LOCUS       Exported                 881 bp ds-DNA     linear   SYN 03-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    7TFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Fuerer C, Nusse R
  TITLE     Lentiviral vectors to probe and manipulate the wnt signaling 
            pathway.
  JOURNAL   PLoS One. 2010  . 5(2):e9370.
  PUBMED    20186325
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 3, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    95..212
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        434..465
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      521..530
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     complement(593..611)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
       61 gattagtgaa cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg
      121 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
      181 tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc ctcctagccc
      241 gggctcgagc agctgaagct tgcatgcctg cagggtaccg agctcttacg cgagatcaaa
      301 gggggtaaga tcaaaggggg taagatcaaa ggggcgcgag atcaaagggg gtaagatcaa
      361 agggggtaag atcaaagggg gtaagatcaa aggggcgcgc ccgcgtgcta gcccgggctc
      421 gagatctaga ctctagaggg tatataatgg aagctcgaat tccagcttgg cattccggta
      481 ctgttggtaa aaagcttggc attccggtac tgttggtaaa gccaccatgg aagacgccaa
      541 aaacataaag aaaggcccgg cgccattcta tccgctggaa gatggaaccg ctggagagca
      601 actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc
      661 acatatcgag gtggacatca cttacgctga gtacttcgaa atgtccgttc ggttggcaga
      721 agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc
      781 tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc
      841 gaacgacatt tataatgaac gtgaattgct caacagtatg g
//
