LOCUS       Exported                6666 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Gateway Donor vector containing PER1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    pDONR223_PER1_WT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6666)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 6666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6666
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4066..4165)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4182..4201)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4183..4201)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4206..4222)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4206..4222)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4653..5444
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5537..6125
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6026..6045
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6279..6296
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      6455..6482
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      6574..6660
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgagtggc cccctagaag gggctgatgg gggaggggac cccaggcctg
      241 gggaatcatt ttgtcctggg ggcgtcccat cccctgggcc cccacagcac cggccttgcc
      301 caggccccag cctggccgat gacaccgatg ccaacagcaa tggttcaagt ggcaatgagt
      361 ccaacgggca tgagtctaga ggcgcatctc agcggagctc acacagctcc tcctcaggca
      421 acggcaagga ctcagccctg ctggagacca ctgagagcag caagagcaca aactctcaga
      481 gcccatcccc acccagcagt tccattgcct acagcctcct gagtgccagc tcagagcagg
      541 acaacccgtc caccagtggc tgcagcagtg aacagtcagc ccgggcaagg actcagaagg
      601 aactcatgac agcacttcga gagctcaagc ttcgactgcc gccagagcgc cggggcaagg
      661 gccgctctgg gaccctggcc acgctgcagt acgcactggc ctgtgtcaag caggtgcagg
      721 ccaaccagga atactaccag cagtggagcc tggaggaggg cgagccttgc tccatggaca
      781 tgtccaccta taccctggag gagctggagc acatcacgtc tgagtacaca cttcagaacc
      841 aggatacctt ctcagtggct gtctccttcc tgacgggccg aatcgtctac atttcggagc
      901 aggcagccgt cctgctgcgt tgcaagcggg acgtgttccg gggtacccgc ttctctgagc
      961 tcctggctcc ccaggatgtg ggagtcttct atggttccac tgctccatct cgcctgccca
     1021 cctggggcac aggggcctca gcaggttcag gcctcaggga ctttacccag gagaagtccg
     1081 tcttctgccg tatcagagga ggtcctgacc gggatccagg gcctcggtac cagccattcc
     1141 gcctaacccc gtatgtgacc aagatccggg tctcagatgg ggcccctgca cagccgtgct
     1201 gcctgctgat tgcagagcgc atccattcgg gttacgaagc tccccggata ccccctgaca
     1261 agaggatttt cactacgcgg cacacaccca gctgcctctt ccaggatgtg gatgaaaggg
     1321 ctgcccccct gctgggctac ctgccccagg acctcctggg ggccccagtg ctcctgttcc
     1381 tgcatcctga ggaccgaccc ctcatgctgg ctatccacaa gaagattctg cagttggcgg
     1441 gccagccctt tgaccactcc cctatccgct tctgtgcccg caacggggag tatgtcacca
     1501 tggacaccag ctgggctggc tttgtgcacc cctggagccg caaggtagcc ttcgtgttgg
     1561 gccgccacaa agtacgcacg gcccccctga atgaggacgt gttcactccc ccggccccca
     1621 gcccagctcc ctccctggac actgatatcc aggagctgtc agagcagatc caccggctgc
     1681 tgctgcagcc cgtccacagc cccagcccca cgggactctg tggagtcggc gccgtgacat
     1741 ccccaggccc tctccacagc cctgggtcct ccagtgatag caacgggggt gatgcagagg
     1801 ggcctgggcc tcctgcgcca gtgactttcc agcagatctg taaggatgtg catctggtga
     1861 agcaccaggg ccagcagctt tttattgagt ctcgggcccg gcctcagtcc cggccccgcc
     1921 tccctgctac aggcacgttc aaggccaagg cccttccctg ccaatcccca gacccagagc
     1981 tggaggcggg ttctgctccc gtccaggccc cactagcctt ggtccctgag gaggccgaga
     2041 ggaaagaagc ctccagctgc tcctaccagc agatcaactg cctggacagc atcctcaggt
     2101 acctggagag ctgcaacctc cccagcacca ctaagcgtaa atgtgcctcc tcctcctcct
     2161 ataccacctc ctcagcctct gacgacgaca ggcagaggac aggtccagtc tctgtgggga
     2221 ccaagaaaga tccgccgtca gcagcgctgt ctggggaggg ggccacccca cggaaggagc
     2281 cagtggtggg aggcaccctg agcccgctcg ccctggccaa taaggcggag agtgtggtgt
     2341 ccgtcaccag tcagtgtagc ttcagctcca ccatcgtcca tgtgggagac aagaagcccc
     2401 cggagtcgga catcatcatg atggaggacc tgcctggtct agccccaggc ccagccccca
     2461 gcccagcccc cagccccaca gtagcccctg acccagcccc agacgcctac cgtccagtgg
     2521 ggctgaccaa ggccgtgctg tccctgcaca cgcagaagga agagcaagcc ttcctcagcc
     2581 gcttccgaga cctgggcagg ctgcgtggac tcgacagctc ttccacagct ccctcagccc
     2641 ttggcgagcg aggctgccac cacggccccg cacccccaag ccgccgacac cactgccgat
     2701 ccaaagccaa gcgctcacgc caccaccaga accctcgggc tgaagcgccc tgctatgtct
     2761 cacacccctc acccgtgcca ccctccaccc cctggcccac cccaccagcc actaccccct
     2821 tcccagcggt tgtccagccc taccctctcc cagtgttctc tcctcgagga ggcccccagc
     2881 ctcttccccc tgctcccaca tccgtgcccc cagctgcttt ccccgcccct ttggtgaccc
     2941 caatggtggc cttggtgctc cctaactatc tgttcccaac cccatccagc tatccttatg
     3001 gggcactcca gacccctgct gaagggcctc ccactcctgc ctcgcactcc ccttctccat
     3061 ccttgcccgc cctccccccg agtcctcctc accgcccgga ctctccactg ttcaactcga
     3121 gatgcagctc tccactccag ctcaatctgc tgcagctgga ggagctcccc cgtgctgagg
     3181 gggctgctgt tgcaggaggc cctgggagca gtgccgggcc cccacctccc agtgcggagg
     3241 ctgctgagcc agaggccaga ctggcggagg tcactgagtc ctccaatcag gacgcacttt
     3301 ccggctccag tgacctgctc gaacttctgc tgcaagagga ctcgcgctcc ggcacaggct
     3361 ccgcagcctc gggctccttg ggctctggct tgggctctgg gtctggttca ggctcccatg
     3421 aagggggcag cacctcagcc agcatcactc gcagcagcca gagcagccac acaagcaaat
     3481 actttggcag catcgactct tccgaggctg aggctggggc tgctcggggc ggggctgagc
     3541 ctggggacca ggtgattaag tacgtgctcc aggatcccat ttggctgctc atggccaatg
     3601 ctgaccagcg cgtcatgatg acctaccagg tgccctccag ggacatgacc tctgtgctga
     3661 agcaggatcg ggagcggctc cgagccatgc agaagcagca gcctcggttt tctgaggacc
     3721 agcggcggga actgggtgct gtgcactcct gggtccggaa gggccaactg cctcgggctc
     3781 ttgatgtgat ggcctgtgtg gactgtggga gcagcaccca agatcctggt caccctgatg
     3841 acccactctt ctcagagctg gatggactgg ggctggagcc catggaagag ggtggaggcg
     3901 agcagggcag cagcggtggc ggcagtggtg agggagaggg ctgcgaggag gcccaaggcg
     3961 gggccaaggc ttcaagctct caggacttgg ctatggagga ggaggaagaa ggcaggagct
     4021 catccagtcc agccttacct acagcaggaa actgcaccag ctagttgcca actttcttgt
     4081 acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta
     4141 tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt
     4201 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
     4261 ttgcacaaga taaaaatata tcatcatgcc tcctctagac cagccaggac agaaatgcct
     4321 cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac
     4381 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
     4441 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     4501 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     4561 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     4621 agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat
     4681 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     4741 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
     4801 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     4861 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     4921 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     4981 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     5041 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     5101 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     5161 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     5221 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     5281 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     5341 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     5401 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat
     5461 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     5521 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5581 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5641 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     5701 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5761 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5821 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5881 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     5941 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6001 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6061 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6121 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6181 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     6241 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     6301 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     6361 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc
     6421 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     6481 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     6541 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     6601 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg
     6661 atgcct
//
