LOCUS       Exported                 618 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  Lentiviral Sp dCas9-p300-P2A-PuroR.
ACCESSION   .
VERSION     .
KEYWORDS    pLV-dCas9-p300-P2A-PuroR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, 
            Crawford GE, Reddy TE, Gersbach CA
  TITLE     CRISPR-Cas9 epigenome editing enables high-throughput screening for 
            functional regulatory elements in the human genome.
  JOURNAL   Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853.
  PUBMED    28369033
REFERENCE   2  (bases 1 to 618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(122..142)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 attttgtaat ccagaggttg attgtcgact taacgcgttc aggcaccggg cttgcgggtc
       61 atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc
      121 cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg
      181 cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg
      241 tcgggcgaga ctccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc
      301 aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc
      361 gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc
      421 accgcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg
      481 aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac ggtgtggcgc
      541 gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg gacgtcgtcg
      601 cgggtggcga ggcgcacc
//
