LOCUS       Exported                4566 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Gateway Donor vector containing PRKCZ , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    pDONR223_PRKCZ_WT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1966..2065)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2082..2101)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2083..2101)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2106..2122)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2106..2122)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2553..3344
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3437..4025
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3926..3945
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4179..4196
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      4355..4382
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      4474..4560
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat gcccagcagg accggcccca agatggaagg gagcggcggc cgcgtccgcc
      241 tcaaggcgca ttacgggggg gacatcttca tcaccagcgt ggacgccgcc acgaccttcg
      301 aggagctctg tgaggaagtg agagacatgt gtcgtctgca ccagcagcac ccgctcaccc
      361 tcaagtgggt ggacagcgaa ggtgaccctt gcacggtgtc ctcccagatg gagctggaag
      421 aggctttccg cctggcccgt cagtgcaggg atgaaggcct catcattcat gttttcccga
      481 gcacccctga gcagcctggc ctgccatgtc cgggagaaga caaatctatc taccgccggg
      541 gagccagaag atggaggaag ctgtaccgtg ccaacggcca cctcttccaa gccaagcgct
      601 ttaacaggag agcgtactgc ggtcagtgca gcgagaggat atggggcctc gcgaggcaag
      661 gctacaggtg catcaactgc aaactgctgg tccataagcg ctgccacggc ctcgtcccgc
      721 tgacctgcag gaagcatatg gattctgtca tgccttccca agagcctcca gtagacgaca
      781 agaacgagga cgccgacctt ccttccgagg agacagatgg aattgcttac atttcctcat
      841 cccggaagca tgacagcatt aaagacgact cggaggacct taagccagtt atcgatggga
      901 tggatggaat caaaatctct caggggcttg ggctgcagga ctttgaccta atcagagtca
      961 tcgggcgcgg gagctacgcc aaggttctcc tggtgcggtt gaagaagaat gaccaaattt
     1021 acgccatgaa agtggtgaag aaagagctgg tgcatgatga cgaggatatt gactgggtac
     1081 agacagagaa gcacgtgttt gagcaggcat ccagcaaccc cttcctggtc ggattacact
     1141 cctgcttcca gacgacaagt cggttgttcc tggtcattga gtacgtcaac ggcggggacc
     1201 tgatgttcca catgcagagg cagaggaagc tccctgagga gcacgccagg ttctacgcgg
     1261 ccgagatctg catcgccctc aacttcctgc acgagagggg gatcatctac agggacctga
     1321 agctggacaa cgtcctcctg gatgcggacg ggcacatcaa gctcacagac tacggcatgt
     1381 gcaaggaagg cctgggccct ggtgacacaa cgagcacttt ctgcggaacc ccgaattaca
     1441 tcgcccccga aatcctgcgg ggagaggagt acgggttcag cgtggactgg tgggcgctgg
     1501 gagtcctcat gtttgagatg atggccgggc gctccccgtt cgacatcatc accgacaacc
     1561 cggacatgaa cacagaggac taccttttcc aagtgatcct ggagaagccc atccggatcc
     1621 cccggttcct gtccgtcaaa gcctcccatg ttttaaaagg atttttaaat aaggacccca
     1681 aagagaggct cggctgccgg ccacagactg gattttctga catcaagtcc cacgcgttct
     1741 tccgcagcat agactgggac ttgctggaga agaagcaggc gctccctcca ttccagccac
     1801 agatcacaga cgactacggt ctggacaact ttgacacaca gttcaccagc gagcccgtgc
     1861 agctgacccc agacgatgag gatgccataa agaggatcga ccagtcagag ttcgaaggct
     1921 ttgagtatat caacccatta ttgctgtcca ccgaggagtc ggtgtgccca actttcttgt
     1981 acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta
     2041 tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt
     2101 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
     2161 ttgcacaaga taaaaatata tcatcatgcc tcctctagac cagccaggac agaaatgcct
     2221 cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac
     2281 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
     2341 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     2401 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     2461 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     2521 agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat
     2581 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     2641 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
     2701 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     2761 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     2821 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     2881 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     2941 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     3001 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     3061 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     3121 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     3181 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     3241 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     3301 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat
     3361 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     3421 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3481 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3541 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3601 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3661 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3721 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3781 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3841 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3901 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3961 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4021 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4081 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     4141 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     4201 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     4261 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc
     4321 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     4381 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     4441 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     4501 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg
     4561 atgcct
//
