LOCUS       Exported                4976 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Localization: C1 Cloning Vector, Excitation: 684, Emission: 708.
ACCESSION   .
VERSION     .
KEYWORDS    IFP2.0-C1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Yu D, Gustafson WC, Han C, Lafaye C, Noirclerc-Savoye M, Ge WP, 
            Thayer DA, Huang H, Kornberg TB, Royant A, Jan LY, Jan YN, Weiss WA,
            Shu X
  TITLE     An improved monomeric infrared fluorescent protein for neuronal and 
            tumour brain imaging.
  JOURNAL   Nat Commun. 2014 May 15;5:3626. doi: 10.1038/ncomms4626.
  PUBMED    24832154
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        68..371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        372..575
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     526..546
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      614..623
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             620..1582
                     /codon_start=1
                     /product="bacteriophytochrome-based monomeric infrared 
                     fluorescent protein (Yu et al., 2014)"
                     /label=IFP2.0
                     /translation="MARDPQPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTAD
                     GHSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPDQWPALQTALPPGCQDALQYRATLD
                     WPAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQT
                     VRELSGFDRVMLYKFAPDATGEVIAEARREGMQAYLGHRFPASTTPAQARALYTRHLLR
                     LTADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGG
                     QLWGLIVCHHQTPYVLPPDLRTTLEYLGRLLSLQVQRKEA"
     misc_feature    1583..1648
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    1772..1893
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1809..1828)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1863..1882
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(1900..2355)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2037..2058)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2249..2268
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2382..2486
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2488..2845
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(2511..2531)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2696..2831
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2758..2777
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2880..3674
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2934..2953)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3544..3563
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3862..3881)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3906..3953
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4282..4870
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4771..4790
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
       61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      601 agcgctaccg gtcgccacca tggctcggga ccctcaacct ttcttccctc ctctgtatct
      661 cggaggacca gagatcacta ctgagaactg cgagcgggaa cctatacata taccaggttc
      721 tattcaacct catggagcac tgctcacagc cgatggacat tctggcgagg tgctgcaagt
      781 ttcactcaac gcagccactt tcctgggcca ggaaccaact gttctgcggg gacagaccct
      841 ggcagccctg ctgccagacc agtggcctgc tctgcagact gccctgcctc caggctgcca
      901 ggacgccctg cagtatcgcg ccaccctgga ttggcccgcc gccggacacc tgagcctgac
      961 cgtgcatagg gtggccgagc tgctgattct ggaattcgag cctactgagg catgggactc
     1021 tattggaccc cacgctctga gaaatgcaat gttcgctctg gagagtgctc ctaatctccg
     1081 ggcactggct gaagtcgcaa cccaaacagt ccgggaactg tcaggtttcg accgggtgat
     1141 gctgtacaag tttgcaccag acgcaacagg agaggttatt gccgaagcaa ggcgcgaggg
     1201 catgcaggct tacctcgggc ataggtttcc cgcatccacc acccctgcac aagctagggc
     1261 cctctacaca agacacctgc tccggctgac cgcagacacc agggctgcag cagtgcccct
     1321 cgaccccgtg ctgaatcccc agacaaatgc tcctacacct ctgggcggag ctgtcctcag
     1381 agctacatcc ccaatgcaca tgcagtacct gaggaatatg ggagtgggct cctccctgag
     1441 cgtcagcgtc gtggtcggcg gccagctgtg gggactgatt gtctgccacc atcagacacc
     1501 ctacgtgctg ccacccgatc tgcggaccac cctggagtat ctggggaggc tcctgtccct
     1561 gcaggtgcag aggaaagaag cctccggact cagatctcga gctcaagctt cgaattctgc
     1621 agtcgacggt accgcgggcc cgggatccac cggatctaga taactgatca taatcagcca
     1681 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     1741 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     1801 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     1861 ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta aattgtaagc gttaatattt
     1921 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     1981 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     2041 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     2101 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     2161 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     2221 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     2281 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     2341 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     2401 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2461 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
     2521 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     2581 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     2641 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     2701 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     2761 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     2821 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca
     2881 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     2941 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     3001 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     3061 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     3121 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     3181 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     3241 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     3301 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     3361 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     3421 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     3481 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     3541 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     3601 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     3661 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     3721 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     3781 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     3841 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     3901 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
     3961 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     4021 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     4081 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
     4141 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     4201 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4261 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4321 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4381 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     4441 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4501 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4561 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4621 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     4681 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     4741 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4801 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4861 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4921 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccg
//
