LOCUS Exported 14298 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Dosage control and tracing of reprogramming episomes. ACCESSION . VERSION . KEYWORDS pCXLE-hOCT3/4-shp53-F + mCherry-2A-puro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 14298) AUTHORS Schmitt CE, Morales BM, Schmitz EMH, Hawkins JS, Lizama CO, Zape JP, Hsiao EC, Zovein AC TITLE Fluorescent tagged episomals for stoichiometric induced pluripotent stem cell reprogramming. JOURNAL Stem Cell Res Ther. 2017 Jun 5;8(1):132. doi: 10.1186/s13287-017-0581-7. PUBMED 28583172 REFERENCE 2 (bases 1 to 14298) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..14298 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 4..383 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 385..661 /label=chicken beta-actin promoter intron 662..1678 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 1602..1624 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 1686..1705 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" misc_feature 2866..3454 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2919..2939) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3337..3348) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(3544..3563) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 3609..3664 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(3663..3682) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(4025..4041) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4025..4041) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4038..4060) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4049..4065 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4073..4103) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4118..4139 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." protein_bind complement(4228..4261) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." polyA_signal 4701..4925 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" CDS complement(4932..5528) /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(5015..5035) /label=Puro-F /note="Puromycin resistance gene, forward primer" CDS complement(5532..5585) /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS complement(5592..6299) /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(5867..5886) /label=mCherry-F /note="mCherry, forward primer" primer_bind 6136..6154 /label=mCherry-R /note="mCherry, reverse primer" primer_bind complement(6391..6415) /label=LNCX /note="Human CMV promoter, forward primer" promoter complement(6412..6615) /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind complement(6441..6461) /label=CMV-F /note="Human CMV immediate early promoter, forward primer" enhancer 6616..6919 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" CDS 7339..9264 /codon_start=1 /product="Epstein-Barr nuclear antigen 1, also known as EBNA-1" /label=EBNA1 /note="crucial for latent viral infection, and for episomal amplification of vectors containing the oriP origin (Okita et al., 2013)" /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE GEEGQE" protein_bind complement(9432..9465) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." rep_origin 9652..11441 /label=oriP /note="Epstein-Barr virus oriP replication origin (Yates et al., 2000)" promoter 11912..12225 /label=U6 promoter /note="RNA polymerase III promoter for mouse U6 snRNA (Das et al., 1988)" primer_bind 12144..12164 /label=mU6-F /note="Mouse U6 promoter, forward primer" primer_bind complement(12376..12393) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(12547..13135) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(12627..12646) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(13306..14166) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 13929..13948 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(14167..14271) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca 421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag 481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc 541 ggggcgggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc 721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc 781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga 841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg 901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg 961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg 1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt 1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca 1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg 1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc 1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg 1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc 1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac 1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga 1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc 1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt 1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct 1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgcccttca 1741 ccatggcggg acacctggct tcggatttcg ccttctcgcc ccctccaggt ggtggaggtg 1801 atgggccagg ggggccggag ccgggctggg ttgatcctcg gacctggcta agcttccaag 1861 gccctcctgg agggccagga atcgggccgg gggttgggcc aggctctgag gtgtggggga 1921 ttcccccatg ccccccgccg tatgagttct gtggggggat ggcgtactgt gggccccagg 1981 ttggagtggg gctagtgccc caaggcggct tggagacctc tcagcctgag ggcgaagcag 2041 gagtcggggt ggagagcaac tccgatgggg cctccccgga gccctgcacc gtcacccctg 2101 gtgccgtgaa gctggagaag gagaagctgg agcaaaaccc ggaggagtcc caggacatca 2161 aagctctgca gaaagaactc gagcaatttg ccaagctcct gaagcagaag aggatcaccc 2221 tgggatatac acaggccgat gtggggctca ccctgggggt tctatttggg aaggtattca 2281 gccaaacgac catctgccgc tttgaggctc tgcagcttag cttcaagaac atgtgtaagc 2341 tgcggccctt gctgcagaag tgggtggagg aagctgacaa caatgaaaat cttcaggaga 2401 tatgcaaagc agaaaccctc gtgcaggccc gaaagagaaa gcgaaccagt atcgagaacc 2461 gagtgagagg caacctggag aatttgttcc tgcagtgccc gaaacccaca ctgcagcaga 2521 tcagccacat cgcccagcag cttgggctcg agaaggatgt ggtccgagtg tggttctgta 2581 accggcgcca gaagggcaag cgatcaagca gcgactatgc acaacgagag gattttgagg 2641 ctgctgggtc tcctttctca gggggaccag tgtcctttcc tctggcccca gggccccatt 2701 ttggtacccc aggctatggg agccctcact tcactgcact gtactcctcg gtccctttcc 2761 ctgaggggga agcctttccc cctgtctctg tcaccactct gggctctccc atgcattcaa 2821 actgaggtaa gggcgaattc aagcttcggg gactagtcat atgataatca acctctggat 2881 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 2941 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 3001 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 3061 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 3121 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 3181 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 3241 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc 3301 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 3361 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag 3421 acgagtcgga tctccctttg ggccgcctcc ccgcatcggt aaattcactc ctcaggtgca 3481 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg 3541 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac 3601 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct 3661 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt 3721 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag 3781 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt 3841 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct 3901 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt 3961 catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat 4021 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 4081 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 4141 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatctcaa 4201 ttccgatcat attcaataac ccttaatata acttcgtata atgtatgcta tacgaagtta 4261 ttaggtctga agaggagttt acgtccagcc aagcttagga tcaattctca tgtttgacag 4321 cttatcatcg atggccggcc ggcgcccttg ctcaccatga ccggtggatc ccgggccctg 4381 gtgagcctgg cctctcctta cctcaggtcc gtgggaggct gggggctggg ctgccgccgc 4441 cgctgccacc tgtccacact gtttatctgg ctgagtggtt tctggcatgc cccgcagagg 4501 tgactgggac ctctgaggag ccgctgctgc cgcctcagtt cggaatcgct gcatcccagg 4561 ctggggatag acatggaaag ggtgcacctg ccggttctca gcgccttcat ggagcacagg 4621 aatgggaatg tagcctggtc ggagctgggg gtacacaggg tggccttccc tagcaggcgg 4681 cagcctagag ccctcccggg ccatagagcc caccgcatcc ccagcatgcc tgctattgtc 4741 ttcccaatcc tcccccttgc tgtcctgccc caccccaccc cccagaatag aatgacacct 4801 actcagacaa tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg gagtggcacc 4861 ttccagggtc aaggaaggca cgggggaggg gcaaacaaca gatggctggc aactagaagg 4921 cacaggaatt cttaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc 4981 acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc 5041 gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac tccggggagc 5101 acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac ggtggccagg 5161 aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg 5221 gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa caccgccccc 5281 gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac 5341 accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc 5401 tcgatgtggc ggtccggatc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg 5461 gcgagggtgc gtacggccct ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg 5521 tactcggttc gagggccggg attctcctcc acgtcaccgc atgttagaag acttcctctg 5581 ccctcccggt gcttgtacag ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt 5641 tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg 5701 acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt cttgacctca 5761 gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct tgatctcgcc cttcagggcg 5821 ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc ccatggtctt cttctgcatt 5881 acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat gaactcgccg 5941 tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg 6001 cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat gtcggcgggg 6061 tgcttcacgt aggccttgga gccgtacatg aactgagggg acaggatgtc ccaggcgaag 6121 ggcagggggc cacccttggt caccttcagc ttggcggtct gggtgccctc gtaggggcgg 6181 ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc catgtgcacc 6241 ttgaagcgca tgaactcctt gatgatggcc atgttatcct cctcgccctt gctcaccatc 6301 tcgagatctg agtccggtag cgctagcgat cctctagtag agtcggtgtc ttctatggag 6361 gtcaaaacag cgtggatggc gtctccaggc gatctgacgg ttcactaaac gagctctgct 6421 tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt 6481 acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat 6541 ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg 6601 ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg 6661 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac 6721 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta 6781 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac 6841 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt 6901 taccgtaagt tatgtaacgc ggaactccca agcttgcgat cgcatcgata agctgatcct 6961 cacaggccgc acccagcttt tcttccgttg ccccagtagc atctctgtct ggtgaccttg 7021 aagaggaaga ggaggggtcc cgagaatccc catccctacc gtccagcaaa aagggggacg 7081 aggaatttga ggcctggctt gaggctcagg acgcaaatct tgaggatgtt cagcgggagt 7141 tttccgggct gcgagtaatt ggtgatgagg acgaggatgg ttcggaggat ggggaatttt 7201 cagacctgga tctgtctgac agcgaccatg aaggggatga gggtgggggg gctgttggag 7261 ggggcaggag tctgcactcc ctgtattcac tgagcgtcgt ctaataaaga tgtctattga 7321 tctcttttag tgtgaatcat gtctgacgag gggccaggta caggacctgg aaatggccta 7381 ggagagaagg gagacacatc tggaccagaa ggctccggcg gcagtggacc tcaaagaaga 7441 gggggtgata accatggacg aggacgggga agaggacgag gacgaggagg cggaagacca 7501 ggagccccgg gcggctcagg atcagggcca agacatagag atggtgtccg gagaccccaa 7561 aaacgtccaa gttgcattgg ctgcaaaggg acccacggtg gaacaggagc aggagcagga 7621 gcgggagggg caggagcagg aggggcagga gcaggaggag gggcaggagc aggaggaggg 7681 gcaggagggg caggaggggc aggaggggca ggagcaggag gaggggcagg agcaggagga 7741 ggggcaggag gggcaggagg ggcaggagca ggaggagggg caggagcagg aggaggggca 7801 ggaggggcag gagcaggagg aggggcagga ggggcaggag gggcaggagc aggaggaggg 7861 gcaggagcag gaggaggggc aggaggggca ggagcaggag gaggggcagg aggggcagga 7921 ggggcaggag caggaggagg ggcaggagca ggaggggcag gaggggcagg aggggcagga 7981 gcaggagggg caggagcagg aggaggggca ggaggggcag gaggggcagg agcaggaggg 8041 gcaggagcag gaggggcagg agcaggaggg gcaggagcag gaggggcagg aggggcagga 8101 gcaggagggg caggaggggc aggagcagga ggggcaggag gggcaggagc aggaggaggg 8161 gcaggagggg caggagcagg aggaggggca ggaggggcag gagcaggagg ggcaggaggg 8221 gcaggagcag gaggggcagg aggggcagga gcaggagggg caggaggggc aggagcagga 8281 ggaggggcag gagcaggagg ggcaggagca ggaggtggag gccggggtcg aggaggcagt 8341 ggaggccggg gtcgaggagg tagtggaggc cggggtcgag gaggtagtgg aggccgccgg 8401 ggtagaggac gtgaaagagc caggggggga agtcgtgaaa gagccagggg gagaggtcgt 8461 ggacgtggag aaaagaggcc caggagtccc agtagtcagt catcatcatc cgggtctcca 8521 ccgcgcaggc cccctccagg tagaaggcca tttttccacc ctgtagggga agccgattat 8581 tttgaatacc accaagaagg tggcccagat ggtgagcctg acgtgccccc gggagcgata 8641 gagcagggcc ccgcagatga cccaggagaa ggcccaagca ctggaccccg gggtcagggt 8701 gatggaggca ggcgcaaaaa aggagggtgg tttggaaagc atcgtggtca aggaggttcc 8761 aacccgaaat ttgagaacat tgcagaaggt ttaagagctc tcctggctag gagtcacgta 8821 gaaaggacta ccgacgaagg aacttgggtc gccggtgtgt tcgtatatgg aggtagtaag 8881 acctcccttt acaacctaag gcgaggaact gcccttgcta ttccacaatg tcgtcttaca 8941 ccattgagtc gtctcccctt tggaatggcc cctggacccg gcccacaacc tggcccgcta 9001 agggagtcca ttgtctgtta tttcatggtc tttttacaaa ctcatatatt tgctgaggtt 9061 ttgaaggatg cgattaagga ccttgttatg acaaagcccg ctcctacctg caatatcagg 9121 gtgactgtgt gcagctttga cgatggagta gatttgcctc cctggtttcc acctatggtg 9181 gaaggggctg ccgcggaggg tgatgacgga gatgacggag atgaaggagg tgatggagat 9241 gagggtgagg aagggcagga gtgatgtaac ttgttaggag acgccctcaa tcgtattaaa 9301 agccgtgtat tcccccgcac taaagaataa atccccagta gacatcatgc gtgctgttgg 9361 tgtatttctg gccatctgtc ttgtcaccat tttcgtcctc ccaacatggg gcaattgccg 9421 gaacccttaa tataacttcg tataatgtat gctatacgaa gttattaggt ccctcgaaga 9481 ggttcactag cggatctcaa ttgggcatac ccatgttgtc acgtcactca gctccgcgct 9541 caacaccttc tcgcgttgga aaacattagc gacatttacc tggtgagcaa tcagacatgc 9601 gacggcttta gcctggcctc cttaaattca cctaagaatg ggagcaacca gcaggaaaag 9661 gacaagcagc gaaaattcac gcccccttgg gaggtggcgg catatgcaaa ggatagcact 9721 cccactctac tactgggtat catatgctga ctgtatatgc atgaggatag catatgctac 9781 ccggatacag attaggatag catatactac ccagatatag attaggatag catatgctac 9841 ccagatatag attaggatag cctatgctac ccagatataa attaggatag catatactac 9901 ccagatatag attaggatag catatgctac ccagatatag attaggatag cctatgctac 9961 ccagatatag attaggatag catatgctac ccagatatag attaggatag catatgctat 10021 ccagatattt gggtagtata tgctacccag atataaatta ggatagcata tactacccta 10081 atctctatta ggatagcata tgctacccgg atacagatta ggatagcata tactacccag 10141 atatagatta ggatagcata tgctacccag atatagatta ggatagccta tgctacccag 10201 atataaatta ggatagcata tactacccag atatagatta ggatagcata tgctacccag 10261 atatagatta ggatagccta tgctacccag atatagatta ggatagcata tgctatccag 10321 atatttgggt agtatatgct acccatggca acattagccc accgtgctct cagcgacctc 10381 gtgaatatga ggaccaacaa ccctgtgctt ggcgctcagg cgcaagtgtg tgtaatttgt 10441 cctccagatc gcagcaatcg cgcccctatc ttggcccgcc cacctactta tgcaggtatt 10501 ccccggggtg ccattagtgg ttttgtgggc aagtggtttg accgcagtgg ttagcggggt 10561 tacaatcagc caagttatta cacccttatt ttacagtcca aaaccgcagg gcggcgtgtg 10621 ggggctgacg cgtgccccca ctccacaatt tcaaaaaaaa gagtggccac ttgtctttgt 10681 ttatgggccc cattggcgtg gagccccgtt taattttcgg gggtgttaga gacaaccagt 10741 ggagtccgct gctgtcggcg tccactctct ttccccttgt tacaaataga gtgtaacaac 10801 atggttcacc tgtcttggtc cctgcctggg acacatctta ataaccccag tatcatattg 10861 cactaggatt atgtgttgcc catagccata aattcgtgtg agatggacat ccagtcttta 10921 cggcttgtcc ccaccccatg gatttctatt gttaaagata ttcagaatgt ttcattccta 10981 cactagtatt tattgcccaa ggggtttgtg agggttatat tggtgtcata gcacaatgcc 11041 accactgaac cccccgtcca aattttattc tgggggcgtc acctgaaacc ttgttttcga 11101 gcacctcaca tacaccttac tgttcacaac tcagcagtta ttctattagc taaacgaagg 11161 agaatgaaga agcaggcgaa gattcaggag agttcactgc ccgctccttg atcttcagcc 11221 actgcccttg tgactaaaat ggttcactac cctcgtggaa tcctgacccc atgtaaataa 11281 aaccgtgaca gctcatgggg tgggagatat cgctgttcct taggaccctt ttactaaccc 11341 taattcgata gcatatgctt cccgttgggt aacatatgct attgaattag ggttagtctg 11401 gatagtatat actactaccc gggaagcata tgctacccgt ttagggttaa caagggggcc 11461 ttataaacac tattgctaat gccctcttga gggtccgctt atcggtagct acacaggccc 11521 ctctgattga cgttggtgta gcctcccgta gtcttcctgg gcccctggga ggtacatgtc 11581 ccccagcatt ggtgtaagag cttcagccaa gagttacaca taaaggcaat gttgtgttgc 11641 agtccacaga ctgcaaagtc tgctccagga tgaaagccac tcagtgttgg caaatgtgca 11701 catccattta taaggatgtc aactacagtc agagaacccc tttgtgtttg gtcccccccc 11761 gtgtcacatg tggaacaggg cccagttggc aagttgtacc aaccaactga agggattaca 11821 tgcactgccc cgcgaagaag gggcagagat gtcgtagtca ggtttagttc gtccggggcg 11881 gggcatcgat cctctagagt cgacgctagc ggatccgacg ccgccatctc taggcccgcg 11941 ccggccccct cgcacagact tgtgggagaa gctcggctac tcccctgccc cggttaattt 12001 gcatataata tttcctagta actatagagg cttaatgtgc gataaaagac agataatctg 12061 ttctttttaa tactagctac attttacatg ataggcttgg atttctataa gagatacaaa 12121 tactaaatta ttattttaaa aaacagcaca aaaggaaact caccctaact gtaaagtaat 12181 tgtgtgtttt gagactataa atatcccttg gagaaaagcc ttgtttgggc cgactccagt 12241 ggtaatctac ttcaagagag tagattacca ctggagtctt ttttggaatt cctgcagccc 12301 gggggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 12361 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 12421 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 12481 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 12541 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 12601 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 12661 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 12721 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 12781 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 12841 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 12901 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 12961 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 13021 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 13081 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 13141 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 13201 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 13261 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 13321 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 13381 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 13441 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 13501 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 13561 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 13621 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 13681 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 13741 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 13801 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 13861 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 13921 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 13981 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 14041 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 14101 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 14161 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 14221 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 14281 ccgaaaagtg ccacctgg //