LOCUS Exported 4826 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION In vivo visualization of the mitochondria (can be used for colocalization studies). ACCESSION . VERSION . KEYWORDS pMTS_mScarlet_N1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4826) AUTHORS Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M, Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr TITLE mScarlet: a bright monomeric red fluorescent protein for cellular imaging. JOURNAL Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074. PUBMED 27869816 REFERENCE 2 (bases 1 to 4826) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4826 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 85..388 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 389..592 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 543..563 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 621..695 /codon_start=1 /product="mitochondrial presequence of human cytochrome c oxidase subunit VIII (Rizutto et al., 1989)" /label=COX8 presequence /translation="MSVLTPLLLRGLTGSARRLPVPRAK" regulatory 723..732 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 729..1424 /codon_start=1 /product="bright monomeric red fluorescent protein evolved from a synthetic template (Bindels et al., 2016)" /label=mScarlet /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH STGGMDELYK" primer_bind complement(862..880) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 1130..1149 /label=mCherry-F /note="mCherry, forward primer" misc_feature 1425..1490 /label=MCS /note="multiple cloning site" polyA_signal 1614..1735 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1651..1670) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1705..1724 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1742..2197) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1879..1900) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2091..2110 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2224..2328 /gene="bla" /label=AmpR promoter promoter 2330..2687 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2353..2373) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2538..2673 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2600..2619 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2722..3516 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2776..2795) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3386..3405 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3704..3723) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3748..3795 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4124..4712 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4613..4632 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 atcccgggcc cgcggtaccg tcgatagtta ttaatagtaa tcaattacgg ggtcattagt 61 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 121 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 181 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc 241 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 301 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 361 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg 421 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 481 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt 541 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt 601 gaaccgtcag atccgctagc atgtccgtcc tgacgccgct gctgctgcgg ggcttgacag 661 gctcggcccg gcggctccca gtgccgcgcg ccaagatcca ttcgttgggg gatccaccgg 721 tcgccaccat ggtgagcaag ggcgaggcag tgatcaagga gttcatgcgg ttcaaggtgc 781 acatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc 841 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttct 901 cctgggacat cctgtcccct cagttcatgt acggctccag ggccttcacc aagcaccccg 961 ccgacatccc cgactactat aagcagtcct tccccgaggg cttcaagtgg gagcgcgtga 1021 tgaacttcga ggacggcggc gccgtgaccg tgacccagga cacctccctg gaggacggca 1081 ccctgatcta caaggtgaag ctccgcggca ccaacttccc tcctgacggc cccgtaatgc 1141 agaagaagac aatgggctgg gaagcatcca ccgagcggtt gtaccccgag gacggcgtgc 1201 tgaagggcga cattaagatg gccctgcgcc tgaaggacgg cggccgctac ctggcggact 1261 tcaagaccac ctacaaggcc aagaagcccg tgcagatgcc cggcgcctac aacgtcgacc 1321 gcaagttgga catcacctcc cacaacgagg actacaccgt ggtggaacag tacgaacgct 1381 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtccgga ctcagatctc 1441 gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggatcta 1501 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc 1561 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt 1621 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 1681 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg 1741 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca 1801 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag 1861 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc 1921 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc 1981 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc 2041 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa 2101 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc 2161 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat 2221 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 2281 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa 2341 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 2401 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 2461 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 2521 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 2581 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 2641 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag 2701 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc 2761 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 2821 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 2881 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg 2941 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 3001 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta 3061 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 3121 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 3181 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 3241 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg 3301 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 3361 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 3421 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 3481 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga 3541 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg 3601 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg 3661 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga 3721 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg 3781 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc 3841 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc 3901 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag 3961 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 4021 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 4081 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 4141 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 4201 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 4261 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 4321 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 4381 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 4441 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 4501 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 4561 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 4621 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 4681 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 4741 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 4801 tggataaccg tattaccgcc atgcat //