcga ctccaccgag gtcatctacc
      301 agccgcgccg caagcgggcc aagctcatcg gcaagtacct gatgggggac ctgctggggg
      361 aaggctctta cggcaaggtg aaggaggtgc tggactcgga gacgctgtgc aggagggccg
      421 tcaagatcct caagaagaag aagttgcgaa ggatccccaa cggggaggcc aacgtgaaga
      481 aggaaattca actactgagg aggttacggc acaaaaatgt catccagctg gtggatgtgt
      541 tatacaacga agagaagcag aaaatgtata tggtgatgga gtactgcgtg tgtggcatgc
      601 aggaaatgct ggacagcgtg ccggagaagc gtttcccagt gtgccaggcc cacgggtact
      661 tctgtcagct gattgacggc ctggagtacc tgcatagcca gggcattgtg cacaagcaca
      721 tcaagccggg gaacctgctg ctcaccaccg gtggcaccct caaaatctcc gacctgggcg
      781 tggccgaggc actgcacccg ttcgcggcgg acgacacctg ccggaccagc cagggctccc
      841 cggctttcca gccgcccgag attgccaacg gcctggacac cttctccggc ttcaaggtgg
      901 acatctggtc ggctggggtc accctctaca acatcaccac gggtctgtac cccttcgaag
      961 gggacaacat ctacaagttg tttgagaaca tcgggaaggg gagctacgcc atcccgggcg
     1021 actgtggccc cccgctctct gacctgctga aagggatgct tgagtacgaa ccggccaaga
     1081 ggttctccat ccggcagatc cggcagcaca gctggttccg gaagaaacat cctccggctg
     1141 aagcaccagt gcccatccca ccgagcccag acaccaagga ccggtggcgc agcatgactg
     1201 tggtgccgta cttggaggac ctgcacggcg cggacgagga cgaggacctc ttcgacatcg
     1261 aggatgacat catctacact caggacttca cggtgcccgg acaggtccca gaagaggagg
     1321 ccagtcacaa tggacagcgc cggggcctcc ccaaggccgt gtgtatgaac ggcacagagg
     1381 cggcgcagct gagcaccaaa tccagggcgg agggccgggc ccccaaccct gcccgcaagg
     1441 cctgctccgc cagcagcaag atccgccggc tgtcggcctg caagcagcag tgattgccaa
     1501 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
     1561 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
     1621 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     1681 gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca
     1741 gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga
     1801 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     1861 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     1921 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     1981 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     2041 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg
     2101 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     2161 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     2221 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     2281 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     2341 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     2401 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     2461 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     2521 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     2581 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     2641 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     2701 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     2761 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     2821 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga
     2881 gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     2941 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3001 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3061 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3121 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3181 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3241 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3301 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3361 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3421 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3481 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3541 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3601 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3661 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3721 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     3781 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     3841 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     3901 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     3961 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     4021 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg
     4081 ttttatttga tgcct
//
