LOCUS Exported 3705 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway Donor vector containing CDK4 , part of the Target Accelerator Plasmid Collection.. ACCESSION . VERSION . KEYWORDS pDONR223_CDK4_p.R24C SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3705) AUTHORS Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn WC TITLE Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. JOURNAL Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4. PUBMED 27147599 REFERENCE 2 (bases 1 to 3705) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3705 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 43..65 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 57..74 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 58..74 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 90..189 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1105..1204) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1221..1240) /label=T7 /note="T7 promoter, forward primer" promoter complement(1222..1240) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1245..1261) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1245..1261) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 1692..2483 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 2576..3164 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3065..3084 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3318..3335 /label=L4440 /note="L4440 vector, forward primer" terminator 3494..3521 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3613..3699 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta 61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga 121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa 181 aagttggcac catggctacc tctcgatatg agccagtggc tgaaattggt gtcggtgcct 241 atgggacagt gtacaaggcc tgtgatcccc acagtggcca ctttgtggcc ctcaagagtg 301 tgagagtccc caatggagga ggaggtggag gaggccttcc catcagcaca gttcgtgagg 361 tggctttact gaggcgactg gaggcttttg agcatcccaa tgttgtccgg ctgatggacg 421 tctgtgccac atcccgaact gaccgggaga tcaaggtaac cctggtgttt gagcatgtag 481 accaggacct aaggacatat ctggacaagg cacccccacc aggcttgcca gccgaaacga 541 tcaaggatct gatgcgccag tttctaagag gcctagattt ccttcatgcc aattgcatcg 601 ttcaccgaga tctgaagcca gagaacattc tggtgacaag tggtggaaca gtcaagctgg 661 ctgactttgg cctggccaga atctacagct accagatggc acttacaccc gtggttgtta 721 cactctggta ccgagctccc gaagttcttc tgcagtccac atatgcaaca cctgtggaca 781 tgtggagtgt tggctgtatc tttgcagaga tgtttcgtcg aaagcctctc ttctgtggaa 841 actctgaagc cgaccagttg ggcaaaatct ttgacctgat tgggctgcct ccagaggatg 901 actggcctcg agatgtatcc ctgccccgtg gagcctttcc ccccagaggg ccccgcccag 961 tgcagtcggt ggtacctgag atggaggagt cgggagcaca gctgctgctg gaaatgctga 1021 cttttaaccc acacaagcga atctctgcct ttcgagctct gcagcactct tatctacata 1081 aggatgaagg taatccggag tgattgccaa ctttcttgta caaagttggc attataagaa 1141 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta 1201 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct 1261 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat 1321 catcatgcct cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg 1381 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct 1441 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct 1501 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt 1561 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc 1621 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa 1681 agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag 1741 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg 1801 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa 1861 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc 1921 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca 1981 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca 2041 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa 2101 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg 2161 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc 2221 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg 2281 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc 2341 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag 2401 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga 2461 tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga 2521 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 2581 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 2641 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 2701 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga 2761 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 2821 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 2881 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 2941 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 3001 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 3061 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 3121 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 3181 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 3241 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca 3301 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca 3361 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg 3421 actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt 3481 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc 3541 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc 3601 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg 3661 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcct //