LOCUS Exported 744 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Arabinose inducible E. coli codon optimized superfolder GFP with C-terminal His6 tag. ACCESSION . VERSION . KEYWORDS pBad-sfGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 744) AUTHORS Miyake-Stoner SJ, Refakis CA, Hammill JT, Lusic H, Hazen JL, Deiters A, Mehl RA TITLE Generating permissive site-specific unnatural aminoacyl-tRNA synthetases. JOURNAL Biochemistry. 2010 Mar 2;49(8):1667-77. doi: 10.1021/bi901947r. PUBMED 20082521 REFERENCE 2 (bases 1 to 744) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..744 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1..717 /codon_start=1 /product="GFP variant that folds robustly even when fused to poorly folded proteins (Pedelacq et al., 2006)" /label=superfolder GFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL EFVTAAGITHGMDELYK" CDS 724..741 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" ORIGIN 1 atggttagca aaggtgaaga actgtttacc ggcgttgtgc cgattctggt ggaactggat 61 ggtgatgtga atggccataa atttagcgtt cgtggcgaag gcgaaggtga tgcgaccaac 121 ggtaaactga ccctgaaatt tatttgcacc accggtaaac tgccggttcc gtggccgacc 181 ctggtgacca ccctgaccta tggcgttcag tgctttagcc gctatccgga tcatatgaaa 241 cgccatgatt tctttaaaag cgcgatgccg gaaggctatg tgcaggaacg taccattagc 301 ttcaaagatg atggcaccta taaaacccgt gcggaagtta aatttgaagg cgataccctg 361 gtgaaccgca ttgaactgaa aggtattgat tttaaagaag atggcaacat tctgggtcat 421 aaactggaat ataatttcaa cagccataat gtgtatatta ccgccgataa acagaaaaat 481 ggcatcaaag cgaactttaa aatccgtcac aacgtggaag atggtagcgt gcagctggcg 541 gatcattatc agcagaatac cccgattggt gatggcccgg tgctgctgcc ggataatcat 601 tatctgagca cccagagcgt tctgagcaaa gatccgaatg aaaaacgtga tcatatggtg 661 ctgctggaat ttgttaccgc cgcgggcatt acccacggta tggatgaact gtataaaggc 721 agccaccatc atcatcacca ttaa //