LOCUS       Exported                 960 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  Cre recombinase-dependent GFP expression in forebrain GABA-ergic 
            interneurons under the control of the hDlx enhancer element.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-hDlx-Flex-GFP-Fishell_6
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Dimidschstein J, Chen Q, Tremblay R, Rogers SL, Saldi GA, Guo L, Xu 
            Q, Liu R, Lu C, Chu J, Avery MC, Rashid MS, Baek M, Jacob AL, Smith 
            GB, Wilson DE, Kosche G, Kruglikov I, Rusielewicz T, Kotak VC, 
            Mowery TM, Anderson SA, Callaway EM, Dasen JS, Fitzpatrick D, 
            Fossati V, Long MA, Noggle S, Reynolds JH, Sanes DH, Rudy B, Feng G,
            Fishell G
  TITLE     A viral strategy for targeting and manipulating interneurons across 
            vertebrate species.
  JOURNAL   Nat Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430.
  PUBMED    27798629
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(9..42)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    complement(93..126)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     primer_bind     complement(174..193)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          176..308
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 ggcgcgccat aacttcgtat aatgtatgct atacgaagtt attaagaggt ttcatattgc
       61 taatagcagc tacaatccag ctaccattct gcataacttc gtataaagta tcctatacga
      121 agttattccg gagtcgacct ttcttaagag ctgtaattga actgggagtg gacacctgtg
      181 gagagaaagg caaagtggat gtcagtaaga ccaataggtg cctatcagaa acgcaagagt
      241 cttctctgtc tcgacaagcc cagtttctat tggtctcctt aaacctgtct tgtaaccttg
      301 atacttacct gcccagtgcc tcacgaccaa cttctgcagc ttaaggcgcc gcgctctgct
      361 tctggaaggc tgcgctcctc gacctgcagg ctagaagcaa atgtaagcaa tagatggctc
      421 tgccctgact tttatgccca gcccgggctg caggaattcg atatcaagct tcctactgtg
      481 aaactttggg ttcgtagctc cccaggatca attctgaaca aagcctccag ctgcagtgcc
      541 atccaatttg aagcagacat tggggacaat ttaaggtttt tatccacaag aaggtttttt
      601 tccattctct taaatgcagc cataattaga gtaatttttc atgtagcccg ctgattacag
      661 cgtttttacc gtcaaagata attacctgta attttcttcc acttttaata ctaaaaagcc
      721 atctttattt agattcagga acaggaaagg cgaaacaaaa gagggaaatt attctgttat
      781 tcatacacaa attgcagaga cgtaggacct aaaattgaaa attaaccaaa attataatgc
      841 tgaaaagaat ggaagaggct gcagaagtac agctggtagt ggggctttgc tgaattattc
      901 aaattacgtt agggagaatg ctaccataca tcgaaaccaa cactaatttt tcgttaaaaa
//
