LOCUS Exported 4998 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.. ACCESSION . VERSION . KEYWORDS NPY-pHTomato SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4998) AUTHORS Gandasi NR, Vesto K, Helou M, Yin P, Saras J, Barg S TITLE Survey of Red Fluorescence Proteins as Markers for Secretory Granule Exocytosis. JOURNAL PLoS One. 2015 Jun 19;10(6):e0127801. doi: 10.1371/journal.pone.0127801. eCollection 2015. PUBMED 26091288 REFERENCE 2 (bases 1 to 4998) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4998 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 519..539 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind complement(1098..1116) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 1366..1385 /label=mCherry-F /note="mCherry, forward primer" primer_bind 1560..1583 /label=DsRed1-C /note="DsRed1, forward primer" polyA_signal 1786..1907 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1823..1842) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1877..1896 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1914..2369) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2051..2072) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2263..2282 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2396..2500 /gene="bla" /label=AmpR promoter promoter 2502..2859 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2525..2545) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2710..2845 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2772..2791 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2894..3688 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2948..2967) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3558..3577 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3876..3895) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3920..3967 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4296..4884 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4785..4804 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttagc caccatgcta ggtaacaagc gactggggct 661 gtccggactg accctcgccc tgtccctgct cgtgtgcctg ggtgcgctgg ccgaggcgta 721 cccctccaag ccggacaacc cgggcgagga cgcaccagcg gaggacatgg ccagatacta 781 ctcggcgctg cgacactaca tcaacctcat caccaggcag agatatggaa aacgatccag 841 cccagagaca ctgatttcag acctcttgat gagagaaagc acagaaaatg ttcccagaac 901 tcggcttgaa gaccctgcaa tgtggacggt accgcgggcc cgggatccac cggtcgtgag 961 caagggcgag gagaataaca tggccatcat caaggagttc atgcgcttca aggtgcgcat 1021 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta 1081 cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg 1141 ggacatcctg tcccctcagt tcacgtacgg ctccaaggcc tacgtgaagc accccgccga 1201 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa 1261 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt 1321 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa 1381 gaagaccatg ggctgggagg cctccaccga gcggatgtac cccgaggacg gcgccctgaa 1441 gggcgagatc aagatgaggc tgaagctgaa ggacggcggc cactacgacg ccgaggtcaa 1501 gaccacctac atggccaaga agcccgtgca gctgcccggc gcctacaaga ccgacatcaa 1561 gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg agcgcgccga 1621 gggccgccac tccaccggcg gcatggacga gctgtacaag taaagcggcc gcgactctag 1681 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac 1741 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca 1801 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 1861 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt 1921 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa 1981 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt 2041 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa 2101 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag 2161 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt 2221 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg 2281 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc 2341 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg 2401 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 2461 accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag 2521 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 2581 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 2641 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 2701 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 2761 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 2821 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg 2881 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt 2941 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt 3001 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc 3061 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc 3121 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga 3181 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat 3241 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca 3301 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga 3361 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc 3421 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat 3481 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga 3541 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg 3601 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt 3661 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa 3721 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg 3781 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg 3841 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg 3901 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg 3961 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga 4021 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg 4081 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt 4141 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg 4201 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 4261 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 4321 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 4381 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 4441 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 4501 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 4561 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 4621 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 4681 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 4741 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 4801 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 4861 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 4921 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 4981 cgtattaccg ccatgcat //