LOCUS Exported 4526 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway Donor vector containing MAP3K7 , part of the Target Accelerator Plasmid Collection.. ACCESSION . VERSION . KEYWORDS pDONR223_MAP3K7_WT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4526) AUTHORS Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn WC TITLE Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. JOURNAL Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4. PUBMED 27147599 REFERENCE 2 (bases 1 to 4526) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4526 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 43..65 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 57..74 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 58..74 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 90..189 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS complement(1007..1018) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" protein_bind complement(1926..2025) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2042..2061) /label=T7 /note="T7 promoter, forward primer" promoter complement(2043..2061) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2066..2082) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2066..2082) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 2513..3304 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 3397..3985 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3886..3905 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 4139..4156 /label=L4440 /note="L4440 vector, forward primer" terminator 4315..4342 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 4434..4520 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta 61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga 121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa 181 aagttggcat gtctacagcc tctgccgcct cctcctcctc ctcgtcttcg gccggtgaga 241 tgatcgaagc cccttcccag gtcctcaact ttgaagagat cgactacaag gagatcgagg 301 tggaagaggt tgttggaaga ggagcctttg gagttgtttg caaagctaag tggagagcaa 361 aagatgttgc tattaaacaa atagaaagtg aatctgagag gaaagcgttt attgtagagc 421 ttcggcagtt atcccgtgtg aaccatccta atattgtaaa gctttatgga gcctgcttga 481 atccagtgtg tcttgtgatg gaatatgctg aagggggctc tttatataat gtgctgcatg 541 gtgctgaacc attgccatat tatactgctg cccacgcaat gagttggtgt ttacagtgtt 601 cccaaggagt ggcttatctt cacagcatgc aacccaaagc gctaattcac agggacctga 661 aaccaccaaa cttactgctg gttgcagggg ggacagttct aaaaatttgt gattttggta 721 cagcctgtga cattcagaca cacatgacca ataacaaggg gagtgctgct tggatggcac 781 ctgaagtttt tgaaggtagt aattacagtg aaaaatgtga cgtcttcagc tggggtatta 841 ttctttggga agtgataacg cgtcggaaac cctttgatga gattggtggc ccagctttcc 901 gaatcatgtg ggctgttcat aatggtactc gaccaccact gataaaaaat ttacctaagc 961 ccattgagag cctgatgact cgttgttggt ctaaagatcc ttcccagcgc ccttcaatgg 1021 aggaaattgt gaaaataatg actcacttga tgcggtactt tccaggagca gatgagccat 1081 tacagtatcc ttgtcagtat tcagatgaag gacagagcaa ctctgccacc agtacaggct 1141 cattcatgga cattgcttct acaaatacga gtaacaaaag tgacactaat atggagcaag 1201 ttcctgccac aaatgatact attaagcgct tagaatcaaa attgttgaaa aatcaggcaa 1261 agcaacagag tgaatctgga cgtttaagct tgggagcctc ccgtgggagc agtgtggaga 1321 gcttgccccc aacctctgag ggcaagagga tgagtgctga catgtctgaa atagaagcta 1381 ggatcgccgc aaccacaggc aacggacagc caagacgtag atccatccaa gacttgactg 1441 taactggaac agaacctggt caggtgagca gtaggtcatc cagtcccagt gtcagaatga 1501 ttactacctc aggaccaacc tcagaaaagc caactcgaag tcatccatgg acccctgatg 1561 attccacaga taccaatgga tcagataact ccatcccaat ggcttatctt acactggatc 1621 accaactaca gcctctagca ccgtgcccaa actccaaaga atctatggca gtgtttgaac 1681 agcattgtaa aatggcacaa gaatatatga aagttcaaac agaaattgca ttgttattac 1741 agagaaagca agaactagtt gcagaactgg accaggatga aaaggaccag caaaatacat 1801 ctcgcctggt acaggaacat aaaaagcttt tagatgaaaa caaaagcctt tctacttact 1861 accagcaatg caaaaaacaa ctagaggtca tcagaagtca gcagcagaaa cgacaaggca 1921 cttcatccca actttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg 1981 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat 2041 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc 2101 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagac 2161 cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt gacgcacacc 2221 gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta 2281 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg 2341 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat 2401 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag 2461 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca ttatgaggga 2521 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca 2581 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa 2641 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg 2701 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct 2761 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc 2821 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga 2881 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt 2941 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt 3001 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga 3061 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc 3121 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt 3181 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc 3241 agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa 3301 ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg 3361 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 3421 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 3481 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 3541 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 3601 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 3661 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 3721 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 3781 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 3841 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 3901 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 3961 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 4021 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 4081 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 4141 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg 4201 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga 4261 gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg 4321 ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct 4381 gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc 4441 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac 4501 tgagcctttc gttttatttg atgcct //