LOCUS       Exported                 864 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  bacterial expression of Bacillus anthracis protective antigen.
ACCESSION   .
VERSION     .
KEYWORDS    pET22b - PA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Rauch I, Tenthorey JL, Nichols RD, Al Moussawi K, Kang JJ, Kang C, 
            Kazmierczak BI, Vance RE
  TITLE     NAIP proteins are required for cytosolic detection of specific 
            bacterial ligands in vivo.
  JOURNAL   J Exp Med. 2016 May 2;213(5):657-65. doi: 10.1084/jem.20151809. Epub
            2016 Apr 4.
  PUBMED    27045008
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(19..36)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtcattatc ctatctcata
       61 gcctttttta gaaaagatta aaattttctt gatcccgttg gtactagtat ccccattctc
      121 actaggatta ataatagtgt tttctttagt aacagcatat acatttacct tataattggg
      181 attacttata tataacggta atttatcatt atatttttta aaatctataa atgtttttcc
      241 atcttgccgt aaactagaaa tattcaacat atcatatctg tcatttataa cttctttaag
      301 cccttcagta tcttcaattt ctacaatata acctgataat atttttctta tatccttatc
      361 aatatttaac aataatccct ctgttgacga attaattact tctctatgag cctccttaac
      421 tactgactca tccgccccaa ctgctatgtt atttctatca taatgaaaac gtttatctct
      481 tattaaaata ttcatttttg catttaattt gattttatct aatacagtat atatgttagt
      541 tgcgtttaat tccgctaact gattcttgat attttgagat gtttgttgat cgaaattaaa
      601 atcaaattcg gttatgtctt tcccttgata ttgtaagttt ccattcggtt cgttaaatcc
      661 aaatgctatt ttaagggctt cttttaatgt catatccggt ttagtcgttt ctaatggatc
      721 actaggatta accgccgcta tccgcctttc taccagattt aaatcttttc cattaaaaat
      781 gatacgtgca gttgtttctt gaatttgcgg taacacttca ctccagttcg agcctgtatc
      841 caccctcact cttccatttt caaa
//
