LOCUS Exported 3696 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway Donor vector containing STC2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.. ACCESSION . VERSION . KEYWORDS pDONR223_STC2_WT_V5 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3696) AUTHORS Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS TITLE High-throughput Phenotyping of Lung Cancer Somatic Mutations. JOURNAL Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28. PUBMED 27478040 REFERENCE 2 (bases 1 to 3696) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3696 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 43..65 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 57..74 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 58..74 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 90..189 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1096..1195) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1212..1231) /label=T7 /note="T7 promoter, forward primer" promoter complement(1213..1231) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1236..1252) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1236..1252) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 1683..2474 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 2567..3155 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3056..3075 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3309..3326 /label=L4440 /note="L4440 vector, forward primer" terminator 3485..3512 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3604..3690 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta 61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga 121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa 181 aagttggcat gtgtgccgag cggctgggcc agttcatgac cctggctttg gtgttggcca 241 cctttgaccc ggcgcggggg accgacgcca ccaacccacc cgagggtccc caagacagga 301 gctcccagca gaaaggccgc ctgtccctgc agaatacagc ggagatccag cactgtttgg 361 tcaacgctgg cgatgtgggg tgtggcgtgt ttgaatgttt cgagaacaac tcttgtgaga 421 ttcggggctt acatgggatt tgcatgactt ttctgcacaa cgctggaaaa tttgatgccc 481 agggcaagtc attcatcaaa gacgccttga aatgtaaggc ccacgctctg cggcacaggt 541 tcggctgcat aagccggaag tgcccggcca tcagggaaat ggtgtcccag ttgcagcggg 601 aatgctacct caagcacgac ctgtgcgcgg ctgcccagga gaacacccgg gtgatagtgg 661 agatgatcca tttcaaggac ttgctgctgc acgaacccta cgtggacctc gtgaacttgc 721 tgctgacctg tggggaggag gtgaaggagg ccatcaccca cagcgtgcag gttcagtgtg 781 agcagaactg gggaagcctg tgctccatct tgagcttctg cacctcggcc atccagaagc 841 ctcccacggc gccccccgag cgccagcccc aggtggacag aaccaagctc tccagggccc 901 accacgggga agcaggacat cacctcccag agcccagcag tagggagact ggccgaggtg 961 ccaagggtga gcgaggtagc aagagccacc caaacgccca tgcccgaggc agagtcgggg 1021 gccttggggc tcagggacct tccggaagca gcgagtggga agacgaacag tctgagtatt 1081 ctgatatccg gaggtgccca actttcttgt acaaagttgg cattataaga aagcattgct 1141 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc 1201 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct 1261 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc 1321 tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt 1381 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc 1441 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac 1501 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg 1561 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga 1621 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca 1681 ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca 1741 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg 1801 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg 1861 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga 1921 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc 1981 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag 2041 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag 2101 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac 2161 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg 2221 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg 2281 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt 2341 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg 2401 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg 2461 tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt 2521 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 2581 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 2641 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 2701 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 2761 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 2821 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 2881 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 2941 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 3001 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 3061 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 3121 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 3181 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 3241 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 3301 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc 3361 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 3421 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa 3481 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg 3541 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg 3601 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca 3661 gtcttccgac tgagcctttc gttttatttg atgcct //