LOCUS       Exported                 827 bp ds-DNA     linear   SYN 27-MAY-2021
DEFINITION  Role of Rac selective GEF domain from Tiam1 in immune cell 
            migration.
ACCESSION   .
VERSION     .
KEYWORDS    mTiam1-mcherry-sspB in pcDNA3.1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 827)
  AUTHORS   O'Neill PR, Kalyanaraman V, Gautam N
  TITLE     Subcellular optogenetic activation of Cdc42 controls local and 
            distal signaling to drive immune cell migration.
  JOURNAL   Mol Biol Cell. 2016 May 1;27(9):1442-50. doi: 
            10.1091/mbc.E15-12-0832. Epub 2016 Mar 3.
  PUBMED    26941336
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 27, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(259..277)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     527..546
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gnnctnnggn gaggatttct gagtctgtgc attcgatcct gcgagataaa cacagaagac
       61 agctcctcaa aacggaagaa ttcagtgctg gatccggtag tggaagtggt tccatggtga
      121 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
      181 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
      241 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
      301 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
      361 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
      421 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
      481 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
      541 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
      601 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
      661 agaccaccct acaaggccaa agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
      721 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
      781 cgaggggccg cccacttcca ccggcggcat ggaacgagct gtacaag
//
