LOCUS Exported 760 bp ds-DNA linear SYN 25-MAY-2021 DEFINITION expression of SRSF2 in mammalian cells. ACCESSION . VERSION . KEYWORDS pRRL_SRSF2_WT_mCherry SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 760) AUTHORS Kim E, Ilagan JO, Liang Y, Daubner GM, Lee SC, Ramakrishnan A, Li Y, Chung YR, Micol JB, Murphy ME, Cho H, Kim MK, Zebari AS, Aumann S, Park CY, Buonamici S, Smith PG, Deeg HJ, Lobry C, Aifantis I, Modis Y, Allain FH, Halene S, Bradley RK, Abdel-Wahab O TITLE SRSF2 Mutations Contribute to Myelodysplasia by Mutant-Specific Effects on Exon Recognition. JOURNAL Cancer Cell. 2015 May 11;27(5):617-30. doi: 10.1016/j.ccell.2015.04.006. PUBMED 25965569 REFERENCE 2 (bases 1 to 760) AUTHORS . TITLE Direct Submission JOURNAL Exported May 25, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..760 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(102..155) /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS complement(156..179) /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" ORIGIN 1 atctcgaact cgtggccgtt cacggagccc tccatgtgca ccttgaagcg catgaactcc 61 ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca tagggccggg attctcctcc 121 acgtcaccgc atgttagaag acttcctctg ccctccttgt cgtcatcgtc tttgtagtcg 181 ccgccagagg acaccgctcc ttcctcttca ggagacttgg ggggactctt cgatcgcgac 241 ctggatttgg attccctctt ggacactggg ggaggactcc tggaccgaga ccgggacctg 301 gaccgcgaac gagatctgga gaccgacgag gacttggact tggaccttcg tgcggatctg 361 gacttggagg tcgaccgaga tcgagaacga gtgcgggacc gagacttcga gcggctgtag 421 cgagatcggc tgcgagacct ggaacggctc cgactccggg atcggctgcg gcgacgccgc 481 ctagggctgc ggctccggcg tccgtagcca ccgcccccgt acctgcgggg tggcggtccc 541 cggcggctgt ggtgtgagtc cggggggcgg ccgtagcgcg ccatttgcac ccgcagctcg 601 cggccgtcca gcacggcccc gtccatggca tccatagcgt cctcagcgtc gcgcttgtcg 661 tgaaagcgaa cgaaggcgaa gccgcgggac tccttggtgt agcgatcccg cgggatgtac 721 acgtcgccga cgcgcccgta cttctcgaag acgcgcctca //