LOCUS       Exported                7376 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBabepuro-myc-ER
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7376)
  AUTHORS   Ricci MS, Jin Z, Dews M, Yu D, Thomas-Tikhonenko A, Dicker DT, 
            El-Deiry WS
  TITLE     Direct repression of FLIP expression by c-myc is a major determinant
            of TRAIL sensitivity.
  JOURNAL   Mol Cell Biol. 2004 Oct . 24(19):8541-55.
  PUBMED    15367674
REFERENCE   2  (bases 1 to 7376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7376
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             942..1358
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1288..1310
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1326..1342
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1372..2688
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     primer_bind     complement(3704..3724)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3709..4038
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3889..4024
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3951..3970
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4048..4647
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4048..4067)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4544..4564
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4870..5339
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5469..5486)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5640..6228)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5720..5739)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6388..7248)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7011..7030
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7249..7353)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
      901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
      961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatccac catgcccctc
     1381 aacgttagct tcaccaacag gaactatgac ctcgactacg actcggtgca gccgtatttc
     1441 tactgcgacg aggaggagaa cttctaccag cagcagcagc agagcgagct gcagcccccg
     1501 gcgcccagcg aggatatctg gaagaaattc gagctgctgc ccaccccgcc cctgtcccct
     1561 agccgccgct ccgggctctg ctcgccctcc tacgttgcgg tcacaccctt ctcccttcgg
     1621 ggagacaacg acggcggtgg cgggagcttc tccacggccg accagctgga gatggtgacc
     1681 gagctgctgg gaggagacat ggtgaaccag agtttcatct gcgacccgga cgacgagacc
     1741 ttcatcaaaa acatcatcat ccaggactgt atgtggagcg gcttctcggc cgccgccaag
     1801 ctcgtctcag agaagctggc ctcctaccag gctgcgcgca aagacagcgg cagcccgaac
     1861 cccgcccgcg gccacagcgt ctgctccacc tccagcttgt acctgcagga tctgagcgcc
     1921 gccgcctcag agtgcatcga cccctcggtg gtcttcccct accctctcaa cgacagcagc
     1981 tcgcccaagt cctgcgcctc gcaagactcc agcgccttct ctccgtcctc ggattctctg
     2041 ctctcctcga cggagtcctc cccgcagggc agccccgagc ccctggtgct ccatgaggag
     2101 acaccgccca ccaccagcag cgactctgag gaggaacaag aagatgagga agaaatcgat
     2161 gttgtttctg tggaaaagag gcaggctcct ggcaaaaggt cagagtctgg atcaccttct
     2221 gctggaggcc acagcaaacc tcctcacagc ccactggtcc tcaagaggtg ccacgtctcc
     2281 acacatcagc acaactacgc agcgcctccc tccactcgga aggactatcc tgctgccaag
     2341 agggtcaagt tggacagtgt cagagtcctg agacagatca gcaacaaccg aaaatgcacc
     2401 agccccaggt cctcggacac cgaggagaat gtcaagaggc gaacacacaa cgtcttggag
     2461 cgccagagga ggaacgagct aaaacggagc ttttttgccc tgcgtgacca gatcccggag
     2521 ttggaaaaca atgaaaaggc ccccaaggta gttatcctta aaaaagccac agcatacatc
     2581 ctgtccgtcc aagcagagga gcaaaagctc atttctgaag aggacttgtt gcggaaacga
     2641 cgagaacagt tgaaacacaa acttgaacag ctacggaact cttgtgcgaa ttccgatcca
     2701 cgaaatgaaa tgggtgcttc aggagacatg agggctgcca acctttggcc aagccctctt
     2761 gtgattaagc acactaagaa gaatagccct gccttgtcct tgacagctga ccagatggtc
     2821 agtgccttgt tggatgctga accgcccatg atctattctg aatatgatcc ttctagaccc
     2881 ttcagtgaag cctcaatgat gggcttattg accaacctag cagataggga gctggttcat
     2941 atgatcaact gggcaaagag agtgccaggc tttggggact tgaatctcca tgatcaggtc
     3001 caccttctcg agtgtgcctg gctggagatt ctgatgattg gtctcgtctg gcgctccatg
     3061 gaacacccgg ggaagctcct gtttgctcct aacttgctcc tggacaggaa tcaaggtaaa
     3121 tgtgtggaag gcatggtgga gatctttgac atgttgcttg ctacgtcaag tcggttccgc
     3181 atgatgaacc tgcagggtga agagtttgtg tgcctcaaat ccatcatttt gcttaattcc
     3241 ggagtgtaca cgtttctgtc cagcaccttg aagtctctgg aagagaagga ccacatccac
     3301 cgtgtcctgg acaagatcac agacactttg atccacctga tggccaaagc tggcctgact
     3361 ctgcagcagc agcatcgccg cctagctcag ctccttctca ttctttccca tatccggcat
     3421 atgagtaaca aacgcatgga gcatctctac aacatgaaat gcaagaacgt ggtacccctc
     3481 tatgacctgc tcctggagat gttggatgcc caccgccttc atgccccagc cagtcgcatg
     3541 ggagtgcccc cagaggagcc cagccagacc cagctggcca ccaccagctc cacttcagca
     3601 cattccttac aaacctacta catacccccg gaagcagagg gcttccccaa cacgatctga
     3661 gagctcctac tagtggatct cccatcgatg gggtcgaccc tgtggaatgt gtgtcagtta
     3721 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     3781 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3841 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     3901 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     3961 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4021 ggcctaggct tttgcaaaaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc
     4081 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     4141 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     4201 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     4261 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     4321 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     4381 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     4441 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     4501 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     4561 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     4621 tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa
     4681 aggagcgcac gaccccatgc atcgcgataa aataaaagat tttatttagt ctccagaaaa
     4741 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
     4801 gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag
     4861 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     4921 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
     4981 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc
     5041 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
     5101 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
     5161 gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag
     5221 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
     5281 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac
     5341 atgcagcatg tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca
     5401 tagttgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     5461 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5521 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5581 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5641 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5701 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5761 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5821 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5881 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5941 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6001 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6061 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6121 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6181 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6241 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6301 atgagattat caaaaaggat cttcacctag atccttttgc ggccgcaaat caatctaaag
     6361 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     6421 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     6481 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     6541 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     6601 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     6661 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     6721 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     6781 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     6841 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     6901 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     6961 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     7021 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     7081 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     7141 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     7201 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     7261 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     7321 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//
