LOCUS Exported 831 bp ds-DNA linear SYN 12-JUN-2021 DEFINITION individual 6N barcode to make barcoded cell line variants. ACCESSION . VERSION . KEYWORDS MSCV-6NBC-GFP-PGK-Puro-Barcode46 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 831) AUTHORS Gruner BM, Schulze CJ, Yang D, Ogasawara D, Dix MM, Rogers ZN, Chuang CH, McFarland CD, Chiou SH, Brown JM, Cravatt BF, Bogyo M, Winslow MM TITLE An in vivo multiplexed small-molecule screening platform. JOURNAL Nat Methods. 2016 Sep 12. doi: 10.1038/nmeth.3992. PUBMED 27617390 REFERENCE 2 (bases 1 to 831) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..831 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 50..766 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(95..116) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(356..375) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 703..724 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 cagccctcac tccttctcta ggcgccggaa ttagatccgg ggttccacca tggtgagcaa 61 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa 121 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac 181 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac 241 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt 301 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga 361 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat 421 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta 481 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt 541 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca 601 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac 661 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt 721 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtagc ggagaattct 781 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta g //