LOCUS Exported 5075 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION In vivo visualization of the nucleus (can be used for colocalization studies). ACCESSION . VERSION . KEYWORDS pmScarlet_H2A_C1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5075) AUTHORS Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M, Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr TITLE mScarlet: a bright monomeric red fluorescent protein for cellular imaging. JOURNAL Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074. PUBMED 27869816 REFERENCE 2 (bases 1 to 5075) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5075 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 158..177 /label=F1ori-R /note="F1 origin, reverse primer" promoter 291..395 /gene="bla" /label=AmpR promoter promoter 397..754 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(420..440) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 605..740 /label=SV40 ori /note="SV40 origin of replication" primer_bind 667..686 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 789..1583 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(843..862) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 1453..1472 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(1771..1790) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 1815..1862 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 2191..2779 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2680..2699 /label=pBR322ori-F /note="pBR322 origin, forward primer" enhancer 2954..3257 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 3258..3461 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 3412..3432 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 3500..3509 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3506..4201 /codon_start=1 /product="bright monomeric red fluorescent protein evolved from a synthetic template (Bindels et al., 2016)" /label=mScarlet /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH STGGMDELYK" primer_bind complement(3639..3657) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 3907..3926 /label=mCherry-F /note="mCherry, forward primer" polyA_signal 4756..4877 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4793..4812) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4847..4866 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(4884..264) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5021..5042) /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt 61 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag 121 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg 181 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc 241 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 301 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 361 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg 421 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 481 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg 541 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 601 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 661 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg 721 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat 781 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga 841 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc 901 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga 961 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg 1021 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc 1081 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg 1141 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga 1201 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc 1261 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca 1321 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg 1381 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct 1441 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg 1501 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc 1561 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac 1621 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt 1681 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga 1741 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg 1801 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt 1861 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca 1921 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga 1981 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc 2041 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 2101 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 2161 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 2221 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 2281 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct 2341 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 2401 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 2461 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 2521 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 2581 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 2641 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 2701 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 2761 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 2821 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 2881 taccgccatg cattagttat taatagtaat caattacggg gtcattagtt catagcccat 2941 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 3001 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 3061 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 3121 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 3181 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 3241 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 3301 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 3361 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 3421 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga 3481 tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggcagtga tcaaggagtt 3541 catgcggttc aaggtgcaca tggagggctc catgaacggc cacgagttcg agatcgaggg 3601 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg 3661 tggccccctg cccttctcct gggacatcct gtcccctcag ttcatgtacg gctccagggc 3721 cttcaccaag caccccgccg acatccccga ctactataag cagtccttcc ccgagggctt 3781 caagtgggag cgcgtgatga acttcgagga cggcggcgcc gtgaccgtga cccaggacac 3841 ctccctggag gacggcaccc tgatctacaa ggtgaagctc cgcggcacca acttccctcc 3901 tgacggcccc gtaatgcaga agaagacaat gggctgggaa gcatccaccg agcggttgta 3961 ccccgaggac ggcgtgctga agggcgacat taagatggcc ctgcgcctga aggacggcgg 4021 ccgctacctg gcggacttca agaccaccta caaggccaag aagcccgtgc agatgcccgg 4081 cgcctacaac gtcgaccgca agttggacat cacctcccac aacgaggact acaccgtggt 4141 ggaacagtac gaacgctccg agggccgcca ctccaccggc ggcatggacg agctgtacaa 4201 gtactcagat ctcgagctca agcttcgaat tctgatgtcg ggacgcggca agcagggagg 4261 caaagctcgc gccaaagcca agacccgctc ttctcgtgcc ggtctccagt tccccgtggg 4321 ccgagtgcac cgactgctcc gcaagggcaa ctatgctgag cgggtcgggg ccggcgcgcc 4381 ggtgtacctg gcggcggtgc tggagtacct gactgccgag atcctggagc tggcgggcaa 4441 cgccgcccgc gacaacaaga agacccgcat tatcccgcgc cacttgcagc tggccatccg 4501 caacgacgag gagctcaaca agctgctggg caaagtaacc atcgctcagg gtggtgtcct 4561 gcccaacatc caggctgtgc tactgcccaa gaagaccgag agtcaccaca aggccaaagg 4621 caaacgggat ccaccggatc tagataactg atcataatca gccataccac atttgtagag 4681 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat 4741 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc 4801 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 4861 ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt 4921 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta 4981 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc 5041 actattaaag aacgtggact ccaacgtcaa agggc //