LOCUS Exported 6555 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway Donor vector containing ERBB2, part of the Target Accelerator Plasmid Collection.. ACCESSION . VERSION . KEYWORDS pDONR223_ERBB2_WT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6555) AUTHORS Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn WC TITLE Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. JOURNAL Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4. PUBMED 27147599 REFERENCE 2 (bases 1 to 6555) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6555 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 57..645 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 546..565 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 799..816 /label=L4440 /note="L4440 vector, forward primer" terminator 975..1002 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 1094..1180 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 1229..1251 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 1243..1260 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 1244..1260 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 1276..1375 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(5141..5240) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(5257..5276) /label=T7 /note="T7 promoter, forward primer" promoter complement(5258..5276) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(5281..5297) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5281..5297) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 5728..6519 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" ORIGIN 1 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 61 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 121 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 181 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag 241 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 301 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 361 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 421 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 481 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 541 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 601 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 661 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 721 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 781 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc 841 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 901 gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag 961 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg 1021 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat 1081 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac 1141 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact 1201 ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt 1261 cttaagctcg ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa 1321 caaattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagt tggcaccatg 1381 gagctggcgg ccttgtgccg ctgggggctc ctcctcgccc tcttgccccc cggagccgcg 1441 agcacccaag tgtgcaccgg cacagacatg aagctgcggc tccctgccag tcccgagacc 1501 cacctggaca tgctccgcca cctctaccag ggctgccagg tggtgcaggg aaacctggaa 1561 ctcacctacc tgcccaccaa tgccagcctg tccttcctgc aggatatcca ggaggtgcag 1621 ggctacgtgc tcatcgctca caaccaagtg aggcaggtcc cactgcagag gctgcggatt 1681 gtgcgaggca cccagctctt tgaggacaac tatgccctgg ccgtgctaga caatggagac 1741 ccgctgaaca ataccacccc tgtcacaggg gcctccccag gaggcctgcg ggagctgcag 1801 cttcgaagcc tcacagagat cttgaaagga ggggtcttga tccagcggaa cccccagctc 1861 tgctaccagg acacgatttt gtggaaggac atcttccaca agaacaacca gctggctctc 1921 acactgatag acaccaaccg ctctcgggcc tgccacccct gttctccgat gtgtaagggc 1981 tcccgctgct ggggagagag ttctgaggat tgtcagagcc tgacgcgcac tgtctgtgcc 2041 ggtggctgtg cccgctgcaa ggggccactg cccactgact gctgccatga gcagtgtgct 2101 gccggctgca cgggccccaa gcactctgac tgcctggcct gcctccactt caaccacagt 2161 ggcatctgtg agctgcactg cccagccctg gtcacctaca acacagacac gtttgagtcc 2221 atgcccaatc ccgagggccg gtatacattc ggcgccagct gtgtgactgc ctgtccctac 2281 aactaccttt ctacggacgt gggatcctgc accctcgtct gccccctgca caaccaagag 2341 gtgacagcag aggatggaac acagcggtgt gagaagtgca gcaagccctg tgcccgagtg 2401 tgctatggtc tgggcatgga gcacttgcga gaggtgaggg cagttaccag tgccaatatc 2461 caggagtttg ctggctgcaa gaagatcttt gggagcctgg catttctgcc ggagagcttt 2521 gatggggacc cagcctccaa cactgccccg ctccagccag agcagctcca agtgtttgag 2581 actctggaag agatcacagg ttacctatac atctcagcat ggccggacag cctgcctgac 2641 ctcagcgtct tccagaacct gcaagtaatc cggggacgaa ttctgcacaa tggcgcctac 2701 tcgctgaccc tgcaagggct gggcatcagc tggctggggc tgcgctcact gagggaactg 2761 ggcagtggac tggccctcat ccaccataac acccacctct gcttcgtgca cacggtgccc 2821 tgggaccagc tctttcggaa cccgcaccaa gctctgctcc acactgccaa ccggccagag 2881 gacgagtgtg tgggcgaggg cctggcctgc caccagctgt gcgcccgagg gcactgctgg 2941 ggtccagggc ccacccagtg tgtcaactgc agccagttcc ttcggggcca ggagtgcgtg 3001 gaggaatgcc gagtactgca ggggctcccc agggagtatg tgaatgccag gcactgtttg 3061 ccgtgccacc ctgagtgtca gccccagaat ggctcagtga cctgttttgg accggaggct 3121 gaccagtgtg tggcctgtgc ccactataag gaccctccct tctgcgtggc ccgctgcccc 3181 agcggtgtga aacctgacct ctcctacatg cccatctgga agtttccaga tgaggagggc 3241 gcatgccagc cttgccccat caactgcacc cactcctgtg tggacctgga tgacaagggc 3301 tgccccgccg agcagagagc cagccctctg acgtccatca tctctgcggt ggttggcatt 3361 ctgctggtcg tggtcttggg ggtggtcttt gggatcctca tcaagcgacg gcagcagaag 3421 atccggaagt acacgatgcg gagactgctg caggaaacgg agctggtgga gccgctgaca 3481 cctagcggag cgatgcccaa ccaggcgcag atgcggatcc tgaaagagac ggagctgagg 3541 aaggtgaagg tgcttggatc tggcgctttt ggcacagtct acaagggcat ctggatccct 3601 gatggggaga atgtgaaaat tccagtggcc atcaaagtgt tgagggaaaa cacatccccc 3661 aaagccaaca aagaaatctt agacgaagca tacgtgatgg ctggtgtggg ctccccatat 3721 gtctcccgcc ttctgggcat ctgcctgaca tccacggtgc agctggtgac acagcttatg 3781 ccctatggct gcctcttaga ccatgtccgg gaaaaccgcg gacgcctggg ctcccaggac 3841 ctgctgaact ggtgtatgca gattgccaag gggatgagct acctggagga tgtgcggctc 3901 gtacacaggg acttggccgc tcggaacgtg ctggtcaaga gtcccaacca tgtcaaaatt 3961 acagacttcg ggctggctcg gctgctggac attgacgaga cagagtacca tgcagatggg 4021 ggcaaggtgc ccatcaagtg gatggcgctg gagtccattc tccgccggcg gttcacccac 4081 cagagtgatg tgtggagtta tggtgtgact gtgtgggagc tgatgacttt tggggccaaa 4141 ccttacgatg ggatcccagc ccgggagatc cctgacctgc tggaaaaggg ggagcggctg 4201 ccccagcccc ccatctgcac cattgatgtc tacatgatca tggtcaaatg ttggatgatt 4261 gactctgaat gtcggccaag attccgggag ttggtgtctg aattctcccg catggccagg 4321 gacccccagc gctttgtggt catccagaat gaggacttgg gcccagccag tcccttggac 4381 agcaccttct accgctcact gctggaggac gatgacatgg gggacctggt ggatgctgag 4441 gagtatctgg taccccagca gggcttcttc tgtccagacc ctgccccggg cgctgggggc 4501 atggtccacc acaggcaccg cagctcatct accaggagtg gcggtgggga cctgacacta 4561 gggctggagc cctctgaaga ggaggccccc aggtctccac tggcaccctc cgaaggggct 4621 ggctccgatg tatttgatgg tgacctggga atgggggcag ccaaggggct gcaaagcctc 4681 cccacacatg accccagccc tctacagcgg tacagtgagg accccacagt acccctgccc 4741 tctgagactg atggctacgt tgcccccctg acctgcagcc cccagcctga atatgtgaac 4801 cagccagatg ttcggcccca gcccccttcg ccccgagagg gccctctgcc tgctgcccga 4861 cctgctggtg ccactctgga aaggcccaag actctctccc cagggaagaa tggggtcgtc 4921 aaagacgttt ttgcctttgg gggtgccgtg gagaaccccg agtacttgac accccaggga 4981 ggagctgccc ctcagcccca ccctcctcct gccttcagcc cagccttcga caacctctat 5041 tactgggacc aggacccacc agagcggggg gctccaccca gcaccttcaa agggacacct 5101 acggcagaga acccagagta cctgggtctg gacgtgccag tgccaacttt cttgtacaaa 5161 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt 5221 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg 5281 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca 5341 caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact 5401 tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc 5461 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc 5521 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg 5581 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg 5641 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc 5701 agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact 5761 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta 5821 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg 5881 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg 5941 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt 6001 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa 6061 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg 6121 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag 6181 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg 6241 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc 6301 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg 6361 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat 6421 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac 6481 tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca 6541 aaatccctta acgtg //