LOCUS Exported 793 bp ds-DNA linear SYN 07-SEP-2021 DEFINITION individual 6N barcode to make barcoded cell line variants. ACCESSION . VERSION . KEYWORDS MSCV-6NBC-GFP-PGK-Puro-Barcode70 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 793) AUTHORS Gruner BM, Schulze CJ, Yang D, Ogasawara D, Dix MM, Rogers ZN, Chuang CH, McFarland CD, Chiou SH, Brown JM, Cravatt BF, Bogyo M, Winslow MM TITLE An in vivo multiplexed small-molecule screening platform. JOURNAL Nat Methods. 2016 Sep 12. doi: 10.1038/nmeth.3992. PUBMED 27617390 REFERENCE 2 (bases 1 to 793) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 7, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..793 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 35..44 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 41..757 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(86..107) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(347..366) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 694..715 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 ctccttctct aggcgccgga attagatccg ccaagccacc atggtgagca agggcgagga 61 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa 121 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt 181 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta 241 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc 301 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta 361 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa 421 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa 481 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa 541 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac 601 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc 661 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc 721 cgccgggatc actctcggca tggacgagct gtacaagtag cggagaattc taccgggtag 781 gggaggcgct ttt //