LOCUS Exported 872 bp ds-DNA linear SYN 26-MAY-2021 DEFINITION Introduces DSB at A08 locus in Y. lipolytica. ACCESSION . VERSION . KEYWORDS pCRISPRyl_A08 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 872) AUTHORS Schwartz C, Shabbir-Hussain M, Frogue K, Blenner M, Wheeldon I TITLE Standardized markerless gene integration for pathway engineering in Yarrowia lipolytica. JOURNAL ACS Synth Biol. 2016 Dec 19. PUBMED 27989123 REFERENCE 2 (bases 1 to 872) AUTHORS . TITLE Direct Submission JOURNAL Exported May 26, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..872 /organism="synthetic DNA construct" /mol_type="other DNA" misc_RNA 518..593 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" primer_bind 643..661 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtccccag 61 ttgcaaaagt tgacacaact ctagatctgc ttccaaatat agaatcataa caagggttag 121 ggtgtgatta tataatattg gtcttaattg atgtgctagg gctttaaaag ttggttaaaa 181 taacgctcta atgccttttt aatatattgt ctttttcaaa atctcaaatc ggacacttct 241 tcgtgtatga gactccattt tttggctccg tcacgtgata tgtattatca gctatagtgg 301 tgtaaacaaa gttttttact agctgtaatg gcattttgtc ggagtggtaa atcgccttct 361 tgttgtgcgt tcgagttctg gactctgcac tgggctactt tgaaaaatac ctctaatgcg 421 ccgatggttt agtggtaaaa tccatcgttg ccatcgatgg gcccccggtt cgattccggg 481 tcggcgcagg ttgacgtgag tacggcattg attcagagtt ttagagctag aaatagcaag 541 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt 601 tacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg 661 cccagatcct ctagagtcga agcggccgct atgtctgata aaaggatgta acataggcaa 721 gctgctcgtg agtgttgagt acgaacctta gatccaaatc acccgcaccc acggatatac 781 tngcttgaat atacagtagt atgcggccgc ttcgacacca tatcatataa aactaacaat 841 gcattgaaat taatacgaag actacccgtt gc //