LOCUS Exported 3798 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway Donor vector containing DARC, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.. ACCESSION . VERSION . KEYWORDS pDONR223_DARC_WT_V5 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3798) AUTHORS Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS TITLE High-throughput Phenotyping of Lung Cancer Somatic Mutations. JOURNAL Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28. PUBMED 27478040 REFERENCE 2 (bases 1 to 3798) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3798 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 705..721 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 705..721 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 726..745 /label=T7 /note="T7 promoter, forward primer" promoter 726..744 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 762..861 /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1870..1969) /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1985..2002) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(1985..2001) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1994..2016) /label=M13/pUC Forward /note="In lacZ gene" terminator 2065..2151 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 2243..2270 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" primer_bind complement(2429..2446) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2600..3188) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2680..2699) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3281..274) /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" ORIGIN 1 caacaatggt gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt 61 ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa 121 ccagcaaatc aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat 181 gtacggccag caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt 241 gagtcgatac ttcggcgatc accgcttccc tcataatgtt taactttgtt ttagggcgac 301 tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac 361 gcgcttgctg cttggatgcc cgaggcatag actgtacccc aaaaaaacag tcataacaag 421 ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt 481 tgcgtgagcg catacgctac ttgcattaca gcttacgaac cgaacaggct tatgtccact 541 gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg ggtagcagcg 601 aagtcgaggc atttctgtcc tggctggtct agaggaggca tgatgatata tttttatctt 661 gtgcaatgta acatcagaga ttttgagaca cgggccagag ctgccaggaa acagctatga 721 ccatgtaata cgactcacta taggggatat cagctggatg gcaaataatg attttatttt 781 gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttctt ataatgccaa 841 ctttgtacaa gaaagttggg taggatttgc ttccaagggt gtccagatga gaaaaccatc 901 cttcagggag gggcagagag ggcaagaggg tgcgggtggc ctggtggcag aatagggcga 961 ggagcagggg cgtagccaca cagtgcaaaa ttgccagggc ttctgccagg ttcagcagca 1021 ggtccagagc ctgctgggcc agacatgttg acaacagcaa cagcttggac ctcaccagga 1081 aatccagtcc tagaaccacc ccatgaggcc accagaaaat aaaccaggcc cacaggatgt 1141 tcatccaggg gcctggcccc atacccaatg ccttcttcag ccccttggct ccaaacaaac 1201 ccaatggcaa caagacaaag atggcaagac aggctacagt gtgtgtggcc tgcaaagcct 1261 tcagctccgt gctgtatatc agggtgcaga gtccaccaga agcaccactg gccagggtga 1321 caggcagtgt cagtagggca gccactcccc aaattcccac agtgagcccc agggtgaggc 1381 ctgggacctg gcctgcaccc agtctgtggc ccagggaggc atggcaccct agcagcaaag 1441 cctgggcaaa ggctgagcca taccagacac agtagcccag gctacacagg gcagagctgc 1501 gagtgctacc tagccctggg gccaagacgg gcaccacaat gctgaagagg gcactgccca 1561 cagccagctg tgccaggaca ggccagccag ggcagagctg ccagcggaag agaggtctga 1621 aaagcatgaa gaggacagtg ctgctagcta ggatacccag gacactggtg aggatgaaga 1681 agggcagtgc agagtcatcc agcaggttac aggagtggca gggggcagct gcttccaggt 1741 tggcaccata gtctccatct gggaaggaat cattcacacc ataggaagaa ttccatacat 1801 cttcgaagtc cagctgactt gagttctcag ttgaggggga gagctccgcc ctgtgcagac 1861 agttccccat gccaactttt ttgtacaaag ttggcattat aaaaaagcat tgctcatcaa 1921 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgg ggcccgagct 1981 taagactggc cgtcgtttta caacgtcgtg actgggaaaa catccatgct agcgttaacg 2041 cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgga agactgggcc 2101 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga 2161 gcggatttga acgttgtgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa 2221 actgccaggc atcaaactaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta 2281 caaactcttc ctggctagcg gtacgcgtat taattgcgtt gcgctcactg cccgctttcc 2341 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 2401 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 2461 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 2521 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 2581 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 2641 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 2701 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 2761 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 2821 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 2881 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 2941 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 3001 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 3061 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 3121 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 3181 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgacgcgt 3241 aactcacgtt aagggatttt ggtcatgggt ggctcgaggg ttatttgccg actaccttgg 3301 tgatctcgcc tttcacgtag tggacaaatt cttccaactg atctgcgcgc gaggccaagc 3361 gatcttcttc ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg 3421 ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta 3481 ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt 3541 cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag 3601 gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg 3661 cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa 3721 gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg 3781 gaatgatgtc gtcgtgca //