LOCUS Exported 2325 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Encodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter. . ACCESSION . VERSION . KEYWORDS pICSL01009::AtU6p SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2325) AUTHORS Nekrasov V, Staskawicz B, Weigel D, Jones JD, Kamoun S TITLE Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease. JOURNAL Nat Biotechnol. 2013 Aug 8;31(8):691-3. doi: 10.1038/nbt.2655. PUBMED 23929340 REFERENCE 2 (bases 1 to 2325) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2325 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 27..131 /gene="bla" /label=AmpR promoter CDS 353..1144 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 1315..1903 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1804..1823 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2057..2074 /label=L4440 /note="L4440 vector, forward primer" promoter 2155..2229 /label=AtU6 promoter /note="consensus promoter from the three Arabidopsis U6 snRNA genes (Waibel and Filipowicz, 1990; Nekrasov et al., 2013)" primer_bind complement(2266..2284) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta 181 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttttgggg tacagtctat 241 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag 301 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga 361 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca 421 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa 481 gccacacagc gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg 541 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct 601 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc 661 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga 721 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt 781 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt 841 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga 901 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc 961 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt 1021 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc 1081 agatcagttg gaagaatttg tccattacgt aaaaggcgag atcaccaagg tagtcggcaa 1141 ataactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 1201 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 1261 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 1321 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 1381 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 1441 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa 1501 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 1561 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 1621 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 1681 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 1741 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 1801 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 1861 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 1921 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 1981 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag 2041 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa 2101 accgcctctc cccgcgcgtt ggccgattca ttaatcactc tgtggtctca ggagtgatca 2161 aaagtcccac atcgatcagg tgatatatag cagcttagtt tatataatga tagagtcgac 2221 atagcgattg tgagaccacg aagtggctct tcagtggacg aaagggcctc gtgatacgcc 2281 tatttttata ggttaatgtc atgataataa tggtttctta gacgt //