LOCUS       Exported                4665 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Gateway Donor vector containing ARNTL , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    pDONR223_ARNTL_WT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             211..1002
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1095..1683
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1584..1603
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1837..1854
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      2013..2040
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      2132..2218
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2267..2289
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2281..2298
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2282..2298
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2314..2413
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4289..4388)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4405..4424)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4406..4424)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4429..4445)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4429..4445)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
       61 atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa
      121 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
      181 ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc cgaagtatcg
      241 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
      301 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg
      361 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
      421 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt
      481 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
      541 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat
      601 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
      661 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
      721 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
      781 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
      841 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct
      901 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
      961 cactacgtga aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga
     1021 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     1081 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1141 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1201 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     1261 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     1321 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     1381 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     1441 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     1501 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     1561 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     1621 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     1681 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     1741 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     1801 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     1861 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     1921 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt
     1981 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
     2041 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
     2101 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
     2161 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat
     2221 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
     2281 tgtaaaacga cggccagtct taagctcggg ccccaaataa tgattttatt ttgactgata
     2341 gtgacctgtt cgttgcaaca aattgatgag caatgctttt ttataatgcc aactttgtac
     2401 aaaaaagttg gcatggcaga ccagagaatg gacatttctt caaccatcag tgatttcatg
     2461 tccccgggcc ccaccgacct gctttccagc tctcttggta ccagtggtgt ggattgcaac
     2521 cgcaaacgga aaggcagctc cactgactac caagaaagca tggacacaga caaagatgac
     2581 cctcatggaa ggttagaata tacagaacac caaggaagga taaaaaatgc aagggaagct
     2641 cacagtcaga ttgaaaagcg gcgtcgggat aaaatgaaca gttttataga tgaattggct
     2701 tctttggtac caacatgcaa cgcaatgtcc aggaaattag ataaacttac tgtgctaagg
     2761 atggctgttc agcacatgaa aacattaaga ggtgccacca atccatacac agaagcaaac
     2821 tacaaaccaa cttttctatc agacgatgaa ttgaaacacc tcattctcag ggcagcagat
     2881 ggatttttgt ttgtcgtagg atgtgaccga gggaagatac tctttgtctc agagtctgtc
     2941 ttcaagatcc tcaactacag ccagaatgat ctgattggtc agagtttgtt tgactacctg
     3001 catcctaaag atattgccaa agtcaaggag cagctctcct cctctgacac cgcaccccgg
     3061 gagcggctca tagatgcaaa aactggactt ccagttaaaa cagatataac ccctgggcca
     3121 tctcgattat gttctggagc acgacgttct ttcttctgta ggatgaagtg taacaggcct
     3181 tcagtaaagg ttgaagacaa ggacttcccc tctacctgct caaagaaaaa agatcgaaaa
     3241 agcttctgca caatccacag cacaggctat ttgaaaagct ggccacccac aaagatgggg
     3301 ctggatgaag acaacgaacc agacaatgag gggtgtaacc tcagctgcct cgtcgcaatt
     3361 ggacgactgc attctcatgt agttccacaa ccagtgaacg gggaaatcag ggtgaaatct
     3421 atggaatatg tttctcggca cgcgatagat ggaaagtttg tttttgtaga ccagagggca
     3481 acagctattt tggcatattt accacaagaa cttctaggca catcgtgtta tgaatatttt
     3541 caccaagatg acataggaca tcttgcagaa tgtcataggc aagttttaca gacgagagaa
     3601 aaaattacaa ctaattgcta taaatttaaa atcaaagatg gttcttttat cacactacgg
     3661 agtcgatggt tcagtttcat gaacccttgg accaaggaag tagaatatat tgtctcaact
     3721 aacactgttg ttttagccaa cgtcctggaa ggcggggacc caaccttccc acagctcaca
     3781 gcatcccccc acagcatgga cagcatgctg ccctctggag aaggtggccc aaagaggacc
     3841 caccccactg ttccagggat tccaggggga acccgggctg gggcaggaaa aataggccga
     3901 atgattgctg aggaaatcat ggaaatccac aggataagag ggtcatcgcc ttctagctgt
     3961 ggctccagcc cattgaacat cacgagtacg cctccccctg atgcctcttc tccaggaggc
     4021 aagaagattt taaatggagg gactccagac attccttcca gtggcctact atcaggccag
     4081 gctcaggaga acccaggtta tccatattct gatagttctt ctattcttgg tgagaacccc
     4141 cacataggta tagacatgat tgacaacgac caaggatcaa gtagtcccag taatgatgag
     4201 gcagcaatgg ctgtcatcat gagcctcttg gaagcagatg ctggactggg tggccctgtt
     4261 gactttagtg acttgccatg gccgctgtac ccaactttct tgtacaaagt tggcattata
     4321 agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
     4381 attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt
     4441 tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     4501 atatcatcat gcctcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     4561 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     4621 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctca
//
