LOCUS       Exported                4548 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Gateway Donor vector containing CRY1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    pDONR223_CRY1_WT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      38..626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     527..546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     780..797
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      956..983
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      1075..1161
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1210..1232
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1224..1241
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1225..1241
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1257..1356
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3115..3214)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3231..3250)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3232..3250)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3255..3271)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3255..3271)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3702..4493
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
       61 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
      121 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
      181 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
      241 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
      301 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
      361 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
      421 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
      481 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
      541 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
      601 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
      661 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
      721 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
      781 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
      841 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
      901 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
      961 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     1021 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     1081 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     1141 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat
     1201 ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc gggccccaaa
     1261 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct
     1321 tttttataat gccaactttg tacaaaaaag ttggcatggg ggtgaacgcc gtgcactggt
     1381 tccgaaaggg gctccggctc cacgacaacc ccgccctgaa ggagtgcatt cagggcgccg
     1441 acaccatccg ctgcgtctac atcctggacc cctggttcgc cggctcctcc aatgtgggca
     1501 tcaacaggtg gcgatttttg cttcagtgtc ttgaggatct tgatgccaat ctacgaaaat
     1561 taaactcccg tctgtttgtg attcgtggac aaccagcaga tgtgtttccc aggcttttca
     1621 aggaatggaa cattactaaa ctttcaattg agtatgattc tgagcccttt ggaaaggaac
     1681 gagacgcagc tattaagaaa ctggcaactg aagctggagt agaagtcatt gtaagaattt
     1741 cacatacatt atatgaccta gacaagatca tagaactcaa tggtggacaa ccgcctctaa
     1801 cttataaaag attccagact ctcatcagca aaatggaacc actagagata ccagtagaga
     1861 caattacttc agaagtgata gaaaagtgca caactcctct gtctgatgac catgatgaga
     1921 aatatggagt cccttcactg gaagagctag gttttgatac agatggctta tcctctgcag
     1981 tgtggccagg cggagaaact gaagcactta ctcgtttgga aaggcatttg gaaagaaaag
     2041 cttgggtggc aaattttgaa agacctcgaa tgaatgcgaa ttctctgctt gcaagcccta
     2101 ctggacttag tccttatctc cgatttggtt gtttgtcatg tcgactgttt tacttcaaac
     2161 taacagatct ctacaaaaag gtaaagaaga acagttcccc tcccctttcc ctttatgggc
     2221 aactgttatg gcgtgaattt ttctatacag cagcaacaaa taatccacgc tttgataaaa
     2281 tggaaggaaa ccctatctgt gttcagattc cttgggataa aaatcctgag gctttagcca
     2341 aatgggcgga aggccggaca ggctttccat ggattgatgc catcatgaca cagcttcgtc
     2401 aggagggttg gattcatcat ctagccaggc atgcagttgc ttgcttcctg acacgagggg
     2461 acctgtggat tagttgggaa gaaggaatga aggtatttga agaattattg cttgatgcag
     2521 attggagcat aaatgctgga agttggatgt ggctgtcttg tagttccttt tttcaacagt
     2581 tttttcactg ctattgccct gttggttttg gtaggagaac agatcccaat ggagactata
     2641 tcaggcgtta tttgcctgtc ctaagaggct tccctgcaaa atatatctat gatccctgga
     2701 atgcaccaga aggtatccaa aaggtagcca aatgtttgat aggagttaat tatcctaaac
     2761 caatggtgaa ccatgctgag gcaagccgtt tgaatatcga aaggatgaaa cagatctatc
     2821 agcagctttc acgatataga ggactaggtc ttctggcatc agtaccttct aatcctaatg
     2881 ggaatggagg cttcatggga tattctgcag aaaatatccc aggttgtagc agcagtggaa
     2941 gttgctctca agggagtggt attttacact atgctcatgg cgacagtcag caaactcacc
     3001 tgttgaagca aggaagaagc tccatgggca ctggtctcag tggtgggaaa cgtcctagtc
     3061 aggaagagga cacacagagt attggtccta aagtccagag acagagcact aattacccaa
     3121 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
     3181 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
     3241 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     3301 gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca
     3361 gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga
     3421 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     3481 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     3541 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     3601 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     3661 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg
     3721 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     3781 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     3841 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     3901 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     3961 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     4021 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     4081 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     4141 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     4201 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     4261 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     4321 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     4381 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     4441 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga
     4501 gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccact
//
