LOCUS       Exported                3369 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Gateway Donor vector containing CDC42 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    pDONR223_CDC42_p.Q61L
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     429..448
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     682..699
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      858..885
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      977..1063
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1112..1134
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1126..1143
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1127..1143
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1159..1258
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1261..1836
                     /codon_start=1
                     /product="constitutively active mutant of human Rho family 
                     small GTPase Cdc42"
                     /label=Cdc42 (Q61L)
                     /translation="MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVM
                     IGGEPYTLGLFDTAGLEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHH
                     CPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQK
                     GLKNVFDEAILAALEPPEPKKSRRCVLL"
     protein_bind    complement(1838..1937)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1954..1973)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1955..1973)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1978..1994)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1978..1994)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2425..3216
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3309..528
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
       61 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
      121 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      181 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      241 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      301 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      361 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      421 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      481 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
      541 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      601 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
      661 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
      721 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
      781 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
      841 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
      901 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
      961 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     1021 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     1081 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     1141 agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac ctgttcgttg
     1201 caacaaattg atgagcaatg cttttttata atgacaactt tgtacaaaaa agttggcacc
     1261 atgcagacaa ttaagtgtgt tgttgtgggc gatggtgctg ttggtaaaac atgtctcctg
     1321 atatcctaca caacaaacaa atttccatcg gaatatgtac cgactgtttt tgacaactat
     1381 gcagtcacag ttatgattgg tggagaacca tatactcttg gactttttga tactgcaggg
     1441 ctagaggatt atgacagatt acgaccgctg agttatccac aaacagatgt atttctagtc
     1501 tgtttttcag tggtctctcc atcttcattt gaaaacgtga aagaaaagtg ggtgcctgag
     1561 ataactcacc actgtccaaa gactcctttc ttgcttgttg ggactcaaat tgatctcaga
     1621 gatgacccct ctactattga gaaacttgcc aagaacaaac agaagcctat cactccagag
     1681 actgctgaaa agctggcccg tgacctgaag gctgtcaagt atgtggagtg ttctgcactt
     1741 acacagaaag gcctaaagaa tgtatttgac gaagcaatat tggctgccct ggagcctcca
     1801 gaaccgaaga agagccgcag gtgtgtgctg ctatgattgc caactttctt gtacaaagtt
     1861 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     1921 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
     1981 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     2041 gataaaaata tatcatcatg cctcctctag accagccagg acagaaatgc ctcgacttcg
     2101 ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag
     2161 tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac
     2221 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
     2281 tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt
     2341 tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt
     2401 cgccctaaaa caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa
     2461 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
     2521 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt
     2581 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
     2641 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg
     2701 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
     2761 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct
     2821 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     2881 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     2941 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
     3001 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
     3061 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt
     3121 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac
     3181 gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa
     3241 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3301 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3361 ccgctacca
//
