LOCUS       Exported               12433 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses human codon-optimized LbCpf1 protein and blasticidin 
            resistance from EFS promoter. Lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    Lenti-LbCpf1-Blast
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12433)
  AUTHORS   Kim HK, Song M, Lee J, Menon AV, Jung S, Kang YM, Choi JW, Woo E, 
            Koh HC, Nam JW, Kim H
  TITLE     In vivo high-throughput profiling of CRISPR-Cpf1 activity.
  JOURNAL   Nat Methods. 2016 Dec 19. doi: 10.1038/nmeth.4104.
  PUBMED    27992409
REFERENCE   2  (bases 1 to 12433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12433
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2650..2861
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2903..6586
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             6587..6634
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6644..6700
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6701..7099
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    7124..7712
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7177..7197)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7595..7606)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7715..7731)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7716..7732)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8237..8417
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8443..8460)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8449..8673
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8719..9147
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8806..8825)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9016..9037
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9156..9176)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9161..9491
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9343..9477
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9404..9423
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9539..9586
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9605..9979
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10109..10230
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10146..10165)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10200..10219
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10279..10295)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10279..10295)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10292..10314)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10303..10319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10327..10357)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10372..10393
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10510..10527)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10681..11269)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10761..10780)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11440..12300)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12063..12082
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12301..12405)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagctagg tcttgaaagg agtgggaatt ggctccggtg
     2641 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     2701 gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     2761 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     2821 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggaccggttc tagcgtttaa
     2881 acttaagctt ggtaccgcca ccatgagcaa gctggagaag tttacaaact gctactccct
     2941 gtctaagacc ctgaggttca aggccatccc tgtgggcaag acccaggaga acatcgacaa
     3001 taagcggctg ctggtggagg acgagaagag agccgaggat tataagggcg tgaagaagct
     3061 gctggatcgc tactatctgt cttttatcaa cgacgtgctg cacagcatca agctgaagaa
     3121 tctgaacaat tacatcagcc tgttccggaa gaaaaccaga accgagaagg agaataagga
     3181 gctggagaac ctggagatca atctgcggaa ggagatcgcc aaggccttca agggcaacga
     3241 gggctacaag tccctgttta agaaggatat catcgagaca atcctgccag agttcctgga
     3301 cgataaggac gagatcgccc tggtgaacag cttcaatggc tttaccacag ccttcaccgg
     3361 cttctttgat aacagagaga atatgttttc cgaggaggcc aagagcacat ccatcgcctt
     3421 caggtgtatc aacgagaatc tgacccgcta catctctaat atggacatct tcgagaaggt
     3481 ggacgccatc tttgataagc acgaggtgca ggagatcaag gagaagatcc tgaacagcga
     3541 ctatgatgtg gaggatttct ttgagggcga gttctttaac tttgtgctga cacaggaggg
     3601 catcgacgtg tataacgcca tcatcggcgg cttcgtgacc gagagcggcg agaagatcaa
     3661 gggcctgaac gagtacatca acctgtataa tcagaaaacc aagcagaagc tgcctaagtt
     3721 taagccactg tataagcagg tgctgagcga tcgggagtct ctgagcttct acggcgaggg
     3781 ctatacatcc gatgaggagg tgctggaggt gtttagaaac accctgaaca agaacagcga
     3841 gatcttcagc tccatcaaga agctggagaa gctgttcaag aattttgacg agtactctag
     3901 cgccggcatc tttgtgaaga acggccccgc catcagcaca atctccaagg atatcttcgg
     3961 cgagtggaac gtgatccggg acaagtggaa tgccgagtat gacgatatcc acctgaagaa
     4021 gaaggccgtg gtgaccgaga agtacgagga cgatcggaga aagtccttca agaagatcgg
     4081 ctccttttct ctggagcagc tgcaggagta cgccgacgcc gatctgtctg tggtggagaa
     4141 gctgaaggag atcatcatcc agaaggtgga tgagatctac aaggtgtatg gctcctctga
     4201 gaagctgttc gacgccgatt ttgtgctgga gaagagcctg aagaagaacg acgccgtggt
     4261 ggccatcatg aaggacctgc tggattctgt gaagagcttc gagaattaca tcaaggcctt
     4321 ctttggcgag ggcaaggaga caaacaggga cgagtccttc tatggcgatt ttgtgctggc
     4381 ctacgacatc ctgctgaagg tggaccacat ctacgatgcc atccgcaatt atgtgaccca
     4441 gaagccctac tctaaggata agttcaagct gtattttcag aaccctcagt tcatgggcgg
     4501 ctgggacaag gataaggaga cagactatcg ggccaccatc ctgagatacg gctccaagta
     4561 ctatctggcc atcatggata agaagtacgc caagtgcctg cagaagatcg acaaggacga
     4621 tgtgaacggc aattacgaga agatcaacta taagctgctg cccggcccta ataagatgct
     4681 gccaaaggtg ttcttttcta agaagtggat ggcctactat aaccccagcg aggacatcca
     4741 gaagatctac aagaatggca cattcaagaa gggcgatatg tttaacctga atgactgtca
     4801 caagctgatc gacttcttta aggatagcat ctcccggtat ccaaagtggt ccaatgccta
     4861 cgatttcaac ttttctgaga cagagaagta taaggacatc gccggctttt acagagaggt
     4921 ggaggagcag ggctataagg tgagcttcga gtctgccagc aagaaggagg tggataagct
     4981 ggtggaggag ggcaagctgt atatgttcca gatctataac aaggactttt ccgataagtc
     5041 tcacggcaca cccaatctgc acaccatgta cttcaagctg ctgtttgacg agaacaatca
     5101 cggacagatc aggctgagcg gaggagcaga gctgttcatg aggcgcgcct ccctgaagaa
     5161 ggaggagctg gtggtgcacc cagccaactc ccctatcgcc aacaagaatc cagataatcc
     5221 caagaaaacc acaaccctgt cctacgacgt gtataaggat aagaggtttt ctgaggacca
     5281 gtacgagctg cacatcccaa tcgccatcaa taagtgcccc aagaacatct tcaagatcaa
     5341 tacagaggtg cgcgtgctgc tgaagcacga cgataacccc tatgtgatcg gcatcgatag
     5401 gggcgagcgc aatctgctgt atatcgtggt ggtggacggc aagggcaaca tcgtggagca
     5461 gtattccctg aacgagatca tcaacaactt caacggcatc aggatcaaga cagattacca
     5521 ctctctgctg gacaagaagg agaaggagag gttcgaggcc cgccagaact ggacctccat
     5581 cgagaatatc aaggagctga aggccggcta tatctctcag gtggtgcaca agatctgcga
     5641 gctggtggag aagtacgatg ccgtgatcgc cctggaggac ctgaactctg gctttaagaa
     5701 tagccgcgtg aaggtggaga agcaggtgta tcagaagttc gagaagatgc tgatcgataa
     5761 gctgaactac atggtggaca agaagtctaa tccttgtgca acaggcggcg ccctgaaggg
     5821 ctatcagatc accaataagt tcgagagctt taagtccatg tctacccaga acggcttcat
     5881 cttttacatc cctgcctggc tgacatccaa gatcgatcca tctaccggct ttgtgaacct
     5941 gctgaaaacc aagtatacca gcatcgccga ttccaagaag ttcatcagct cctttgacag
     6001 gatcatgtac gtgcccgagg aggatctgtt cgagtttgcc ctggactata agaacttctc
     6061 tcgcacagac gccgattaca tcaagaagtg gaagctgtac tcctacggca accggatcag
     6121 aatcttccgg aatcctaaga agaacaacgt gttcgactgg gaggaggtgt gcctgaccag
     6181 cgcctataag gagctgttca acaagtacgg catcaattat cagcagggcg atatcagagc
     6241 cctgctgtgc gagcagtccg acaaggcctt ctactctagc tttatggccc tgatgagcct
     6301 gatgctgcag atgcggaaca gcatcacagg ccgcaccgac gtggattttc tgatcagccc
     6361 tgtgaagaac tccgacggca tcttctacga tagccggaac tatgaggccc aggagaatgc
     6421 catcctgcca aagaacgccg acgccaatgg cgcctataac atcgccagaa aggtgctgtg
     6481 ggccatcggc cagttcaaga aggccgagga cgagaagctg gataaggtga agatcgccat
     6541 ctctaacaag gagtggctgg agtacgccca gaccagcgtg aagcacaaaa ggccggcggc
     6601 cacgaaaaag gccggccagg caaaaaagaa aaagggatcc ggcgcaacaa acttctctct
     6661 gctgaaacaa gccggagatg tcgaagagaa tcctggaccg atggccaagc ctttgtctca
     6721 agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga
     6781 agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa
     6841 tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc
     6901 tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt
     6961 gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat
     7021 agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg
     7081 ttatgtgtgg gagggctaag aattcgatat caagcttatc ggtaatcaac ctctggatta
     7141 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     7201 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     7261 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     7321 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     7381 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     7441 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     7501 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     7561 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     7621 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     7681 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagacctaga
     7741 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
     7801 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc
     7861 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7921 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca
     7981 aggctacttc cctgattggc agaactacac accagggcca gggatcagat atccactgac
     8041 ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga
     8101 aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     8161 agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct
     8221 gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     8281 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     8341 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8401 gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     8461 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     8521 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     8581 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     8641 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     8701 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     8761 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     8821 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     8881 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     8941 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     9001 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     9061 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     9121 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     9181 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     9241 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     9301 caattagtca gcaaccatag tcccgcccct aactccgccc agtcccgccc ctaactccgc
     9361 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     9421 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     9481 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
     9541 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     9601 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
     9661 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
     9721 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
     9781 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
     9841 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
     9901 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
     9961 ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga
    10021 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
    10081 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
    10141 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    10201 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
    10261 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
    10321 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    10381 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    10441 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    10501 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    10561 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    10621 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    10681 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    10741 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    10801 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    10861 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    10921 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    10981 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    11041 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    11101 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    11161 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    11221 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    11281 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    11341 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    11401 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    11461 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    11521 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    11581 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    11641 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    11701 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    11761 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    11821 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    11881 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    11941 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    12001 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    12061 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    12121 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    12181 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    12241 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    12301 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    12361 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    12421 agtgccacct gac
//
