LOCUS Exported 4758 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway Donor vector containing HSPA5 , part of the Target Accelerator Plasmid Collection.. ACCESSION . VERSION . KEYWORDS pDONR223_HSPA5_WT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4758) AUTHORS Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn WC TITLE Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. JOURNAL Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4. PUBMED 27147599 REFERENCE 2 (bases 1 to 4758) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4758 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2637..2736) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2753..2772) /label=T7 /note="T7 promoter, forward primer" promoter complement(2754..2772) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2777..2793) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2777..2793) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 3224..4015 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 4108..4696 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4597..4616 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agttggcacc atgaagctct ccctggtggc cgcgatgctg ctgctgctca gcgcggcgcg 721 ggccgaggag gaggacaaga aggaggacgt gggcacggtg gtcggcatcg acctggggac 781 cacctactcc tgcgtcggcg tgttcaagaa cggccgcgtg gagatcatcg ccaacgatca 841 gggcaaccgc atcacgccgt cctatgtcgc cttcactcct gaaggggaac gtctgattgg 901 cgatgccgcc aagaaccagc tcacctccaa ccccgagaac acggtctttg acgccaagcg 961 gctcatcggc cgcacgtgga atgacccgtc tgtgcagcag gacatcaagt tcttgccgtt 1021 caaggtggtt gaaaagaaaa ctaaaccata cattcaagtt gatattggag gtgggcaaac 1081 aaagacattt gctcctgaag aaatttctgc catggttctc actaaaatga aagaaaccgc 1141 tgaggcttat ttgggaaaga aggttaccca tgcagttgtt actgtaccag cctattttaa 1201 tgatgcccaa cgccaagcaa ccaaagacgc tggaactatt gctggcctaa atgttatgag 1261 gatcatcaac gagcctacgg cagctgctat tgcttatggc ctggataaga gggaggggga 1321 gaagaacatc ctggtgtttg acctgggtgg cggaaccttc gatgtgtctc ttctcaccat 1381 tgacaatggt gtcttcgaag ttgtggccac taatggagat actcatctgg gtggagaaga 1441 ctttgaccag cgtgtcatgg aacacttcat caaactgtac aaaaagaaga cgggcaaaga 1501 tgtcaggaaa gacaatagag ctgtgcagaa actccggcgc gaggtagaaa aggccaaacg 1561 ggccctgtct tctcagcatc aagcaagaat tgaaattgag tccttctatg aaggagaaga 1621 cttttctgag accctgactc gggccaaatt tgaagagctc aacatggatc tgttccggtc 1681 tactatgaag cccgtccaga aagtgttgga agattctgat ttgaagaagt ctgatattga 1741 tgaaattgtt cttgttggtg gctcgactcg aattccaaag attcagcaac tggttaaaga 1801 gttcttcaat ggcaaggaac catcccgtgg cataaaccca gatgaagctg tagcgtatgg 1861 tgctgctgtc caggctggtg tgctctctgg tgatcaagat acaggtgacc tggtactgct 1921 tgatgtatgt ccccttacac ttggtattga aactgtggga ggtgtcatga ccaaactgat 1981 tccaaggaac acagtggtgc ctaccaagaa gtctcagatc ttttctacag cttctgataa 2041 tcaaccaact gttacaatca aggtctatga aggtgaaaga cccctgacaa aagacaatca 2101 tcttctgggt acatttgatc tgactggaat tcctcctgct cctcgtgggg tcccacagat 2161 tgaagtcacc tttgagatag atgtgaatgg tattcttcga gtgacagctg aagacaaggg 2221 tacagggaac aaaaataaga tcacaatcac caatgaccag aatcgcctga cacctgaaga 2281 aatcgaaagg atggttaatg atgctgagaa gtttgctgag gaagacaaaa agctcaagga 2341 gcgcattgat actagaaatg agttggaaag ctatgcctat tctctaaaga atcagattgg 2401 agataaagaa aagctgggag gtaaactttc ctctgaagat aaggagacca tggaaaaagc 2461 tgtagaagaa aagattgaat ggctggaaag ccaccaagat gctgacattg aagacttcaa 2521 agctaagaag aaggaactgg aagaaattgt tcaaccaatt atcagcaaac tctatggaag 2581 tgcaggccct cccccaactg gtgaagagga tacagcagaa aaagatgagt tgtagttgcc 2641 aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga 2701 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag 2761 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct 2821 ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga ccagccagga 2881 cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg 2941 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta 3001 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc 3061 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc 3121 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga 3181 tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat 3241 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc 3301 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag 3361 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt 3421 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt 3481 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga 3541 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac 3601 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt 3661 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct 3721 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt 3781 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga 3841 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga 3901 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt 3961 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc 4021 gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga 4081 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 4141 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 4201 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct 4261 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 4321 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 4381 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 4441 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 4501 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 4561 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 4621 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 4681 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 4741 gccttttgct cacatgtt //