LOCUS       Exported                1000 bp ds-DNA     linear   SYN 30-MAY-2021
DEFINITION  Expresses GA-inducible VPR-Sp dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 30, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..18
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   69..131
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     CDS             complement(348..368)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(375..395)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(414..440)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 attaaccctc actaaaggga acaaaagctg gattaattaa gcggccgcat acgcgtatac
       61 tagattaacc ctagaaagat aatcatattg tgacgtacgt taaagataat catgcgtaaa
      121 attgacgcat gtgttttatc ggtctgtata tcgaggttta tttattaatt tgaatagata
      181 ttaagtttta ttatatttac acttacatac taataataaa ttcaacaaac aatttattta
      241 tgtttattta tttattaaaa aaaaacaaaa actcaaaatt tcttctataa agtaacaaaa
      301 cttttaacta gctcgagtca tccgttgctc ccactaccaa tgccgtccac tttccgcttt
      361 ttcttaggat cttccacctt gcgttttttc ttgggggagc cgctgcccag gctggcataa
      421 tcgggcacgt catagggata ggcgtcgcct cccagctgag acaggtcgat ccgtgtctcg
      481 tacaggccgg tgatgctctg gtggatcagg gtggcgtcca gcacctcttt ggtgctggtg
      541 tacctcttcc ggtcgatggt ggtgtcaaag tacttgaagg cggcaggggc tcccagattg
      601 gtcagggtaa acaggtggat gatattctcg gcctgctctc tgataggctt gtctctgtgc
      661 ttgttgtagg cgctcagcac cttgtccaga ttagcgtcgg ccaggatcac tctcttggag
      721 aactcgctga tctgctcgat gatctcgtcc aggtagtgtt tgtgctgttc cacaaacagc
      781 tgtttctgct cattatcctc gggggagccc ttcagcttct catagtggct ggccaggtac
      841 aggaagttca catatttgga gggcagggcc agttcgtttc ccttctgcag ttcgccggca
      901 gaggccagca ttctcttccg gccgttttcc agctcgaaca gggagtactt aggcagcttg
      961 atgatcaggt cctttttcac ttctttgtag cccttggctt
//
