LOCUS       Exported                3615 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Gateway Donor vector containing ATG5 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    pDONR223_ATG5_WT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      17..605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     506..525
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     759..776
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      935..962
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      1054..1140
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1189..1211
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1203..1220
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1204..1220
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1236..1335
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2161..2260)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2277..2296)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2278..2296)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2301..2317)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2301..2317)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2748..3539
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
       61 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      601 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      661 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      721 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      781 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
      841 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc
      901 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
      961 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     1021 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     1081 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg
     1141 atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac
     1201 gttgtaaaac gacggccagt cttaagctcg ggccccaaat aatgatttta ttttgactga
     1261 tagtgacctg ttcgttgcaa caaattgatg agcaatgctt ttttataatg ccaactttgt
     1321 acaaaaaagt tggcatgaca gatgacaaag atgtgcttcg agatgtgtgg tttggacgaa
     1381 ttccaacttg tttcacgcta tatcaggatg agataactga aagggaagca gaaccatact
     1441 atttgctttt gccaagagta agttatttga cgttggtaac tgacaaagtg aaaaagcact
     1501 ttcagaaggt tatgagacaa gaagacatta gtgagatatg gtttgaatat gaaggcacac
     1561 cactgaaatg gcattatcca attggtttgc tatttgatct tcttgcatca agttcagctc
     1621 ttccttggaa catcacagta cattttaaga gttttccaga aaaagacctt ctgcactgtc
     1681 catctaagga tgcaattgaa gctcatttta tgtcatgtat gaaagaagct gatgctttaa
     1741 aacataaaag tcaagtaatc aatgaaatgc agaaaaaaga tcacaagcaa ctctggatgg
     1801 gattgcaaaa tgacagattt gaccagtttt gggccatcaa tcggaaactc atggaatatc
     1861 ctgcagaaga aaatggattt cgttatatcc cctttagaat atatcagaca acgactgaaa
     1921 gacctttcat tcagaagctg tttcgtcctg tggctgcaga tggacagttg cacacactag
     1981 gagatctcct caaagaagtt tgtccttctg ctattgatcc tgaagatggg gaaaaaaaga
     2041 atcaagtgat gattcatgga attgagccaa tgttggaaac acctctgcag tggctgagtg
     2101 aacatctgag ctacccggat aattttcttc atattagtat catcccacag ccaacagatt
     2161 gcccaacttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca
     2221 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct
     2281 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
     2341 atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc
     2401 aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga
     2461 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     2521 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     2581 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     2641 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     2701 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg
     2761 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     2821 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     2881 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     2941 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     3001 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     3061 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     3121 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     3181 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     3241 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     3301 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     3361 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     3421 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     3481 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac
     3541 cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt
     3601 cagaccccgt agaaa
//
