LOCUS Exported 1010 bp ds-DNA linear SYN 23-MAY-2021 DEFINITION Bioluminescent Reporter of Intracellular Calcium. ACCESSION . VERSION . KEYWORDS pT7-CalfluxVTN SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1010) AUTHORS Yang J, Cumberbatch D, Centanni S, Shi SQ, Winder D, Webb D, Johnson CH TITLE Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing. JOURNAL Nat Commun. 2016 Oct 27;7:13268. doi: 10.1038/ncomms13268. PUBMED 27786307 REFERENCE 2 (bases 1 to 1010) AUTHORS . TITLE Direct Submission JOURNAL Exported May 23, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1010 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(40..555) /codon_start=1 /product="NanoLuc(R) luciferase" /label=Nluc /note="human codon-optimized" /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW RLCERILA" primer_bind complement(802..823) /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 tcagcttcct ttcgggcttt gttagcagcc ggatcaagct tacgccagaa tgcgttcgca 61 cagccgccag ccggtcactc cgttgatggt tactcggaac agcagggagc cgtcggggtt 121 gatcaggcgc tcgtcgataa ttttgttgcc gttccacagg gtccctgtta cagtgatctt 181 tttgccgtcg aacacggcga tgccttcata cggccgtccg aaatagtcga tcatgttcgg 241 cgtaaccccg tcgattacca gtgtgccata gtgcaggatc accttaaagt gatgatcatc 301 cacagggtac accaccttaa aaattttttc gatctggccc atttggtcgc cgctcagacc 361 ttcatacggg atgatgacat ggatgtcgat cttcagccca ttttcaccgc tcaggacaat 421 cctttggatc ggagttacgg acaccccgag attctgaaac aaactggaca cacctccctg 481 ttcaaggact tggtccaggt tgtagccggc tgtctgtcgc cagtccccaa cgaaatcttc 541 gagtgtgaag accatgagct cggccagagg ctggacattc tccaccatct tcaggaactc 601 atcaaagtca atccttccat ttttgtctgt gtctgaatct ccaaatatct catcgggatc 661 ctcatctgtg agctgttctc cagtaaggcg gatgatgtct ccaaattcct ccctgtcgat 721 gaaaccattt ccatccttgt caaaaatgcg aaagcactct gatagctctt cttcgctagc 781 atcttgcatg cgggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt 841 ggggtctttg ctcagggcgg actggtagct caggtagtgg ttgtcgggca gcagcacggg 901 gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc cgccgtcctc 961 gatgttgtgg cggatcttga agttggcctt gatgccgttc ttctgcttgt //