LOCUS       Exported                1039 bp ds-DNA     linear   SYN 27-JUL-2021
DEFINITION  Insert is a fragment of the Bmpr2 3'-UTR that contains miR-17 and 
            miR-19 binding sites. To be used for 3'UTR assay..
ACCESSION   .
VERSION     .
KEYWORDS    pmirGLO-Bmpr2-1719
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Murphy BL, Obad S, Bihannic L, Ayrault O, Zindy F, Kauppinen S, 
            Roussel MF
  TITLE     Silencing of the miR-17~92 cluster family inhibits medulloblastoma 
            progression.
  JOURNAL   Cancer Res. 2013 Oct 21.
  PUBMED    24145352
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 27, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(84..1016)
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     regulatory      1013..1022
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 ggagaacctg cgtgcaatcc atcttgttca atccctccgg agcctccgcc acctgatccg
       61 ccacctccgc tcccaccacc ggtctgctcg ttcttcagca cgcgctccac gaagctcttg
      121 atgtacttac ccatttcatc tggagcgtcc tcctggctga agtggaggcc cttcaccttc
      181 acgaactcgg tgttagggaa cttcttagct ccctcgacaa tagcgttgga aaagaaccca
      241 gggtcggact cgatgaacat cttaggcaga tcgtcgctgg cccgaaggta ggcgttgtag
      301 ttgcggacaa tctggacgac gtcgggcttg cctcccttaa cgagagggat ctcgcgaggc
      361 caggagaggg taggccgtct aacctcgccc ttctccttga atggctccag gtaggcagcg
      421 aactcctcag gctccagttt ccgcatgatc ttgcttggga gcatggtctc gacgaagaag
      481 ttattctcaa gcaccatttt ctcgccctct tcgctcttga tcagggcgat atcctcctcg
      541 atgtcaggcc actcgtccca ggactcgatc acgtccacga cactctcagc atggacgatg
      601 gccttgatct tgtcttggtg ctcgtaggag tagtgaaagg ccagacaagc cccccagtcg
      661 tggcccacaa agatgatttt ctttggaagg ttcagcagct cgaaccaagc ggtgaggtac
      721 ttgtagtgat ccaggaggcg atatgagcca ttcccgctct tgccggactt acccattccg
      781 atcagatcag ggatgatgca tctagccacg ggctcgatgt gaggcacgac gtgcctccac
      841 aggtagctgg aggcagcgtt accatgcaga aaaatcacgg cgttctcggc gtgcttctcg
      901 gaatcatagt agttgatgaa ggagtccagc acgttcattt gcttgcagcg agcccaccac
      961 tgaggcccag tgatcatgcg tttgcgttgc tcggggtcgt acaccttgga agccatggtg
     1021 gctccagcct ttggttcga
//
