LOCUS Exported 3351 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Gateway Donor vector containing CBX1 , part of the Target Accelerator Plasmid Collection.. ACCESSION . VERSION . KEYWORDS pDONR223_CBX1_WT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3351) AUTHORS Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn WC TITLE Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. JOURNAL Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4. PUBMED 27147599 REFERENCE 2 (bases 1 to 3351) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3351 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1230..1329) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1346..1365) /label=T7 /note="T7 promoter, forward primer" promoter complement(1347..1365) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1370..1386) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1370..1386) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 1817..2608 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 2701..3289 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3190..3209 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agttggcacc atggggaaaa aacaaaacaa gaagaaagtg gaggaggtgc tagaagagga 721 ggaagaggaa tatgtggtgg aaaaagttct cgaccgtcga gtggtaaagg gcaaagtgga 781 gtacctccta aagtggaagg gattctcaga tgaggacaac acatgggagc cagaagagaa 841 cctggattgc cccgacctca ttgctgagtt tctgcagtca cagaaaacag cacatgagac 901 agataaatca gagggaggca agcgcaaagc tgattctgat tctgaagata agggagagga 961 gagcaaacca aagaagaaga aagaagagtc agaaaagcca cgaggctttg ctcgaggttt 1021 ggagccggag cggattattg gagctacaga ctccagtgga gagctcatgt tcctgatgaa 1081 atggaaaaac tctgatgagg ctgacctggt ccctgccaag gaagccaatg tcaagtgccc 1141 acaggttgtc atatccttct atgaggaaag gctgacgtgg cattcctacc cctcggagga 1201 tgatgacaaa aaagatgaca agaactaatt gccaactttc ttgtacaaag ttggcattat 1261 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat 1321 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt 1381 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa 1441 tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt cgctgctacc 1501 caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata 1561 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag 1621 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga 1681 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt 1741 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa 1801 aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga 1861 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg 1921 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac 1981 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc 2041 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga 2101 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa 2161 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct 2221 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga 2281 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc 2341 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta 2401 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg 2461 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga 2521 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg 2581 cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa aatcccttaa 2641 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 2701 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 2761 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 2821 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 2881 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 2941 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 3001 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 3061 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 3121 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 3181 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 3241 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 3301 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt t //