LOCUS Exported 7070 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses CPH1S-o domain (Synechocystis Phytochrome 1) preceded by a MYR signal and a restriction site. ACCESSION . VERSION . KEYWORDS MYR-CPH1S-o_445 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7070) AUTHORS Reichhart E, Ingles-Prieto A, Tichy AM, McKenzie C, Janovjak H TITLE A Phytochrome Sensory Domain Permits Receptor Activation by Red Light. JOURNAL Angew Chem Int Ed Engl. 2016 May 17;55(21):6339-42. doi: 10.1002/anie.201601736. Epub 2016 Apr 21. PUBMED 27101018 REFERENCE 2 (bases 1 to 7070) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7070 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 935..944 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 941..982 /codon_start=1 /product="N-myristoylation signal from Src kinase (Pellman et al., 1985; Kaplan et al., 1988)" /label=myr /translation="MGSSKSKPKDPSQR" CDS 2561..2587 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" primer_bind complement(2664..2681) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 2670..2894 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 2940..3368 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3027..3046) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3237..3258 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(3377..3397) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 3382..3711 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 3562..3697 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3624..3643 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3778..4572 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3832..3851) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4442..4461 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 4746..4867 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4783..4802) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4837..4856 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(4916..4932) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4916..4932) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4929..4951) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4940..4956 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4964..4994) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5009..5030 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(5147..5164) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5318..5903) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5398..5417) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6074..6934) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6697..6716 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6935..7039) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaacgg gccctctaga ctcgagcggc cgcggccacc atggggagta gcaagagcaa 961 gcctaaggac cccagccagc gcctcgacgt caccggtgga gcaactactg ttcaactgtc 1021 tgatcaatct ctgcgtcaac tggaaactct ggctatccac accgcgcatc tgatccagcc 1081 gcacggtctg gtagtcgtcc tgcaagaacc ggacctgacc atcagccaga tctctgcgaa 1141 ctgtaccggt atcctgggcc gtagcccgga agatctgctg ggtcgtactc tgggcgaggt 1201 attcgattct tttcagattg atccgatcca gtctcgtctg accgcaggtc agatttccag 1261 cctgaacccg tccaagctgt gggcgcgtgt tatgggtgac gactttgtta ttttcgacgg 1321 cgtatttcat cgtaactctg atggcctgct ggtttgcgag ctggagccgg cctacactag 1381 cgacaacctg cctttcctgg gtttctacca tatggcaaac gcggcactga accgtctgcg 1441 tcagcaagct aacctgcgcg acttctacga cgttatcgtt gaggaagtgc gccgcatgac 1501 gggtttcgac cgcgtcatgc tgtaccgttt tgatgaaaac aaccacggtg acgtaatcgc 1561 ggaggataag cgtgacgaca tggagccgta tctgggtctg cactacccgg aaagcgacat 1621 tcctcagccg gcacgtcgcc tgttcattca caacccgatc cgtgttattc cggacgttta 1681 cggcgttgct gttccgctga ctccggccgt taatccgtct actaaccgtg cagttgacct 1741 gaccgaatcc atcctgcgtt ccgcatacca ttgccacctg acctatctga agaacatggg 1801 cgttggtgct agcctgacga tctctctgat taaagatggt cacctgtggg gtctgatcgc 1861 ttgccatcac cagaccccga aagtaatccc tttcgaactg cgtaaagcct gcgaattctt 1921 cggtcgtgtg gtgttctcta atatctccgc gcaagaagac accgagactt ttgactaccg 1981 cgtacagctg gcggagcatg aagcggttct gctggacaaa atgaccaccg cggcagactt 2041 cgtggagggc ctgactaacc acccagaccg tctgctgggc ctgaccggca gccaaggcgc 2101 tgcgatttgt ttcggcgaga aactgattct ggtgggcgaa accccagacg aaaaggcggt 2161 gcaatacctg ctgcaatggc tggagaatcg cgaagtgcag gacgttttct tcactagctc 2221 tctgtctcag atctatccgg atgcggttaa cttcaaaagc gtggcgtccg gcctgctggc 2281 tatcccgatc gcccgtcata actttctgct gtggttccgc ccggaggttc tgcagaccgt 2341 taattggggt ggtgatccga atcacgcata cgaagcaacc caagaagatg gtaagatcga 2401 actgcatccg cgtcagtcct tcgatctgtg gaaagaaatt gttcgcctgc agagcctgcc 2461 gtggcagagc gttgagatcc agtctgccct ggctctgaag aaagcaatcg tgaacctgat 2521 tctgcgccaa gctgaagaac ccggtggatc cggagtcgac tatccgtacg acgtaccaga 2581 ctacgcactc gactaagaat tccaccacac tggactagtg gatccgagct cggtaccaag 2641 cttaagttta aaccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt 2701 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 2761 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 2821 ggtggggcag gacagcaagg gggaggattg ggaagagaat agcaggcatg ctggggatgc 2881 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca 2941 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 3001 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 3061 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag 3121 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 3181 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 3241 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 3301 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 3361 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 3421 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 3481 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 3541 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 3601 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag 3661 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg 3721 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg 3781 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc 3841 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg 3901 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag 3961 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc 4021 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat 4081 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg 4141 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc 4201 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagaa 4261 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc 4321 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc 4381 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata 4441 gcgttggcta cccgtgatat tgctgaagaa cttggcggcg aatgggctga ccgcttcctc 4501 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac 4561 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc 4621 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt 4681 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc 4741 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 4801 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 4861 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat 4921 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 4981 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 5041 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 5101 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttg cgcttcctcg ctcactgact 5161 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 5221 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 5281 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 5341 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 5401 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 5461 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 5521 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 5581 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 5641 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 5701 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 5761 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 5821 cttgatccgg caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta 5881 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 5941 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 6001 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 6061 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 6121 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 6181 taccatctgg ccccagtgct gcaatgatac cgcgggaccc acgctcaccg gctccagatt 6241 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 6301 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 6361 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 6421 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 6481 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 6541 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 6601 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 6661 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 6721 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 6781 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 6841 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 6901 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 6961 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 7021 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc //