LOCUS       Exported                1059 bp ds-DNA     linear   SYN 02-JUN-2021
DEFINITION  Expresses human RagA from a CMV promoter for stable expression in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pLJM1 RagA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Efeyan A, Schweitzer LD, Bilate AM, Chang S, Kirak O, Lamming DW, 
            Sabatini DM
  TITLE     RagA, but not RagB, is essential for embryonic development and adult
            mice.
  JOURNAL   Dev Cell. 2014 May 12;29(3):321-9. doi: 
            10.1016/j.devcel.2014.03.017. Epub 2014 Apr 24.
  PUBMED    24768164
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 2, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      47..56
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             56..79
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtgcca ccatggacta
       61 caaggatgac gatgacaagg gtggtggtgg tggtgcgtcg acgatgccaa atacagccat
      121 gaagaaaaag gtgctgctga tggggaagag cgggtcgggg aagaccagca tgaggtcgat
      181 aatcttcgcc aattacattg ctcgcgacac ccggcgcctg ggggccacca ttgacgtgga
      241 acactcccac gtccgattcc tagggaacct ggtgctgaac ctgtgggact gtggcggtca
      301 ggacaccttc atggaaaatt acttcaccag ccagcgagac aatatcttcc gtaacgtgga
      361 agttttgatt tacgtgtttg acgtggagag ccgcgaactg gaaaaggaca tgcattatta
      421 ccagtcgtgt ctggaggcca tcctccagaa ctctcctgac gccaaaatct tctgcctggt
      481 gcacaaaatg gatctggttc aggaggatca gcgtgacctg atttttaaag agcgagagga
      541 agacctgagg cgtctgtctc gcccgctgga gtgtgcttgt tttcgaacgt ccatctggga
      601 tgagacgctc tacaaagcct ggtccagcat cgtctaccag ctgattccca acgttcagca
      661 gctggagatg aacctcagga attttgccca aatcattgag gccgatgaag ttctgctgtt
      721 cgaaagagct acattcttgg ttatttccca ctaccagtgc aaagagcagc gcgacgtcca
      781 ccggtttgag aagatcagca acatcatcaa acagttcaag ctgagctgca gtaaattggc
      841 cgcttccttc cagagcatgg aagttaggaa ttccaacttc gctgctttca tcgacatctt
      901 cacctcaaat acgtacgtga tggtggtcat gtcagatccg tcgatccctt ctgcggccac
      961 tctgatcaac attcgcaatg cccggaaaca ctttgagaag ctggagagag tggatggccc
     1021 caagcacagt ctccttatgc gttgacaatt ctcgacctc
//
