LOCUS       Exported                8342 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses rOpto-mFGFR1 (light-activated receptor tyrosine kinase).
ACCESSION   .
VERSION     .
KEYWORDS    rOpto-mFGFR1_333
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Reichhart E, Ingles-Prieto A, Tichy AM, McKenzie C, Janovjak H
  TITLE     A Phytochrome Sensory Domain Permits Receptor Activation by Red 
            Light.
  JOURNAL   Angew Chem Int Ed Engl. 2016 May 17;55(21):6339-42. doi: 
            10.1002/anie.201601736. Epub 2016 Apr 21.
  PUBMED    27101018
REFERENCE   2  (bases 1 to 8342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      935..944
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             941..982
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             3833..3859
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(3936..3953)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3942..4166
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4212..4640
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4299..4318)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4509..4530
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4649..4669)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4654..4983
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4834..4969
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4896..4915
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5050..5844
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5104..5123)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5714..5733
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6018..6139
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6055..6074)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6109..6128
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6188..6204)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6188..6204)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6201..6223)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6212..6228
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6236..6266)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6281..6302
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6419..6436)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6590..7175)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6670..6689)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7346..8206)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7969..7988
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8207..8311)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga ctcgagcggc cgcggccacc atggggagta gcaagagcaa
      961 gcctaaggac cccagccagc gcctcgacat gaagagcggc accaagaaga gcgacttcca
     1021 tagccagatg gctgtgcaca agctggccaa gagcatccct ctgcgcagac aggtaacagt
     1081 gtcagctgac tccagtgcat ccatgaactc tggggttctc ctggttcggc cctcacggct
     1141 ctcctccagc gggaccccca tgctggctgg agtctccgaa tatgagctcc ctgaggatcc
     1201 ccgctgggag ctgccacgag acagactggt cttaggcaaa ccacttggcg agggctgctt
     1261 cgggcaggtg gtgttggctg aggccatcgg gctggataag gacaaaccca accgtgtgac
     1321 caaagtggcc gtgaagatgt tgaagtccga cgcaacggag aaggacctgt cggatctgat
     1381 ctcggagatg gagatgatga aaatgattgg gaagcacaag aatatcatca accttctggg
     1441 agcgtgcaca caggatggtc ctctttatgt cattgtggag tacgcctcca aaggcaatct
     1501 ccgggagtat ctacaggccc ggaggcctcc tgggctggag tactgctata accccagcca
     1561 caaccccgag gaacagctgt cttccaaaga tctggtatcc tgtgcctatc aggtggctcg
     1621 gggcatggag tatcttgcct ctaagaagtg tatacaccga gacctggctg ctaggaacgt
     1681 cctggtgacc gaggataacg taatgaagat cgcagacttt ggcttagctc gagacattca
     1741 tcatatcgac tactacaaga aaaccaccaa cggccggctg cctgtgaagt ggatggcccc
     1801 tgaggcgttg tttgaccgga tctacacaca ccagagcgat gtgtggtctt ttggagtgct
     1861 cttgtgggag atcttcactc tgggtggctc cccatacccc ggtgtgcctg tggaggaact
     1921 tttcaagctg ctgaaggagg gtcatcgaat ggacaagccc agtaactgta ccaatgagct
     1981 gtacatgatg atgcgggact gctggcatgc agtgccctct cagagaccta cgttcaagca
     2041 gttggtggaa gacctggacc gcattgtggc cttgacctcc aaccaggagt atctggacct
     2101 gtccataccg ctggaccagt actcacccag ctttcccgac acacggagct ccacctgctc
     2161 ctcaggggag gactctgtct tctctcatga gccgttacct gaggagccct gtctgcctcg
     2221 acaccccacc cagcttgcca acagtggact caaacggcgc gtcgagaccg gggcaactac
     2281 tgttcaactg tctgatcaat ctctgcgtca actggaaact ctggctatcc acaccgcgca
     2341 tctgatccag ccgcacggtc tggtagtcgt cctgcaagaa ccggacctga ccatcagcca
     2401 gatctctgcg aactgtaccg gtatcctggg ccgtagcccg gaagatctgc tgggtcgtac
     2461 tctgggcgag gtattcgatt cttttcagat tgatccgatc cagtctcgtc tgaccgcagg
     2521 tcagatttcc agcctgaacc cgtccaagct gtgggcgcgt gttatgggtg acgactttgt
     2581 tattttcgac ggcgtatttc atcgtaactc tgatggcctg ctggtttgcg agctggagcc
     2641 ggcctacact agcgacaacc tgcctttcct gggtttctac catatggcaa acgcggcact
     2701 gaaccgtctg cgtcagcaag ctaacctgcg cgacttctac gacgttatcg ttgaggaagt
     2761 gcgccgcatg acgggtttcg accgcgtcat gctgtaccgt tttgatgaaa acaaccacgg
     2821 tgacgtaatc gcggaggata agcgtgacga catggagccg tatctgggtc tgcactaccc
     2881 ggaaagcgac attcctcagc cggcacgtcg cctgttcatt cacaacccga tccgtgttat
     2941 tccggacgtt tacggcgttg ctgttccgct gactccggcc gttaatccgt ctactaaccg
     3001 tgcagttgac ctgaccgaat ccatcctgcg ttccgcatac cattgccacc tgacctatct
     3061 gaagaacatg ggcgttggtg ctagcctgac gatctctctg attaaagatg gtcacctgtg
     3121 gggtctgatc gcttgccatc accagacccc gaaagtaatc cctttcgaac tgcgtaaagc
     3181 ctgcgaattc ttcggtcgtg tggtgttctc taatatctcc gcgcaagaag acaccgagac
     3241 ttttgactac cgcgtacagc tggcggagca tgaagcggtt ctgctggaca aaatgaccac
     3301 cgcggcagac ttcgtggagg gcctgactaa ccacccagac cgtctgctgg gcctgaccgg
     3361 cagccaaggc gctgcgattt gtttcggcga gaaactgatt ctggtgggcg aaaccccaga
     3421 cgaaaaggcg gtgcaatacc tgctgcaatg gctggagaat cgcgaagtgc aggacgtttt
     3481 cttcactagc tctctgtctc agatctatcc ggatgcggtt aacttcaaaa gcgtggcgtc
     3541 cggcctgctg gctatcccga tcgcccgtca taactttctg ctgtggttcc gcccggaggt
     3601 tctgcagacc gttaattggg gtggtgatcc gaatcacgca tacgaagcaa cccaagaaga
     3661 tggtaagatc gaactgcatc cgcgtcagtc cttcgatctg tggaaagaaa ttgttcgcct
     3721 gcagagcctg ccgtggcaga gcgttgagat ccagtctgcc ctggctctga agaaagcaat
     3781 cgtgaacctg attctgcgcc aagctgaaga acccggtgga tccggagtcg actatccgta
     3841 cgacgtacca gactacgcac tcgactaaga attccaccac actggactag tggatccgag
     3901 ctcggtacca agcttaagtt taaaccgctg atcagcctcg actgtgcctt ctagttgcca
     3961 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     4021 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     4081 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaga atagcaggca
     4141 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     4201 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4261 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4321 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4381 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     4441 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4501 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     4561 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4621 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     4681 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     4741 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4801 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4861 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4921 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     4981 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     5041 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     5101 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     5161 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     5221 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     5281 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     5341 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     5401 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     5461 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     5521 ctggacgaag aacatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     5581 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     5641 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     5701 tatcaggaca tagcgttggc tacccgtgat attgctgaag aacttggcgg cgaatgggct
     5761 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     5821 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     5881 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     5941 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     6001 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6061 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6121 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     6181 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     6241 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     6301 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     6361 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tgcgcttcct
     6421 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6481 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6541 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6601 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6661 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6721 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6781 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6841 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6901 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6961 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7021 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7081 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca
     7141 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7201 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7261 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7321 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7381 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7441 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgggac ccacgctcac
     7501 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7561 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7621 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7681 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7741 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7801 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7861 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7921 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7981 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     8041 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8101 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8161 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     8221 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     8281 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     8341 tc
//
