LOCUS Exported 4221 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway Donor vector containing PPARG , part of the Target Accelerator Plasmid Collection.. ACCESSION . VERSION . KEYWORDS pDONR223_PPARG_WT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4221) AUTHORS Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn WC TITLE Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. JOURNAL Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4. PUBMED 27147599 REFERENCE 2 (bases 1 to 4221) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4221 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 43..65 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 57..74 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 58..74 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 90..189 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1621..1720) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1737..1756) /label=T7 /note="T7 promoter, forward primer" promoter complement(1738..1756) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1761..1777) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1761..1777) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 2208..2999 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 3092..3680 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3581..3600 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3834..3851 /label=L4440 /note="L4440 vector, forward primer" terminator 4010..4037 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 4129..4215 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta 61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga 121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa 181 aagttggcat gaccatggtt gacacagaga tgccattctg gcccaccaac tttgggatca 241 gctccgtgga tctctccgta atggaagacc actcccactc ctttgatatc aagcccttca 301 ctactgttga cttctccagc atttctactc cacattacga agacattcca ttcacaagaa 361 cagatccagt ggttgcagat tacaagtatg acctgaaact tcaagagtac caaagtgcaa 421 tcaaagtgga gcctgcatct ccaccttatt attctgagaa gactcagctc tacaataagc 481 ctcatgaaga gccttccaac tccctcatgg caattgaatg tcgtgtctgt ggagataaag 541 cttctggatt tcactatgga gttcatgctt gtgaaggatg caagggtttc ttccggagaa 601 caatcagatt gaagcttatc tatgacagat gtgatcttaa ctgtcggatc cacaaaaaaa 661 gtagaaataa atgtcagtac tgtcggtttc agaaatgcct tgcagtgggg atgtctcata 721 atgccatcag gtttgggcgg atgccacagg ccgagaagga gaagctgttg gcggagatct 781 ccagtgatat cgaccagctg aatccagagt ccgctgacct ccgggccctg gcaaaacatt 841 tgtatgactc atacataaag tccttcccgc tgaccaaagc aaaggcgagg gcgatcttga 901 caggaaagac aacagacaaa tcaccattcg ttatctatga catgaattcc ttaatgatgg 961 gagaagataa aatcaagttc aaacacatca cccccctgca ggagcagagc aaagaggtgg 1021 ccatccgcat ctttcagggc tgccagtttc gctccgtgga ggctgtgcag gagatcacag 1081 agtatgccaa aagcattcct ggttttgtaa atcttgactt gaacgaccaa gtaactctcc 1141 tcaaatatgg agtccacgag atcatttaca caatgctggc ctccttgatg aataaagatg 1201 gggttctcat atccgagggc caaggcttca tgacaaggga gtttctaaag agcctgcgaa 1261 agccttttgg tgactttatg gagcccaagt ttgagtttgc tgtgaagttc aatgcactgg 1321 aattagatga cagcgacttg gcaatattta ttgctgtcat tattctcagt ggagaccgcc 1381 caggtttgct gaatgtgaag cccattgaag acattcaaga caacctgcta caagccctgg 1441 agctccagct gaagctgaac caccctgagt cctcacagct gtttgccaag ctgctccaga 1501 aaatgacaga cctcagacag attgtcacgg aacacgtgca gctactgcag gtgatcaaga 1561 agacggagac agacatgagt cttcacccgc tcctgcagga gatctacaag gacttgtact 1621 acccaacttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca 1681 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct 1741 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa 1801 atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc 1861 aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga 1921 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca 1981 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg 2041 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc 2101 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca 2161 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg 2221 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg 2281 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac 2341 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag 2401 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg 2461 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc 2521 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag 2581 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct 2641 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg 2701 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa 2761 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga 2821 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac 2881 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc 2941 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac 3001 cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt 3061 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 3121 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 3181 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 3241 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 3301 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 3361 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 3421 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 3481 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 3541 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 3601 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 3661 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 3721 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 3781 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 3841 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 3901 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 3961 acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc 4021 cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg 4081 ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact 4141 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc 4201 ctttcgtttt atttgatgcc t //