LOCUS Exported 11382 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION lentiviral expression vector for MS2-AID*Delta fusion protein with hygromycin B selectable marker. ACCESSION . VERSION . KEYWORDS pGH335_MS2-AID*Delta-Hygro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11382) AUTHORS Hess GT, Fresard L, Han K, Lee CH, Li A, Cimprich KA, Montgomery SB, Bassik MC TITLE Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. JOURNAL Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. PUBMED 27798611 REFERENCE 2 (bases 1 to 11382) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11382 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2687..3865 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2918..3856 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3813..3833 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" regulatory 3872..3881 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3881..4267 /codon_start=1 /product="bacteriophage MS2 coat protein" /label=MS2-N55K /note="binds with high affinity to a specific stem-loop structure in the viral RNA (Lim et al., 1994)" /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI VKAMQGLLKDGNPIPSAIAANSGIY" CDS 4322..4342 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 4958..5011 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 5015..6034 /codon_start=1 /product="hygromycin B phosphotransferase" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK " misc_feature 6074..6662 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6127..6147) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6545..6556) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(6665..6681) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6666..6682) /label=pBluescriptKS /note="For pBluescript vector" LTR 7187..7367 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(7393..7410) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 7399..7623 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 7669..8097 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7756..7775) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7966..7987 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(8106..8126) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 8111..8440 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 8291..8426 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8353..8372 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 8488..8535 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 8554..8928 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 9058..9179 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9095..9114) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9149..9168 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(9228..9244) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9228..9244) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(9241..9263) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 9252..9268 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(9276..9306) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9321..9342 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(9459..9476) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9630..10218) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9710..9729) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(10389..11249) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11012..11031 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(11250..11354) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta gctagctgca aagatggata aagttttaaa cagagaggaa 2641 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg 2701 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa 2761 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg 2821 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa 2881 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg 2941 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct 3001 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc 3061 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg 3121 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct 3181 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt 3241 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg 3301 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag 3361 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc 3421 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga 3481 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg 3541 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc 3601 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt 3661 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac 3721 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt 3781 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt 3841 ttttcttcca tttcaggtgt cgtgacgtac ggccaccatg gcttcaaact ttactcagtt 3901 cgtgctcgtg gacaatggtg ggacagggga tgtgacagtg gctccttcta atttcgctaa 3961 tggggtggca gagtggatca gctccaactc acggagccag gcctacaagg tgacatgcag 4021 cgtcaggcag tctagtgccc agaagagaaa gtataccatc aaggtggagg tccccaaagt 4081 ggctacccag acagtgggcg gagtcgaact gcctgtcgcc gcttggaggt cctacctgaa 4141 catggagctc actatcccaa ttttcgctac caattctgac tgtgaactca tcgtgaaggc 4201 aatgcagggg ctcctcaaag acggtaatcc tatcccttcc gccatcgccg ctaactcagg 4261 tatctacagc gctggaggag gtggaagcgg aggaggagga agcggaggag gaggtagcgg 4321 acctaagaaa aagaggaagg tggcggccgc tggatccatg gacagcctct tgatgaaccg 4381 gagggagttt ctttaccaat tcaaaaatgt ccgctgggct aagggtcggc gtgagaccta 4441 cctgtgctac gtagtgaaga ggcgtgacag tgctacatcc ttttcactgg actttggtta 4501 tcttcgcaat aagaacggct gccacgtgga attgctcttc ctccgctaca tctcggactg 4561 ggacctagac cctggccgct gctaccgcgt cacctggttc atctcctgga gcccctgcta 4621 cgactgtgcc cgacatgtgg ccgactttct gcgagggaac cccaacctca gtctgaggat 4681 cttcaccgcg cgcctctact tctgtgagga ccgcaaggct gagcccgagg ggctgcggcg 4741 gctgcaccgc gccggggtgc aaatagccat catgaccttc aaagattatt tttactgctg 4801 gaatactttt gtagaaaacc acggaagaac tttcaaagcc tgggaagggc tgcatgaaaa 4861 ttcagttcgt ctctccagac agcttcggcg catccttttg cccctgtatg aggttgatga 4921 cttacgagac gcatttcgta cttgtacagg cagtggagag ggcagaggaa gtctgctaac 4981 atgcggtgac gtcgaggaga atcctggccc aaccatgaaa aagcctgaac tcaccgctac 5041 ctctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc 5101 cgagggcgaa gaatctcggg ctttcagctt cgatgtggga gggcgtggat atgtcctgcg 5161 ggtgaatagc tgcgccgatg gtttctacaa agatcgctat gtttatcggc actttgcatc 5221 cgccgctctc cctattcccg aagtgcttga cattggggag ttcagcgaga gcctgaccta 5281 ttgcatctcc cgccgtgcac agggtgtcac cttgcaagac ctgcctgaaa ccgaactgcc 5341 cgctgttctc cagcccgtcg ccgaggccat ggatgccatc gctgccgccg atcttagcca 5401 gaccagcggg ttcggcccat tcggacctca aggaatcggt caatacacta catggcgcga 5461 tttcatctgc gctattgctg atccccatgt gtatcactgg caaactgtga tggacgacac 5521 cgtcagtgcc tccgtcgccc aggctctcga tgagctgatg ctttgggccg aggactgccc 5581 cgaagtccgg cacctcgtgc acgccgattt cggctccaac aatgtcctga ccgacaatgg 5641 ccgcataaca gccgtcattg actggagcga ggccatgttc ggggattccc aatacgaggt 5701 cgccaacatc ttcttctgga ggccctggtt ggcttgtatg gagcagcaga cccgctactt 5761 cgagcggagg catcccgagc ttgcaggatc tcctcggctc cgggcttata tgctccgcat 5821 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc 5881 tcagggtcgc tgcgacgcaa tcgtccggtc cggagccggg actgtcgggc gtacacaaat 5941 cgcccgcaga agcgctgccg tctggaccga tggctgtgtg gaagtgctcg ccgatagtgg 6001 aaacagacgc cccagcactc gtcctagggc aaaggatctg cagtaatgag aattcgatat 6061 caagcttatc ggtaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 6121 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 6181 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 6241 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 6301 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 6361 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 6421 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt 6481 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 6541 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 6601 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc 6661 gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac 6721 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt 6781 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag 6841 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga 6901 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac 6961 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt 7021 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt 7081 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg 7141 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt 7201 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 7261 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 7321 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa 7381 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc 7441 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg 7501 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg 7561 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta 7621 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta 7681 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 7741 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 7801 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc 7861 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat 7921 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 7981 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc 8041 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat 8101 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag 8161 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 8221 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 8281 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 8341 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa 8401 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat 8461 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc 8521 atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg 8581 gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc 8641 tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc 8701 atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc 8761 ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc 8821 tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc 8881 gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga 8941 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 9001 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac 9061 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 9121 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 9181 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt 9241 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 9301 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 9361 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 9421 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 9481 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 9541 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 9601 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 9661 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 9721 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 9781 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 9841 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 9901 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 9961 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 10021 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 10081 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 10141 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 10201 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 10261 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 10321 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 10381 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 10441 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 10501 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 10561 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 10621 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 10681 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 10741 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 10801 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 10861 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 10921 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 10981 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 11041 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 11101 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 11161 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 11221 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 11281 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 11341 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ac //