LOCUS Exported 4236 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway Donor vector containing FYN , part of the Target Accelerator Plasmid Collection.. ACCESSION . VERSION . KEYWORDS pDONR223_FYN_WT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4236) AUTHORS Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn WC TITLE Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. JOURNAL Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4. PUBMED 27147599 REFERENCE 2 (bases 1 to 4236) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4236 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 43..65 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 57..74 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 58..74 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 90..189 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1636..1735) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1752..1771) /label=T7 /note="T7 promoter, forward primer" promoter complement(1753..1771) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1776..1792) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1776..1792) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 2223..3014 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 3107..3695 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3596..3615 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3849..3866 /label=L4440 /note="L4440 vector, forward primer" terminator 4025..4052 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 4144..4230 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta 61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga 121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa 181 aagttggcat gggctgtgtg caatgtaagg ataaagaagc aacaaaactg acggaggaga 241 gggacggcag cctgaaccag agctctgggt accgctatgg cacagacccc acccctcagc 301 actaccccag cttcggtgtg acctccatcc ccaactacaa caacttccac gcagccgggg 361 gccaaggact caccgtcttt ggaggtgtga actcttcgtc tcatacgggg accttgcgta 421 cgagaggagg aacaggagtg acactctttg tggcccttta tgactatgaa gcacggacag 481 aagatgacct gagttttcac aaaggagaaa aatttcaaat attgaacagc tcggaaggag 541 attggtggga agcccgctcc ttgacaactg gagagacagg ttacattccc agcaattatg 601 tggctccagt tgactctatc caggcagaag agtggtactt tggaaaactt ggccgaaaag 661 atgctgagcg acagctattg tcctttggaa acccaagagg tacctttctt atccgcgaga 721 gtgaaaccac caaaggtgcc tattcacttt ctatccgtga ttgggatgat atgaaaggag 781 accatgtcaa acattataaa attcgcaaac ttgacaatgg tggatactac attaccaccc 841 gggcccagtt tgaaacactt cagcagcttg tacaacatta ctcaggtacc tggaatggaa 901 acacaaaagt agccataaag actcttaaac caggcacaat gtcccccgaa tcattccttg 961 aggaagcgca gatcatgaag aagctgaagc acgacaagct ggtccagctc tatgcagtgg 1021 tgtctgagga gcccatctac atcgtcaccg agtatatgaa caaaggaagt ttactggatt 1081 tcttaaaaga tggagaagga agagctctga aattaccaaa tcttgtggac atggcagcac 1141 aggtggctgc aggaatggct tacatcgagc gcatgaatta tatccataga gatctgcgat 1201 cagcaaacat tctagtgggg aatggactca tatgcaagat tgctgacttc ggattggccc 1261 gattgataga agacaatgag tacacagcaa gacaaggtgc aaagttcccc atcaagtgga 1321 cggcccccga ggcagccctg tacgggaggt tcacaatcaa gtctgacgtg tggtcttttg 1381 gaatcttact cacagagctg gtcaccaaag gaagagtgcc atacccaggc atgaacaacc 1441 gggaggtgct ggagcaggtg gagcgaggct acaggatgcc ctgcccgcag gactgcccca 1501 tctctctgca tgagctcatg atccactgct ggaaaaagga ccctgaagaa cgccccactt 1561 ttgagtactt gcagagcttc ctggaagact actttaccgc gacagagccc cagtaccaac 1621 ctggtgaaaa cctgtaccca actttcttgt acaaagttgg cattataaga aagcattgct 1681 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc 1741 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct 1801 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc 1861 tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt 1921 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc 1981 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac 2041 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg 2101 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga 2161 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca 2221 ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca 2281 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg 2341 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg 2401 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga 2461 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc 2521 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag 2581 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag 2641 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac 2701 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg 2761 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg 2821 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt 2881 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg 2941 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg 3001 tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt 3061 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 3121 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 3181 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 3241 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 3301 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 3361 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 3421 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 3481 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 3541 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 3601 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 3661 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 3721 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 3781 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 3841 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc 3901 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 3961 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa 4021 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg 4081 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg 4141 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca 4201 gtcttccgac tgagcctttc gttttatttg atgcct //