LOCUS       Exported                 987 bp ds-DNA     linear   SYN 26-MAY-2021
DEFINITION  Bioluminescent Reporter of Intracellular Calcium.
ACCESSION   .
VERSION     .
KEYWORDS    pT7-CalfluxVTN
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Yang J, Cumberbatch D, Centanni S, Shi SQ, Winder D, Webb D, Johnson
            CH
  TITLE     Coupling optogenetic stimulation with NanoLuc-based luminescence 
            (BRET) Ca++ sensing.
  JOURNAL   Nat Commun. 2016 Oct 27;7:13268. doi: 10.1038/ncomms13268.
  PUBMED    27786307
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 26, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     216..233
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     318..337
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        318..336
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             368..390
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             410..427
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             446..964
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(491..512)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(752..771)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
       61 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      121 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      181 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      241 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagga
      301 tctcgatccc gcgaaattaa tacgactcac tatagggaga ccacaacggt ttccctctag
      361 aaataatttt gtttaacttt aagaaggaga tatacatatg cggggttctc atcatcatca
      421 tcatcatggt atggctagcg aattcatggt gagcaagggc gaggagctgt tcaccggggt
      481 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      541 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg
      601 caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt
      661 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
      721 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
      781 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
      841 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
      901 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
      961 cgaggacggc ggcgtgcagc tcgccga
//
