LOCUS       Exported                4077 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Gateway Donor vector containing PDPK1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    pDONR223_PDPK1_WT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1477..1576)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1593..1612)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1594..1612)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1617..1633)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1617..1633)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2064..2855
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2948..3536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3437..3456
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3690..3707
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      3866..3893
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      3985..4071
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat ggccaggacc accagccagc tgtatgacgc cgtgcccatc cagtccagcg
      241 tggtgttatg ttcctgccca tccccatcaa tggtgaggac ccagactgag tccagcacgc
      301 cccctggcat tcctggtggc agcaggcagg gccccgccat ggacggcact gcagccgagc
      361 ctcggcccgg cgccggctcc ctgcagcatg cccagcctcc gccgcagcct cggaagaagc
      421 ggcctgagga cttcaagttt gggaaaatcc ttggggaagg ctctttttcc acggttgtcc
      481 tggctcgaga actggcaacc tccagagaat atgcgaccag ggccaactca ttcgtgggaa
      541 cagcgcagta cgtttctcca gagctgctca cggagaagtc cgcctgtaag agttcagacc
      601 tttgggctct tggatgcata atataccagc ttgtggcagg actcccacca ttccgagctg
      661 gaaacgagta tcttatattt cagaagatca ttaagttgga atatgacttt ccagaaaaat
      721 tcttccctaa ggcaagagac ctcgtggaga aacttttggt tttagatgcc acaaagcggt
      781 taggctgtga ggaaatggaa ggatacggac ctcttaaagc acacccgttc ttcgagtccg
      841 tcacgtggga gaacctgcac cagcagacgc ctccgaagct caccgcttac ctgccggcta
      901 tgtcggaaga cgacgaggac tgctatggca attatgacaa tctcctgagc cagtttggct
      961 gcatgcaggt gtcttcgtcc tcctcctcac actccctgtc agcctccgac acgggcctgc
     1021 cccagaggtc aggcagcaac atagagcagt acattcacga tctggactcg aactcctttg
     1081 aactggactt acagttttcc gaagatgaga agaggttgtt gttggagaag caggctggcg
     1141 gaaacccttg gcaccagttt gtagaaaata atttaatact aaagatgggc ccagtggata
     1201 agcggaaggg tttatttgca agacgacgac agctgttgct cacagaagga ccacatttat
     1261 attatgtgga tcctgtcaac aaagttctga aaggtgaaat tccttggtca caagaacttc
     1321 gaccagaggc caagaatttt aaaactttct ttgtccacac gcctaacagg acgtattatc
     1381 tgatggaccc cagcgggaac gcacacaagt ggtgcaggaa gatccaggag gtttggaggc
     1441 agcgatacca gagccacccg gacgccgctg tgcagtgccc aactttcttg tacaaagttg
     1501 gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
     1561 ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca
     1621 tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag
     1681 ataaaaatat atcatcatgc ctcctctaga ccagccagga cagaaatgcc tcgacttcgc
     1741 tgctacccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt
     1801 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     1861 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     1921 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     1981 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     2041 gccctaaaac aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac
     2101 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     2161 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     2221 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     2281 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     2341 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     2401 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     2461 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     2521 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     2581 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     2641 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     2701 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     2761 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     2821 tgaaaggcga gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat
     2881 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2941 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3001 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3061 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3121 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3181 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3241 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3301 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3361 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3421 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3481 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3541 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3601 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3661 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     3721 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     3781 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
     3841 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     3901 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     3961 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     4021 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcct
//
