LOCUS Exported 7123 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Retroviral expression of dominant active mouse Stat3. ACCESSION . VERSION . KEYWORDS pMXs-Stat3-C SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7123) AUTHORS Takahashi K, Yamanaka S TITLE Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. JOURNAL Cell. 2006 Aug 25. 126(4):663-76. PUBMED 16904174 REFERENCE 2 (bases 1 to 7123) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7123 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(2313..2337) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" LTR 2654..3123 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(3253..3270) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3424..4012) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3504..3523) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4176..5036) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4799..4818 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5037..5141) /gene="bla" /label=AmpR promoter LTR 5171..5764 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 5827..6184 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 6243..6659 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 6589..6611 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 6627..6643 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" misc_feature 6669..7043 /label=pol region /note="Moloney murine leukemia virus (MMLV) pol region containing the splice acceptor site" primer_bind 6981..6997 /label=pBMN 5' /note="MMLV sequence, for inserts in pBMN retroviral vector" protein_bind 7094..7118 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" ORIGIN 1 atggctcagt ggaaccagct gcagcagctg gacacacgct acctggagca gctgcaccag 61 ctgtacagcg acagcttccc catggagctg cggcagttcc tggcaccttg gattgagagt 121 caagactggg catatgcagc cagcaaagag tcacatgcca cgttggtgtt tcataatctc 181 ttgggtgaaa ttgaccagca atatagccga ttcctgcaag agtccaatgt cctctatcag 241 cacaaccttc gaagaatcaa gcagtttctg cagagcaggt atcttgagaa gccaatggaa 301 attgcccgga tcgtggcccg atgcctgtgg gaagagtctc gcctcctcca gacggcagcc 361 acggcagccc agcaaggggg ccaggccaac cacccaacag ccgccgtagt gacagagaag 421 cagcagatgt tggagcagca tcttcaggat gtccggaagc gagtgcagga tctagaacag 481 aaaatgaagg tggtggagaa cctccaggac gactttgatt tcaactacaa aaccctcaag 541 agccaaggag acatgcagga tctgaatgga aacaaccagt ctgtgaccag acagaagatg 601 cagcagctgg aacagatgct cacagccctg gaccagatgc ggagaagcat tgtgagtgag 661 ctggcggggc tcttgtcagc aatggagtac gtgcagaaga cactgactga tgaagagctg 721 gctgactgga agaggcggca gcagatcgcg tgcatcggag gccctcccaa catctgcctg 781 gaccgtctgg aaaactggat aacttcatta gcagaatctc aacttcagac ccgccaacaa 841 attaagaaac tggaggagct gcagcagaaa gtgtcctaca agggcgaccc tatcgtgcag 901 caccggccca tgctggagga gaggatcgtg gagctgttca gaaacttaat gaagagtgcc 961 ttcgtggtgg agcggcagcc ctgcatgccc atgcacccgg accggccctt agtcatcaag 1021 actggtgtcc agtttaccac gaaagtcagg ttgctggtca aatttcctga gttgaattat 1081 cagcttaaaa ttaaagtgtg cattgataaa gactctgggg atgttgctgc cctcagaggg 1141 tctcggaaat ttaacattct gggcacgaac acaaaagtga tgaacatgga ggagtctaac 1201 aacggcagcc tgtctgcaga gttcaagcac ctgaccctta gggagcagag atgtgggaat 1261 ggaggccgtg ccaattgtga tgcctccttg atcgtgactg aggagctgca cctgatcacc 1321 ttcgagactg aggtgtacca ccaaggcctc aagattgacc tagagaccca ctccttgcca 1381 gttgtggtga tctccaacat ctgtcagatg ccaaatgctt gggcatcaat cctgtggtat 1441 aacatgctga ccaataaccc caagaacgtg aacttcttca ctaagccgcc aattggaacc 1501 tgggaccaag tggccgaggt gctcagctgg cagttctcgt ccaccaccaa gcgggggctg 1561 agcatcgagc agctgacaac gctggctgag aagctcctag ggcctggtgt gaactactca 1621 gggtgtcaga tcacatgggc taaattctgc aaagaaaaca tggctggcaa gggcttctcc 1681 ttctgggtct ggctagacaa tatcatcgac cttgtgaaaa agtatatctt ggccctttgg 1741 aatgaagggt acatcatggg tttcatcagc aaggagcggg agcgggccat cctaagcaca 1801 aagcccccgg gcaccttcct actgcgcttc agcgagagca gcaaagaagg aggggtcact 1861 ttcacttggg tggaaaagga catcagtggc aagacccaga tccagtctgt agagccatac 1921 accaagcagc agctgaacaa catgtcattt gctgaaatca tcatgggcta taagatcatg 1981 gattgtacct gcatcctggt gtctccactt gtctacctct accccgacat tcccaaggag 2041 gaggcatttg gaaagtactg taggcccgag agccaggagc accccgaagc cgacccaggt 2101 agtgctgccc cgtacctgaa gaccaagttc atctgtgtga caccaacgac ctgcagcaat 2161 accattgacc tgccgatgtc cccccgcact ttagattcat tgatgcagtt tggaaataac 2221 ggtgaaggtg ctgagccctc agcaggaggg cagtttgagt cgctcacgtt tgacatggat 2281 ctgacctcgg agtgtgctac ctcccccatg tgacccagct ttcttgtaca aagtggtgat 2341 attccagctg agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataataata 2401 accgggcagg ccatgtctgc ccgtatttcg cgtaaggaaa tccattatgt actatttaaa 2461 ctcgagcggc cgccagcaca gtggtcgacg ataaaataaa agattttatt tagtctccag 2521 aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca 2581 ttttgcaagg catggaaaaa tacataactg agaatagaga agttcagatc aaggtcagga 2641 acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc 2701 ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat atctgtggta 2761 agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg gtccagccct 2821 cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc 2881 tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct 2941 ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc tccgattgac 3001 tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg 3061 tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt 3121 tcacatgcag catgtatcaa aattaatttg gttttttttc ttaagtattt acattaaatg 3181 gccatagttg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 3241 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 3301 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 3361 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 3421 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 3481 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 3541 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 3601 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 3661 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 3721 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 3781 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 3841 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 3901 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 3961 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 4021 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 4081 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttgcggccgg ccgcaaatca 4141 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 4201 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 4261 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 4321 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 4381 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 4441 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 4501 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 4561 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 4621 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 4681 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 4741 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 4801 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 4861 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 4921 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 4981 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 5041 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 5101 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 5161 ccacctgcat aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat 5221 tttgcaaggc atggaaaaat acataactga gaatagaaaa gttcagatca aggtcaggaa 5281 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg 5341 gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa 5401 gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc 5461 agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct 5521 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc 5581 cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgattgact 5641 gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt 5701 ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt 5761 catttggggg ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg 5821 ggaggtaagc tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgactga 5881 ttttatgcgc ctgcgtcggt actagttagc taactagctc tgtatctggc ggacccgtgg 5941 tggaactgac gagttcggaa cacccggccg caaccctggg agacgtccca gggacttcgg 6001 gggccgtttt tgtggcccga cctgagtcca aaaatcccga tcgttttgga ctctttggtg 6061 caccccccta ataggaggga tatgtggttc tggtaggaga cgagaaccta aaacagttcc 6121 cgcctccgtc tgaatttttg ctttcggttt gggaccgaag ccgcgccgcg cgtcttgtct 6181 gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt 6241 agggccagac tgttaccact cccttaagtt tgaccttagg tcactggaaa gatgtcgagc 6301 ggatcgctca caaccagtcg gtagatgtca agaagagacg ttgggttacc ttctgctctg 6361 cagaatggcc aacctttaac gtcggatggc cgcgagacgg cacctttaac cgagacctca 6421 tcacccaggt taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc 6481 cctacatcgt gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg 6541 tacaccctaa gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc 6601 ctcgttcgac cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccc 6661 ccatatggcc atatgagatc ttatatgggg cacccccgcc ccttgtaaac ttccctgacc 6721 ctgacatgac aagagttact aacagcccct ctctccaagc tcacttacag gctctctact 6781 tagtccagca cgaagtctgg agacctctgg cggcagccta ccaagaacaa ctggaccgac 6841 cggtggtacc tcacccttac cgagtcggcg acacagtgtg ggtccgccga caccagacta 6901 agaacctaga acctcgctgg aaaggacctt acacagtcct gctgaccacc cccaccgccc 6961 tcaaagtaga cggcatcgca gcttggatac acgccgccca cgtgaaggct gccgaccccg 7021 ggggtggacc atcctctaga ctgccggatc tagctagtta attaaggatc ccagtgtggt 7081 ggtacgggaa atcacaagtt tgtacaaaaa agcaggctcc agg //