LOCUS Exported 4731 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: C1 Cloning Vector, Excitation: 487, Emission: 509. ACCESSION . VERSION . KEYWORDS mEmerald-C1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4731) TITLE Davidson mEmerald REFERENCE 2 (bases 1 to 4731) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4731 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 16..319 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 320..523 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 474..494 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 562..571 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 568..1284 /codon_start=1 /product="brightly fluorescent monomeric variant of GFP" /label=mEmerald /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(613..634) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(874..893) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1221..1242 /label=EGFP-C /note="EGFP, forward primer" misc_feature 1285..1350 /label=MCS /note="multiple cloning site" polyA_signal 1474..1595 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1511..1530) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1565..1584 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1602..2057) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1739..1760) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1951..1970 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2084..2188 /gene="bla" /label=AmpR promoter promoter 2190..2547 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2213..2233) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2398..2533 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2460..2479 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2582..3376 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2636..2655) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3246..3265 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3564..3583) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3608..3655 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 3984..4572 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4473..4492 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa 61 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 121 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 181 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 241 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt 301 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg 361 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg 421 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat 481 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca 541 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg 601 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc 661 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc 721 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tgacctacgg cgtgcagtgc 781 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa 841 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc 901 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc 961 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaaggtc 1021 tatatcaccg ccgacaagca gaagaacggc atcaaggtga acttcaagac ccgccacaac 1081 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac 1141 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac 1201 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact 1261 ctcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgaattct 1321 gcagtcgacg gtaccgcggg cccgggatcc accggatcta gataactgat cataatcagc 1381 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac 1441 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt 1501 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 1561 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat 1621 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga 1681 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc 1741 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac 1801 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc 1861 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg 1921 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag 1981 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc 2041 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 2101 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 2161 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg 2221 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 2281 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt 2341 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 2401 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 2461 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 2521 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg 2581 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 2641 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 2701 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 2761 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 2821 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 2881 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 2941 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 3001 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 3061 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga 3121 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 3181 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 3241 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 3301 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 3361 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac 3421 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 3481 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 3541 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc 3601 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa 3661 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc 3721 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca 3781 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata 3841 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 3901 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 3961 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 4021 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 4081 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 4141 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 4201 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 4261 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 4321 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 4381 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 4441 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 4501 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 4561 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 4621 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 4681 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc c //