LOCUS Exported 4317 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway Donor vector containing LCK, part of the Target Accelerator Plasmid Collection.. ACCESSION . VERSION . KEYWORDS pDONR223_LCK_WT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4317) AUTHORS Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn WC TITLE Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. JOURNAL Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4. PUBMED 27147599 REFERENCE 2 (bases 1 to 4317) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4317 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 387..406 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 640..657 /label=L4440 /note="L4440 vector, forward primer" terminator 816..843 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 935..1021 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 1070..1092 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 1084..1101 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 1085..1101 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 1117..1216 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2744..2843) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2860..2879) /label=T7 /note="T7 promoter, forward primer" promoter complement(2861..2879) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2884..2900) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2884..2900) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 3331..4122 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 4215..486 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg 61 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 121 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 181 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 241 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 301 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 361 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 421 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 481 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 541 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag 601 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 661 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 721 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg 781 cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct 841 tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc 901 gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca 961 ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt 1021 gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga 1081 cgttgtaaaa cgacggccag tcttaagctc gggccccaaa taatgatttt attttgactg 1141 atagtgacct gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg 1201 tacaaaaaag ttggcaccat gggctgtggc tgcagctcac acccggaaga tgactggatg 1261 gaaaacatcg atgtgtgtga gaactgccat tatcccatag tcccactgga tggcaagggc 1321 acgctgctca tccgaaatgg ctctgaggtg cgggacccac tgattaccta cgaaggctcc 1381 aatccgccgg cttccccact gcaagacaac ctggttatcg ctctgcacag ctatgagccc 1441 tctcacgacg gagatctggg ctttgagaag ggggaacagc tccgcatcct ggagcagagc 1501 ggcgagtggt ggaaggcgca gtccctgacc acgggccagg aaggcttcat ccccttcaat 1561 tttgtggcca aagcgaacag cctggagccc gaaccctggt tcttcaagaa cctgagccgc 1621 aaggacgcgg agcggcagct cctggcgccc gggaacactc acggctcctt cctcatccgg 1681 gagagcgaga gcaccgcggg atcgttttca ctgtcggtcc gggacttcga ccagaaccag 1741 ggagaggtgg tgaaacatta caagatccgt aatctggaca acggtggctt ctacatctcc 1801 cctcgaatca cttttcccgg cctgcatgaa ctggtccgcc attacaccaa tgcttcagat 1861 gggctgtgca cacggttgag ccgcccctgc cagacccaga agccccagaa gccgtggtgg 1921 gaggacgagt gggaggttcc cagggagacg ctgaagctgg tggagcggct gggggctgga 1981 cagttcgggg aggtgtggat ggggtactac aacgggcaca cgaaggtggc ggtgaagagc 2041 ctgaagcagg gcagcatgtc cccggacgcc ttcctggccg aggccaacct catgaagcag 2101 ctgcaacacc agcggctggt tcggctctac gctgtggtca cccaggagcc catctacatc 2161 atcactgaat acatggagaa tgggagtcta gtggattttc tcaagacccc ttcaggcatc 2221 aagttgacca tcaacaaact cctggacatg gcagcccaaa ttgcagaagg catggcattc 2281 attgaagagc ggaattatat tcatcgtgac cttcgggctg ccaacattct ggtgtctgac 2341 accctgagct gcaagattgc agactttggc ctagcacgcc tcattgagga caacgagtac 2401 acagccaggg agggggccaa gtttcccatt aagtggacag cgccagaagc cattaactac 2461 gggacattca ccatcaagtc agatgtgtgg tcttttggga tcctgctgac ggaaattgtc 2521 acccacggcc gcatccctta cccagggatg accaacccgg aggtgattca gaacctggag 2581 cgaggctacc gcatggtgcg ccctgacaac tgtccagagg agctgtacca actcatgagg 2641 ctgtgctgga aggagcgccc agaggaccgg cccacctttg actacctgcg cagtgtgctg 2701 gaggacttct tcacggccac agagggccag taccagcctc agcctccaac tttcttgtac 2761 aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc 2821 agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac 2881 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt 2941 gcacaagata aaaatatatc atcatgcctc ctctagacca gccaggacag aaatgcctcg 3001 acttcgctgc tacccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga 3061 acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca 3121 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc 3181 atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa 3241 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag 3301 ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc cgaagtatcg 3361 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc 3421 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg 3481 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt 3541 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt 3601 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga 3661 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat 3721 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg 3781 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta 3841 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg 3901 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac 3961 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct 4021 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc 4081 cactacgtga aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga 4141 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag 4201 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 4261 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tcttttt //