LOCUS       Exported                 949 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  Expresses ABA-inducible KRAB-Sp dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA
            PGK-ABI-tagB...
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             169..768
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(169..188)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     665..685
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(859..879)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacgtgtgtt
       61 tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
      121 aaacacgatg ataatatggc cacaaccgga tccggagcta ccatgaccat gaccgagtac
      181 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
      241 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
      301 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
      361 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
      421 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
      481 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
      541 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
      601 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
      661 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
      721 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagt ttaaaccatg
      781 caagcttgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
      841 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
      901 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttg
//
